Antioxidant and antibacterial activities of Ischaemum indicum leaves extracted using different solvents

Fithrotul Auwaliyah, Hamdan Dwi Rizqi, Adi Setyo Purnomo, Sri Fatmawati

Abstract


The aim of this study was to evaluate antioxidant and antibacterial activities of Ischaemum indicum leaves extracts based on different polarity solvents: water, methanol, ethyl acetate, dichloromethane, and n-hexane. The antioxidant activity was evaluated using ABTS and DPPH methods, while the antibacterial activity was determined by broth dilution method against Pseudomonas aeruginosa and Bacillus subtilis. The results showed that water and methanol extracts of I. indicum gave the highest antioxidant activity in ABTS assay with an IC50 73.80 and 46.32 µg/mL, respectively. On the other hand, The IC50 on DPPH assay for  water extracts was 117.51 µg/mL, this value was higher than that of methanol extract with no inhibition. Methanol and water extracts showed the antibacterial activity against B. subtilis with inhibition of 48.62 and 45.11%, whereas inhibition of P. aeruginosa were 31.95 and 30.31%, respectively. This study denoted that I. indicum is a new potential candidate as antioxidant source.


Keywords


Ischaemum indicum, ABTS, DPPH, Antioxidant, Antibacterial.

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Ajaib, M., Khalid, M. K., Shahnaz, P., Shazia, S. Antimicrobial and antioxidant activities of echinochola colona (Linn.) link and sporobolus coroamdelianus (Retz) Kunth. Journal of the Chemical Society of Pakistan., vol. 35, no. 3, pp. 961-966, 2013.

Akuodor, G. C., Essien, A. D., Ibrahim, J. A., Bassey, A., Akpan, J. L., Ikoro, N. C., Onyewenjo, S. C. Phytochemical and antimicrobial properties of the methanolic extract of Bombax buanopozense leaf and root. Asian Journal of Medical Sciences, vol. 2, pp. 190-194, 2011.

Aqil, F. I., Ahmed, I., Mehmood, Z. Antioxidant and free radical scavenging properties of twelve traditionally used Indian medical plants. Turkish Journal of Biology, vol. 30, pp. 177-183, 2006.

Arias, M. E., Gomez, J. D., Cudmani, N. M., Vattuone, M. A., Isla, M. I. Antibacterial activity of ethanolic and aqueous extracts of Acacia aroma Gill Ex Hook et Arn. Life Sciences, vol. 75, pp. 191-202, 2004.

Awah, F. M., Uzoegwu, P. N., Oyugi, J. O., Rutherford, J., Ifeonu, P., Yao, X., Fowke, K. R., Eze, M. O. Free radical scavenging activity and immunomodulatory effect of Stachytarpheta angustifolia leaf exract. Food Chemistry, vol. 119, pp. 1409-1416, 2010.

Bagchi, K., Puri, S. Free radicals and antioxidants in health and diseases. Eastern Mediterranean Health Journal, vol. 4, pp. 350-360, 1998.

Beckman, K. B., Ames, B. N. The free radical theory of aging matures. Physiological Reviews, vol. 78, pp. 547-581, 1998.

Chon, S. U., Heo, B. G., Park, Y. S., Kim, D. K., Gorinstein, S. Total phenolic level, antioxidant activities and cytotoxicity of young sprouts of some traditional Korean salad plants, Plant Foods for Human Nutrition, vol. 62, pp. 167-179, 2009.

Cujic, N., Savikin, K., Jankovic, T., Pljevljakusic, D., Zdunic, G., Ibric, S. Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique. Food Chemistry, vol. 194, pp. 135–142, 2016.

Diastuti, H., Syah, Y. M., Juliawaty, L. D., Singgih, M. Antibacterial Curcuma xanthorrhiza extract and fraction. Journal of Mathematical and Fundamental Sciences, vol. 46, no. 3, pp 224, 2014.

Emam, S. A., Asilii, J., Mohagheghi, Z., Hassanzadeh, M. K. Antioxidant activity of leaves and fruits of Iranian conifers. RSC Advances, vol. 4, no. 3, pp. 313-319, 2007.

Fankam, A. G., Kuiate, J. R., Kuete, V. Antibacterial activities of Belschmiedia obscura and six other Cameroonian medical plants against multi-drug resistant Gram-negative phenotypes. BMC Complementary and Alternative Medicine, vol. 14, pp. 241, 2014.

Fauzia, D. N., Fatmawati, S., Ersam, T. New prenylated stilbenes and antioxidant activities of Cajanus cajan (L.) Millsp. (Pigeon pea). Indonesian Journal of Chemistry, vol. 16, no.2, pp. 151-155. 2016.

Fitriana, W. D., Ersam, T., Shimizu, K., Fatmawati, S. Antioxidant activities of moringa oliefera extracts. Indonesian Journal of Chemistry, vol. 16, no.3, pp. 297-301. 2016.

Hark, L., Deen, D. Nutrion for Life. London: Dorling Kindersley Publishers, pp. 213, 2006.

Hassan, A., Rahman, S., Deeba, F., Mahmud, S. Antimicrobial activity of some plant extracts having hepatoprotective effects. Journal of Medicinal Plants Research, vol. 3, no. 1, pp. 20-23, 2009.

Hidayati, M. D., Ersam, T., Shimizu, K.., Fatmawati, S. Antioxidant activity of Syzygium polynthum extracts. Indonesian Journal of Chemistry, vol. 17, no.1, pp. 49-53. 2017.

Ivanova, E., Atanasova, N., Kungulovski, D. Antimicrobial activities of laboratory produced essential oil solutions against five selecte fungal strains. Journal of Nature and Science, vol. 124, pp 171, 2013.

Jiang, L. Comparison of disk diffusion, agar dilution, and broth microdilution for antimicrobial susceptibility testing of five chitosans. Thesis, Fujian Agricultural and forestry University, China, 2011.

Kadiman, K. Buku Putih Penelitian, Pengembangan dan Penerapan Iptek Bidang Kesehatan dan Obat. Kementrian Negara Riset dan Teknologi Republik Indonesia, Jakarta, 2006.

Kellogg., Elizabeth, A. The Families and Genera of Vascular Palnt, Volume XIII : Flowering Plans Monocots Poaceae. Switzerland: Springer International Publishing, vol. 87-90, 2015.

Khan, U. A., Rahman, H., Niaz, Z., Qasim, M., Khan, J., Tayyaba. Antibacterial activity of some medical plants against selected human pathogenic bacteria. European Journal of Microbiology and Immunology, vol. 3, pp. 272, 2013.

Kumar, R. S., Rajkapoor, B., Perumal, P. Antioxidant activities of Indigofera cassioides Rottl. Ex. DC. using various in vitro assay models. Asian Pacific Journal of Tropical Biomedicine, pp. 256-261, 2012.

Li, H. B., Cheng, K., Wong, C., Fan, K., Chen, F., Jiang, Y. Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae. Food Chemistry, vol. 102, pp. 771-776, 2007.

Luis, K. M. Isolation and partial characterization of the antibacterial compounents of Cogon (Imperata cylindrical) Roots. Thesis. Mapua Institute of Technology, pp. 19-27, 2015.

Nasir, A., Ali, S. I. Flora of Pakistan. Poaceae, vol. 143, no. 2, pp. 142-196, 1982.

Nguyen, V. T., Bowyer, M. C., Vuong, Q. V., Altena, L. A. V., Scarlet, J. C. H. Phytochemicals and antioxidant capacity of Xao tam phan (Paramignya trimera) rot as affected by various solvents and extractions methods. Industrial Crops and Products, vol. 67, pp. 192–200, 2015.

Nithya, P., Madhavi, C. Antioxidant activity of 3-arylidene-4-piperidones in the 1,1-diphenyl-2-picrylhydrazyl scavenging assay. Journal of Taibah University for Science, vol. 11, pp. 40-45, 2017.

Nostro, A., Germano, M. P., D’Angelo, V., Marino, A., Cannatelli, M. A. N., Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Letters in Applied Microbiology, vol. 30, pp. 379-384, 2000.

Pyne, S.G., Liawruangrath, S., Garson, M., Khamsan, S., “Antimalarial, Anticancer, Antimicrobial activities and chemical constituents of essential oil from the aerial parts of Cyperus kyllingia Endl,” M Garson. Rec. Natural Prod, vol. 5, no. 4, pp. 324-327, 2011.

Pyrzynska, K., Pekal, A. Aplication of free radical diphenylpicrylhydrazyl (DPPH) to estimate the antioxidant capacity of food samples. Anal Methods, vol. 5, pp. 4288-95, 2013.

Prior, R. L., Wu, X., Schaich, K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietery supplements. Journal of Agricultural and Food Chemistry. vol. 53, no. 10, pp. 4290-4302, 2005.

Raj, S., Berthomier, M., Babu, M. A., Karthlkeyan, S., Sivakumar, A., Gothandam, K. M. Antioxidant, antibacerial and anti-proliferative activity and phytochemical analysis of selected medical plants from Dasapushpam of Kerala. International Journal of Pharmaceutical Sciences Review and Research, vol. 23, no. 1, pp. 172-179, 2013.

Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, vol. 26, no. 9-10, pp. 1231–1237, 1999.

Shalaby, E. A., Shanab, S. M. M.Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis. Indian Journal of Geo-Marine Sciences, Vol. 42, no. 25. pp 556-564, 2012.

Sultana, B., Anwar, F., Przybylski, R. Antioxidant activity of phenolics components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam. Trees. Food Chemistry, vol. 104, no. 3, pp. 1106-1114, 2007.

Zhou, H. C., Lin, Y. M., Wei, S. D., Tam, N. F. Y. Structural diversity and antioxidant activity of condensed tannins fractionated from mangosteen pericarp. Food Chemistry, vol. 129, no. 4, pp. 1710–1720, 2011.

Zurowska, D. M., Wenta, W. A comparison of ABTS and DPPH methods for assessing the total antioxidant capacity of human milk. Journal Food Sciences and Nutrition Acta Scientiarum Polonorum, vol. 11, no. 1, pp. 83-89, 2012.




DOI: https://doi.org/10.11113/mjfas.v15n3.1037

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