Plant growth regulator eEffect on adventitious roots induction of labisia pumila


  • Nor’Aishah Hasan
  • Sobri Hussein
  • Rusli Ibrahim



Adventitious roots, Auxin, Labisia pumila, Plant Growth Regulators, Secondary metabolites


Labisia pumila or locally known as ‘Kacip Fatimah’ is an herbaceous plant commonly used for dysentery, flatulence, dysmenorrhoea and gonorrhoea. A study was undertaken to investigate the effect of different plant growth regulators at various concentrations on adventitious root induction from the leaf explants of Labisia pumila. Murashige and Skoog (MS) medium supplemented with auxins; indole-acetic acid (IAA), indole-butyric acid (IBA) and naphtaleneacetic acid (NAA) each at the concentrations of 0, 1, 3, 5, and 7 mg/L were evaluated for their effects on adventitious root induction. No adventitious roots formed in the control medium devoid of any PGRs. Among the plant growth regulators (PGRs) used, IBA showed the best adventitious roots formation for all explants, followed by NAA and IAA. Leaf explants cultured on MS medium supplemented with 5 mg/L of IBA showed the best rooting ability. In this treatment, 72.4 ± 9.3 % of rooting, 17.8 ± 9.4 roots per explants and 0.123 ± 0.096g of dry weight were recorded. NAA and IAA showed ability to induce adventitious roots from leaf however the rooting ability was relatively lower that IBA treatment. The highest rooting ability for NAA and IAA was achieved in 7 mg/L and 3 mg/L with (71.2 ±10.9 % and 10.6 ± 3.6%) of rooting, (14.9 ± 0.669 and 1.60 ± 0.179) roots per explants and (0.044 ± 0.002g and 0.014 ± 0.001g) of dry weight, respectively.


S. Ercisli, O. Anpali, A. Esitken, A. and U. Sahin, Gartenbauwissenschaft, 67 (2002) 34.

A. J. Jamia, and P. J. Houghton, Seminar on Natural Products, (2000) 118.

K. Karin, H. Maria, and L. Desana, Plant Growth Regulation, 46 (2005) 1.

S. Krisantini, M. Johnston, R.R. William, and C. Beveridge, Scientia Horticulturae, 107 (2006) 171.

A. P. K. Ling, M. F. Chin, and S. Hussein, Plant Science and Biotechnology, 3 (2009) 36.

H. N. Murthy, E. J. Hanh, and K. Y. Paek, Chinese Journal of Biotechnology, 24 (2008) 711.

A. Qaddoury and M. Amssa, Botanical Bulletin of Academia Sinica, 45 (2004) 127.

Z. Rozihawati, H. Aminah, and N. Lokman, Malaysian Science (2003) 112.

K. Terasaka, J. J. Blakeslee, B. Titapiwatanakun, W. A. Peer, A. Bandyopadhyay, and S. N. Makam, Plant Cell, 17 (2005) 2922.

T. Thorpe, C. Stasolla, E. C. Yeung, G. J. De Klerk, A. Roberts, and E. F. George, Plant Propagation by Tissue Culture, Springer, Dordrecht, 2008.

B. K. Zolman, A. Yoder, and B. Bartel, Genetics, 156 (2000) 1323.