Bacillus amyloliquefaciens Activates the Basal Defense of Chinese cabbage Against Alternaria brassicicola
DOI:
https://doi.org/10.11113/mjfas.v21n6.4454Keywords:
Biocontrol mechanism, black leaf spot disease, callose deposition, swollen conidiaAbstract
Alternaria brassicicola is a necrotrophic plant pathogen that causes substantial damage to a broad range of host plants, especially Brassica species. Various strategies were used to control this disease, including applying antagonistic microorganisms. In this study, we evaluate two strains of Bacillus amyloliquefaciens (PMB04 and PMB05) as biological agents to control black leaf spot disease caused by A. brassicicola on Chinese cabbage (Brassica rapa var. chinensis). We found these two strains inhibited the mycelial growth and spore germination of A. brassicicola, and suppressed the disease severity on Chinese cabbage. However, PMB04 showed a better in vitro inhibitory effect than PMB05, but PMB05 has a better biocontrol efficacy than PMB04. To gain more insight into the converse between these two strains' inhibitory effect and biocontrol efficacy, the plant immune response was intensified with the B. amyloliquefaciens bacterial cell and cultural filtrate. The results exhibited that both forms of PMB05 significantly intensify the plant immunity in leaf tissue upon the inoculation of A. brassisicola spores rather than those of PMB04. This suggests that B. amyloliquefaciens strain PMB04 and PMB05 have different biocontrol mechanisms.
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Copyright (c) 2025 Gabryna Auliya Nugroho, Yi-Hsien Lin, Tzou-Chi Huang, Yulia Nuraini, Li'aini Arrohmatus Syafaqoh
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