Analysis Of The Enantiomers Ratio Of Citronellal From Indonesian Citronella Oil Using Enantioselective Gas Chromatography

Edy Cahyono, Harno Dwi Pranowo, Muchalal Muchalal, Triyono Triyono


Citronellal 97,3% has been isolated from Java citronella oil (Cymbopogon winterianus) from Yogyakarta Indonesia by fractional distillation under reduced pressure (5 cmHg, 110-120 oC). Citronellal has two optical isomerics that can be separated by capillary column of chiral GC phase. Enantioselective capillary GC with heptakis(2,3-di-O-acetyl-6-O-tert-butyldimethylsilyl)-β-cyclodextrin (β-DEX-225) as stationary phase has been used for analysis of the enantiomers ratio of citronellal. The analysis of enantiomer ratio showed that citronellal contain of 88.21% ee of (R)-(+)-citronellal. Physical properties of isolated citronellal showed that the compound was (+) enantiomer. Structure identification of citronellal was carried out by GC-MS, IR, and 1H NMR, resulted identical fragment and spectra with standard citronellal. Theoretical study with semiempirical-AM1 method showed that energy of (R)-(+)-citronellal on the β-DEX 225 was lower than its (S)-(-)-citronellal.


(R)-(+)-Citronellal; Fractional distillation; β-DEX 225; Enantioselective Gas Chromatography;

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E. Cahyono, Muchalal, Triyono, and H.D. Pranowo, Eksakta Jurnal Ilmu-ilmu MIPA, 2,2 (2010) 79-85.

T.T. Nhu-Trang, H. Casabianca, and M.F. Greiner-Loustalot, Anal Bioanal Chem, 386 (2006) 2141–2152

P.M. Arvela, N. Kumar, V. Nieminen, R. Sjöholm, T. Salmi, and D.Y. Murzin,. Journal of Catalysis, 225 (2004) 155–169

P. Kreis and A. Mosandl, Flav Fragr J, 9 (1994) 249-256.

A. Akhila, Phytochemistry, 25, 2(1986) 421-424

M. Chaplin, Water Structure and Sciences, [27th Oct 2008]

M, J. Lmmerhofer, Chromatograph. A., 1269 (2012)1-2.

HF Kasai, M Tsubuki, Y. Matsumoto , M. Shirao , K. Takahashi, T. Honda , H. Ueda, Chem Pharm Bull (Tokyo). 52 (2004) 311-315.

D.W. Armstrong, T.J. Ward, R.D. Armstrong, T.E. Science. 30, 232 (1986) 1132-1135.



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