Brucea javanica seeds as source of potential natural antioxidants to improve biodiesel thermal and oxidative stability


  • Khalil ullah Hasni ISB, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
  • Zul Ilham Zulkiflee Lubes University of Malaya
  • Jamaludin Mohamad University of Malaya
  • Mahendra Varman University of Malaya



Brucea javanica, Biodiesel, Auto-oxidation, Natural Antioxidants, Total Phenolic Content, Total Flavonoid Content, Rancimat test


Biodiesel is one of the most promising renewable energy derived from biological sources. Biodiesel is clean and renewable biofuel but its oxidative stability is low. To increase oxidative stability of biodiesel, natural antioxidants are considered as potential compounds to be used. The purpose of the  work was to investigate and determine the antioxidation potential of Brucea javanica seeds by applying different antioxidant assays such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), metal chelating and ferric reducing antioxidant power (FRAP). Furthermore, Total phenolic content (TPC) and Total flavonoid content (TFC) tests were done. Among the three extracts of Brucea javanica seeds, ethyl acetate extract showed the highest total phenolic content (98.5 ±0.1mg GAE/g dry extract). The highest total flavonoid content (20 ± 0.4mg QAE/g of dry weight) and DPPH inhibition activity 90%. Ethyl acetate extract also exhibited the highest chelating activity with 59% inhibition in the metal chelating assay. However, the Rancimat showed a maximum of 69hour induction period (IP) in biodiesel when gallic acid is added.

Author Biography

Khalil ullah Hasni, ISB, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia

PhD Scholar at Institute of biological Science, Faculty of Science, UM


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