Total phenolic content and antioxidant activity of pure and formulated extracts of kesum (polygonum minus), bawang putih (allivium sativum), pegaga (centella asiatica), and ulam raja (cosmos caudatus)


  • Nooraini Zakaria Universiti Teknologi Malaysia
  • Sayang Baba Universiti Teknologi Malaysia
  • Ku Nurul Aqmar Ku Bahaudin Universiti Teknologi Malaysia
  • Salehhuddin Hamdana Universiti Teknologi Malaysia



Kesum, bawang putih, ulam raja, pegaga, total phenolic content and DPPH radical scavenging assay,


The use of herbs, spices, vegetables and medicinal plants have been traditionally utilize as the alternate medicinal to treat many of diseases by virtue of their antioxidant actions. Kesum, bawang putih, pegaga and ulam raja were extracted by using juice extractor without additional of solvent. The pure extracts were determined for moisture content and the pure and formulation extracts were analyzed for total phenolic content (TPC) and antioxidant activity (DPPH radical scavenging assay). The yield showed that kesum, bawang putih, pegaga and ulam raja extraction yield at 8.5%, 12%, 22.5% and 24% respectively. The results showed that, there was significant difference (P < 0.05) in total phenolic content and antioxidant activity between pure and formulation extracts. Formulated kesum and bawang putih (1:0:0:1) extract had the highest total phenolic content (1703.59 ± 11 GAE/100mg) followed by kesum pure extract (1388.19 ± 11 GAE/100mg) and bawang putih pure extract (1177.87 ± 138.82 GAE/100mg).  No significant was noted and positive Pearson’s correlations between TPC and DPPH assay (r = 0.293) was observed for all plants extract. The statistical indicated that phenolic compounds were not the main contributor of antioxidant activity in plants. Further, there was no synergistic effect observed for pure and formulation extracts.


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