The anthocyanin pigment extract from red rose as antibacterial agent


  • Elfi Anis Saati Muhammadiyah University of Malang
  • Annisa Dyah Pusparini Muhammadiyah University of Malang
  • Mochammad Wachid Muhammadiyah University of Malang
  • Sri Winarsih Muhammadiyah University of Malang



Anthocyanin pigment, antibacterial, extract, red rose


Red rose consisting of anthocyanin pigment has been used as an antibacterial agent. However, there is no study on the anthocyanin pigment extract from red rose as the antibacterial agent. The effectiveness of the antibacterial agent can be affected by the solvent extraction and the flower shelf life. Here, we report the effects of solvent extraction and red rose (Rosa sp.) shelf life on the antibacterial activity. Red rose concentrated extraction and randomized complete block design factorial was carefully used with factors of long display and solvent extraction. The extraction solvent (P) comprised of water, ethanol, and mixture of water-ethanol (1 : 1), while the red rose shelf life (M) consisted of 0, 2, 4 and 6 days. Moreover, pH, antioxidant activity and minimum bactericidal concentration (MBC) with four variations of concentrated concentration (100%, 50%, 25% and 12.5%) on Escherichia coli, Salmonella thypi, and Pseudomonas sp. were analyzed. After two days of shelf life using water as the solvent for extraction, the antioxidant activity achieved 79% at pH of 2.5. It was also demonstrated that it was able to kill all the investigated bacterias, which were Escherichia coli, Salmonella thypi, and Pseudomonas sp. with concentrated concentrations of 100%, 50% and 25%. When the concentrated concentration was 12.5%, the MBC value was constantly found to be 1.39 × 108 cfu/g for Escherichia coli and 9.53 × 107 cfu/g for Salmonella thypi.


Blando, F., Calabriso, N., Berland, H., Maiorano, G., Gerardi, C., Carluccio, M. A., and Andersen, Ø. M. 2018. Radical scavenging and anti-inflammatory activities of representative anthocyanin groupings from pigment-rich fruits and vegetables. Int. J. Mol. Sci. 19(1), 169.

Böhm, R. 2009. Antimicrobial of Thai traditional medicinal plants extract incorporated alginate-tapioca starch based edible films against food related bacterial including foodborne pathogens. PhD Dissertation. Faculty of Agricultural Sciences, University of Hohenheim, Pattani.

Baydar, G. N., Özkan G., and Sağdiç O., 2004. Total phenolic contents and antibacterial activities of grape (Vitis vinifera L.) extracts. Food Control. 15(5), 335–339.

Handarini, 2014. Rosella (Hibiscus sabdariffa Linn.) extract potention as natural preservatives in fresh chicken as substitute for formalin. Jurnal Agroknow. 2(1), 1–70.

Ningtyas, R. 2010. Antioxidant and antibacterial of Kecombrang leaf extract (Etlingera elatoir (Jack) R.M. Smith) as natural preservative against Escherichia coli and Staphylococcus aureus. Master’s thesis. Faculty of Science and Technology, State Islamic University (UIN) Syarif Hidayatullah, Jakarta, Indonesia.

Özkan G., Sağdiç O., Baydar N. G., Baydar, H., 2004. Antioxidant and antibacterial activities of Rosa damascene flower extracts. Food Sci. Technol. Int. 10(4) 277–281.

Raghvendra, Sharma, V., Shakya, A., Hedaytullah, M. D., Arya, G. S., Mishra, A., Gupta, A. D., Pachpute, A.P., Patel, D. 2011. Chemical and potential aspects of anthocyanins – A water-soluble vacuolar flavonoid pigments: A review. Int. J. Pharm. Sci. Rev. Res. 6(1), 28–33.

Samber, L. N., Semangun, H., and Prasetyo, B. 2013. Anthocyanin characteristic as natural coloring. National seminar of 10th Biology Education FKIP UNS 2013. 6 July. University of Sebelas Maret, Surakarta, Indonesia, 1-4.

Saati, E. A. 2016. Antioxidant power of rose anthocyanin pigment. ARPN J. Eng. Appl. Sci. 11(17), 10201–10204.

Saati, E. A. 2016. Identification of glycone types in the crown flower of Batu local roses using LC-MS analysis. ARPN J. Eng. Appl. Sci. 11(21), 12729–12734.

Saati, E. A., Aisyah, R., Wachid, M., and Winarsih, S. 2014. Non-alcoholic extraction of rose pigment as halal and safe natural colorant and bioactive compound. JISTECS. 2(2), 65–70.

Warziki, N. R. 2015. The effect of tamarind (Tamarindus indica L.) extract concentration and submersion time to bandeng (Chanos chanos F.) fillet quality. Master’s thesis. Faculty of Agriculture and Animal Husbandry, University of Muhammadiyah Malang, Indonesia.