Optimal concentration of mangrove (Rhizophora mucronata) leaf and propagule based natural dye

Authors

  • Delianis Pringgenies Diponegoro University
  • Ervia Yudiati Diponegoro University
  • Ria Azizah Tri Nuraini Diponegoro University
  • Endang Sri Susilo Diponegoro University
  • Edia Rahayuningsih Gajah Mada University

DOI:

https://doi.org/10.11113/mjfas.v14n1-2.1011

Keywords:

Column chromatography, dye concentration, leaves, Rhizophora mucronata

Abstract

This research aims to discover total extract level from leaf and propagule of mangrove Rhizophora mucronata, Baume relationship and extract level from leaf and propagule of mangrove, the result of color extract for fabric cotton, phytochemical screening of active compounds contained in mangroves and dye application by fractionation method using column chromatography. The methods employed in this study by means of Baume scale, gravimetric analysis of extract contents, phytochemical screening, and column gravity chromatography separated color of the pigment. The results showed that the higher the level of extract, the greater the mass of the species and the higher the level of extract, the color will be darker. During the soxhlet extraction process, water contents of the material were at 60% for fresh leaves, 18% for dehydrated leaves, 16% for mangrove fruit propagule, and 14% for mangrove branch propagule. Average dye contents were measured at 5% for fresh leaves, 22% for dehydrated leaves, 17% for mangrove fruit propagule, and 21% for mangrove branch propagule. The colors resulted from treatment of the samples using limestone, alum and lotus solutions from each mangrove leaf and propagule sample were different from white to dark brown. The optimal concentration of dye found in this study were 0.0071 g/mL for fresh leaves, 0.0080 g/mL for dehydrated leaves, 0.0255 g/mL for fruit propagule, and 0.0275 g/mL for branch propagule. Phytochemical test results also show that the pigment associated to the blackish-green hue was tannin, and that which was associated with red was flavonoid and quinone. The result of column chromatography showed that on leaf waste and skin is found in blackish green color and on the skin can be found in red.

References

Agustina, T. E., Nurisman, E., Prasetyowati, Haryani, N., Cundari, L., Novisa, A., Khristina, O. 2011. Pengolahan air limbah pewarna sintetis dengan menggunakan reagen fenton. In: Prosiding Seminar Nasional AVoER ke-3, 26-27 October. Palembang, 260-266.

Bogoriani, N. W. 2010. Ekstraksi zat warna alami campuran biji pinang, daun sirih, gambir dan pengaruh penambahan KMnO4 terhadap pewarna kayu jenis Albasi. Jurnal Kimia. 4(2). Juli. P. 125-134.

Harbone, J. B. 1987. Metode Fitokimia. Penuntun Cara Modern Menganalisa Tumbuhan. Terbitan Kedua. ITB, Bandung, 6-15.

Harborne, J. B. 1984. Phytochemical Methods: A guide to modern technique of plant analysis. (2nd ed.). Chapman and Hall, London. 19, 37–168.

Indrianingsih, A. W., Darsih, C. 2013. Natural dyes from plants extract and its applications in Indonesian textile small medium scale enterprise. Technical Implementation Unit for Chemical Engineering Processes, 11(1), 16-22.

Neliyanti, Idiawati, N. 2014. Ekstraksi dan uji stabilitas zat warna alami dari buah lakum (Cayratia trifolia (L.) Domin). Jurnal Kimia Khatulistiwa, 3(2), 30-37.

Pringgenies. D., Supriyantini, E., Azizah, R., Hartati, R., Irwani, Radjasa, O. K. 2013. Application of mangrove as natural dye for batik diservication at Gemawang Village, Semarang Regency [Conference Program and Abstracts]. In: 2nd Natural Pigments Conference for South-East Asia (NP-SEA). 12-13 July. Universitas Ma Chung, Malang, pp.68.

Pringgenies. D, Pratiwi, A. H. D., Yudiati, E., Azizah, R., Susilo, E. S. 2017. Biopigment tracing of mangrove Rhizophora mucronata leaf and bark waste and its application for batik dyeing by multiple fixations. In: Proceeding of The 7th Annual Basic Science International Conference. 7-8 March. Malang, Indonesia, 180-111. Retrieved from: http://basic.ub.ac.id/files/proceeding/PROCEEDINGS-BASIC-2017-VOL1.pdf

Yuniwati M., A. W. Kusuma, F. Yunanto. 2012. Optimasi kondisi proses ekstraksi zat pewarna dalam daun suji dengan pelarut etanol. Prosiding Seminar Nasional Aplikasi Sains & Teknologi (SNAST) Periode III. 3 November. Yogyakarta, Indonesia. A.258 –263.

Yusuf. M, F. Mohammad., Shabbir. 2017. Eco-friendly and effective dyeing of wool with anthraquinone colorants extracted from Rubia cordifolia roots: Optimization, colorimetric and fastness assay. Journal of King-Saud University - Science. 29, 137-144.

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Published

30-04-2018