LC-TOF-MS/MS-Based Phytochemical Profiling and Antibacterial Activity of Nephelium lappaceum Leaf Extracts
DOI:
https://doi.org/10.11113/mjfas.v22n5.5530Abstract
Medicinal plants are a valuable source of pharmaceuticals, as their phytochemical constituents exhibit diverse biological activities with fewer side effects and lower possibility of resistance development compared to synthetic drugs. This study investigated the antibacterial properties of methanolic and chloroform-methanol-water (CMA) extracts from the leaves of Nephelium lappaceum L. from three cultivars - R191, AG28, and GB44 against Staphylococcus aureus and Escherichia coli, using agar well diffusion method. The methanolic extracts exhibited significantly higher antibacterial activity compared to CMA extracts. To tentatively identify metabolites potentially associated with the observed antibacterial activity, the methanolic extracts were analysed using LC-TOF-MS/MS techniques. The Liquid Chromatography-Time-of-Flight Mass Spectrometry/Mass Spectrometry (LC-TOF-MS/MS) analysis tentatively identified three, ten, and nine compounds in R191, AG28, and GB44 respectively, which were subsequently categorized as flavonoids, tryptophan alkaloids, and triterpenoids. Two compounds, Procyanidin B2, and Quercetin were detected in all extracts. These findings highlight the potential of N. lappaceum leaf extracts as a source of natural antibacterial agents and warrant further in vivo studies for the development of novel antibacterial drugs.
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