Nitric oxide (NO) radical inhibitory of hedyotis philippinensis and its marker compound, asperuloside


  • Emi Norzehan Mohamad Mahbob
  • Rohaya Ahmad
  • Syahida Ahmad



Hedyotis philippinensis, Asperuloside, Anti-inflammatory, Nitric oxide, Lipopolysaccharide (LPS), Interferon- (IFN-


Hedyotis spesies have been used in Chinese folk medicine as a treatment for colds, stomatitis and various inflammations. In this study, three methanolic extracts of Hedyotis species (Hedyotis corymbosa, Hedyotis havilandii and Hedyotis philippinensis) were screened for their anti-inflammatory activity. The plant extracts along with asperuloside, the marker compound of H. philippinensis were tested for their anti-inflammatory effect against lipopolysaccharide (LPS) and interferon- (IFN-)-induced nitric oxide (NO) production using RAW264.7 macrophage cells. Among the three species, H. philippinensis (leaves and stems) showed good NO radical inhibitory activity (with IC50 values of 139.76±12.50 and 176.21±2.48 g/ml, respectively). Asperuloside, isolated as a major compound from the plant moderately inhibited LPS/IFN--induced NO production by 63% at a concentration of 100 M (45% cell viability) compared to L-NAME (77 %). Its IC50 value was found to be 75.45±2.25 M. Its inhibition was also found to be dose-dependent.


J-N. Peng, X-Z. Feng, and X-T. Liang, Phytochemistry, 47 (1998) 1657.

Y. Vodovots, G. Constantine, J. Rubin, M. Csete, E. O. Voit, and G. An, Mathematical Biosciences, 217 (2009) 1.

D. A. Israf, T. A. Khaizurin, A. Syahida, N. H. Lajis, and S. Khozirah, Molecular Immunology, 44 (2007) 673.

A. Panossian, and G. Wikman, Journal of Ethnopharmacology, 118 (2008) 183.

Q-Y. Lin, L-J. Jin, Z-H. Cao, H-Q. Li, and Y-P. Xu, Journal of Ethnopharmacology, 118 (2008) 495.

S. Tewtrakul, C. Wattanapiromsakul, and W. Mahabusarakam, Journal of Ethnopharmacology, 121 (2009) 379.

R. Ahmad, A. M. Ali, D. A. Israf, N. H. Ismail, K. Shaari, and N. H. Lajis, Life Sciences, 76 (2005) 1953.

K. Saha, N. H. Lajis, D. A. Israf, A. S. Hamzah, S. Khozirah, S. Khamis, and A. Syahida, Journal of Ethnopharmacology, 92 (2004) 263.

C. Li., X. Xue, D. Zhou, F. Zhang, Q. Xu, L. Ren, and X. Liang, Journal of Pharmaceutical and Biomedical Analysis, 48 (2008) 205.

B. Li, D-M. Zhang, Y-M. Luo, and X-G. Chen, Chemical & Pharmaceutical Bulletin, 54 (2006) 297.

S-K. Ling, T. Tanaka, and I. Kouno, Biological & Pharmaceutical Bulletin, 26 (2002) 352.

K. Chervenkova, and B. Nikolova-Damyanova, Journal of Liquid Chromatography and Related Technologies, 23 (2000) 741-753.

M. D. C. Recio, R. M. Giner, S. Manez, and J. L. Rios, Planta Medica, 60 (1994) 232.

F. Abas, N. H. Lajis, D. A. Israf, S. Khozirah, and Y. Umi Kalsom, Food Chemistry, 95 (2006) 566.

S. Ahmad, D. A. Israf, N. H. Lajis, K. Shaari, H. Mohamed, A. A. Wahab, K. T. Ariffin, W. Y. Hoo, N. A. Aziz, A. A. Kadir, M. R. Sulaiman, and M. N. Somchit, European Journal of Pharmacology, 538 (2006) 188.

K. S. Kim, H. I. Rhee, E. K. Park, K. Jung, H. J. Jeon, J-H. Kim, H. Yoo, C-K. Han, Y-B. Cho, C. J. Ryu, H. I. Yang, M.C. Yoo, Chinese Medicine, 3 (2008) 3.

W. Zhu, M. Pang, L. Dong, X. Huang, S. Wang, and L. Zhou, Life Sciences, 91 (2012) 369.

M. Liu, L. Zhou, and C. Hu, Pharmacology, Biochemistry and Behavior, 102 (2012) 465.

N. H. Lajis, and R. Ahmad, Studies in Natural Product Chemistry, Bioactive Natural Products (Part M), 33 (2006) 1057.