Biodegradation of crude oil by Ralstonia pickettii under high salinity medium


  • Adi Setyo Purnomo Institut Teknologi Sepuluh Nopember
  • Hamdan Dwi Rizqi Institut Ilmu Kesehatan Bhakti Wiyata Kediri
  • Lia Harmelia Institut Teknologi Sepuluh Nopember
  • Silmi Diah Anggraeni Institut Teknologi Sepuluh Nopember
  • Ranny Etna Melati Institut Teknologi Sepuluh Nopember
  • Zulfah Hernida Damayanti Institut Teknologi Sepuluh Nopember
  • Ova Mar’atus Shafwah Institut Teknologi Sepuluh Nopember
  • Fransisca Cahyaning Kusuma Institut Teknologi Sepuluh Nopember



Biodegradation, Ralstonia pickettii, Crude oil, High Salinity Medium.


Bacterium Ralstonia pickettii has ability to survive and thrive in low nutrient condition as well as a capability to remediate some pollutants and using them as carbon and energy source. In this study, the ability of R. pickettii on biodegradation of crude oil under high salinity medium was investigated. R. pickettii was pre-incubated in nutrient broth (NB) medium and then, washed and transferred to artificial seawater medium. Crude oil was added to each culture and incubated for 7 and 14 days. The biodegradation of crude oil was analysed using Gas chromatography mass spectrometry (GC-MS). The result showed that R. pickettii had successfully degraded the crude oil in the high salinity artificial seawater. The incubation on 7 and 14 days did not show a significant effect on the number of the degraded compounds. The optimum recovery percent was obtained from the derivation of 2,6,10,14-tetramethyl hexadecane with the recovery percentage of 12.7% and 16.0% for 7 and 14 days respectively. This study indicates that R. picketti can be potentially used for bioremediation of crude oil under high salinity environments.

Author Biographies

Adi Setyo Purnomo, Institut Teknologi Sepuluh Nopember

Department of Chemistry

Hamdan Dwi Rizqi, Institut Ilmu Kesehatan Bhakti Wiyata Kediri

Faculty of Science Technology and Analysis

Lia Harmelia, Institut Teknologi Sepuluh Nopember

Silmi Diah Anggraeni, Institut Teknologi Sepuluh Nopember

Ranny Etna Melati, Institut Teknologi Sepuluh Nopember

Department of Chemistry

Zulfah Hernida Damayanti, Institut Teknologi Sepuluh Nopember

Department of Chemistry

Ova Mar’atus Shafwah, Institut Teknologi Sepuluh Nopember

Department of Chemistry


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