Antioxidant and antibacterial activities of Ananas comosus peel extracts
DOI:
https://doi.org/10.11113/mjfas.v14n2.928Keywords:
Ananas comosus peel, Antioxidant, Antibacterial, ABTS, DPPHAbstract
Antioxidant and antibacterial activities of Ananas comosus peel extracts (n-hexane, dichloromethane, ethyl acetate, methanol and water) had been evaluated. The antioxidant activity had been evaluated by using DPPH (2,2-diphenyl-2-picrylhydrazyl) and ABTS (2,2’-azinobis-(3-ethylbenzothiazole-6-sulfonic acid) methods, while antibacterial activity has been evaluated by using broth dilution method. The water extract showed the highest DPPH activity with IC50 value of 266.02 μg/mL, while the methanol extract showed the highest ABTS activity with IC50 value of 46.49 μg/mL. Futhermore, the n-hexane extract showed activity against Pseudomonas aeruginosa about 5.03% while the water extract showed activity against Bacillus subtilis about 11.65%. This study showed that A. comosus peel has potency as antioxidant and antibacterial agents.
References
Bamidele, P. O. & Fasogbon, B. M., 2017. Chemical and antioxidant properties of snake tomato (Trichosanthes cucumerina) juice and Pineapple (Ananas comosus) juice blends and their changes during storage. Food Chemistry, Volume 220, pp. 184-189.
Brand-Williams, W., Cuvelier, E. M. & Berset, C., 1995. Use of a Free Radical Method to Evaluate Antioxidant Activity. Lebensm. Wiss. u.-Technol, Volume 28, pp. 25-30.
Dutta, S. & Bhattacharyya, D., 2013. Enzymatic, antimycrobial and toxicity studies of the aqueous extract of Ananas comosus (pineapple) crown leaf. Journal of Ethnopharmacology, Volume 150, pp. 451-457.
Gliszczynska-Swiglo, A., 2006. Antioxidant activity of water soluble vitamins in the TEAC (trolox equivalent antioxidant capacity) and the FRAP (ferric reducing antioxidant power) assays. Food Chemistry, Volume 96, pp. 131-136.
Hassan, A. & Othman, Z., 2011. Pineapple (Ananas comosus L. Merr). Kamphang Saen: Woodhead Publishing.
Hatam, F. S., Suryanto, E. & Abidjulu, J., 2013. Aktivitas Antioksidan Dari Ekstrak Kulit Nanas (Ananas comosus (L) Merr). Pharmacon, 2(1).
Hidayati, D. M., Ersam, T., Shimizu, K. & Fatmawati, S., 2017. Antioxidant Activity of Syzygium polyanthum Extracts. Indonesian Journal Chemistry, 17(1), pp. 49-53.
Jiang, L., 2011. Comparison of disk diffusion, agar dillution, and broth microdillution for antimicrobial susceptibility testing of five chitosans. s.l.:Thesis.
Kalpana, B. M., Prasath, S. G. & Subramanian, S., 2014. Studies on the antidiabetic activity of Ananas comosus leaves in STZ induced diabetic rats. Der Pharmacia Lettre, 6(1), pp. 190-198.
Kataki, S. M., 2010. Antibacterial Activity, in vitro Antioxidant Activity And Anthelmintic Activity of Ethanolic Extract of Ananas Comosus L. Tender Leves. Pharmacologyonline, Volume 2, pp. 308-319.
Lawal, D., 2013. Medicinal, Pharmacological and Phytochemical Potentials of Annona Comosus Linn. Peel- A Review. Bayero Journal of Pure and Applied Sciences, 6(1), pp. 101-104.
Lu, X.-H.et al., 2014. Physio-Chemical Properties, Antioxidant Activity and Mineral Contents of Pineapple Genotypes Grown in China. Molecules, Volume 19, pp. 8518-8532.
Rahman, M., 2015. Antimicrobial Secondary Metabolites-Extraction, Isolation, Identification and Bioassay. London: Elsevier Inc..
Re, R. et al., 1999. Antioxidant Activity Applying An Improved ABTS Radical Cation Decolorization Assay. Free Radical Biology and Medicine, 26(9), pp. 1231-1237.
Shalaby, E. A. & Shanab, M., 2013. Antioxidant compounds, assays of determination and mode of action. African Journal of Pharmacy and Pharmacology, pp. 528-539.
Tochi, N. B., Wang, Z., Xu, S.-Y. & Zhang, W., 2008. Therapeutic Application of Pineapple Protease (Bromelin): A Review. Pakistan Journal of Nutrition, 7(4), pp. 513-520.
WHO, 2003. Traditional Medicine, s.l.: Fifty-sixty world health assembly.
