Separation of xanthone and vitamin E from Calophyllum inophyllum leaf


  • Hakun Wirawasista Aparamarta Institut Teknologi Sepuluh Nopember (ITS)
  • Safrina Hapsari Institut Teknologi Sepuluh Nopember (ITS)
  • Reinaldi Ismawan Institut Teknologi Sepuluh Nopember (ITS)
  • Violita Anggraeni Institut Teknologi Sepuluh Nopember (ITS)
  • Arief Widjaja Institut Teknologi Sepuluh Nopember (ITS)
  • Tri Widjaja Institut Teknologi Sepuluh Nopember (ITS)
  • Yi-Hsu Ju National Taiwan University of Science and Technology
  • Setiyo Gunawan Institut Teknologi Sepuluh Nopember (ITS)



C. inophyllum leaf, polarity, recovery, soxhlet solvent extraction, vitamin E


Calophyllum inophyllum (nyamplung) has many advantages start from stems, leaves, roots, to seeds. Indonesian society generally only know that seeds can produce biodiesel. Nyamplung leaves have bioactive compounds, such as xanthone that can be used for medical purposed. Recently, quantification of xanthone and vitamin E in the nyamplung leaf still doesn’t exist. This research aims to isolate and quantify the content of xanthone and vitamin E from the crude nyamplung leaf. The separation is done through liquid-liquid extraction with methanol and petroleum ether (PE) to obtain two layers namely non-polar fraction (PE fraction) and polar fraction (methanol fraction). The mass ratio of solvent mixture-to-crude extract (10,30, 50 and 70) and the mass ratio of PE-to-methanol (1 and 3) were applied and analyzed by TLC, GC-MS, and UV-Vis. Xanthones (0.150 equilibrium constant, 15.16% purity and 94.43% recovery) were obtained in the methanol fraction based on the mass ratio of solvent mixture-to-crude extract of 70 and mass ratio of PE-to-methanol at 1.From GC-MS, Xanthones were detected in the methanol fraction with quality of 94% and Vitamin E was detected in the petroleum ether fraction with quality of 99%.

Author Biographies

Hakun Wirawasista Aparamarta, Institut Teknologi Sepuluh Nopember (ITS)

Department of Chemical Engineering, Faculty of Industrial Engineering

Safrina Hapsari, Institut Teknologi Sepuluh Nopember (ITS)

Department of Chemical Engineering, Faculty of Industrial Engineering

Reinaldi Ismawan, Institut Teknologi Sepuluh Nopember (ITS)

Department of Chemical Engineering, Faculty of Industrial Engineering

Violita Anggraeni, Institut Teknologi Sepuluh Nopember (ITS)

Department of Chemical Engineering, Faculty of Industrial Engineering

Arief Widjaja, Institut Teknologi Sepuluh Nopember (ITS)

Department of Chemical Engineering, Faculty of Industrial Engineering

Tri Widjaja, Institut Teknologi Sepuluh Nopember (ITS)

Department of Chemical Engineering, Faculty of Industrial Engineering

Yi-Hsu Ju, National Taiwan University of Science and Technology

Department of Chemical Engineering 

Setiyo Gunawan, Institut Teknologi Sepuluh Nopember (ITS)

Department of Chemical Engineering, Faculty of Industrial Engineering


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