Antioxidant and antibacterial activities of Ischaemum indicum leaves extracted using different solvents


  • Fithrotul Auwaliyah Institut Teknologi Sepuluh Nopember (ITS)
  • Hamdan Dwi Rizqi Institut Teknologi Sepuluh Nopember (ITS)
  • Adi Setyo Purnomo Institut Teknologi Sepuluh Nopember (ITS)
  • Sri Fatmawati Institut Teknologi Sepuluh Nopember (ITS)



Ischaemum indicum, ABTS, DPPH, Antioxidant, Antibacterial.


The aim of this study was to evaluate antioxidant and antibacterial activities of Ischaemum indicum leaves extracts based on different polarity solvents: water, methanol, ethyl acetate, dichloromethane, and n-hexane. The antioxidant activity was evaluated using ABTS and DPPH methods, while the antibacterial activity was determined by broth dilution method against Pseudomonas aeruginosa and Bacillus subtilis. The results showed that water and methanol extracts of I. indicum gave the highest antioxidant activity in ABTS assay with an IC50 73.80 and 46.32 µg/mL, respectively. On the other hand, The IC50 on DPPH assay for  water extracts was 117.51 µg/mL, this value was higher than that of methanol extract with no inhibition. Methanol and water extracts showed the antibacterial activity against B. subtilis with inhibition of 48.62 and 45.11%, whereas inhibition of P. aeruginosa were 31.95 and 30.31%, respectively. This study denoted that I. indicum is a new potential candidate as antioxidant source.

Author Biographies

Fithrotul Auwaliyah, Institut Teknologi Sepuluh Nopember (ITS)

Department of Chemistry, Faculty of Natural Sciences, Institut Teknologi Sepuluh Nopember (ITS).


Hamdan Dwi Rizqi, Institut Teknologi Sepuluh Nopember (ITS)

Department of Chemistry, Faculty of Natural Sciences, Institut Teknologi Sepuluh Nopember (ITS).

Adi Setyo Purnomo, Institut Teknologi Sepuluh Nopember (ITS)

Department of Chemistry, Faculty of Natural Sciences, Institut Teknologi Sepuluh Nopember (ITS).

Sri Fatmawati, Institut Teknologi Sepuluh Nopember (ITS)

Department of Chemistry, Faculty of Natural Sciences, Institut Teknologi Sepuluh Nopember (ITS).


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