Destruction Of C7, C9 Perfluorocarboxylic Acids By The Strain Ensifer Adhaerens M1
DOI:
https://doi.org/10.11113/mjfas.v20n6.3655Keywords:
Perfluorocarboxylic acid, Ensifer, biodegradation, transformation.Abstract
The aim of the study was to determine the resistance to high concentrations of fluoride ions in the environment of the strain Ensifer adhaerens M1 isolated from soil at a fire extinguishing agent storage and testing site, to determine the growth kinetics and destruction of C7, C9 perfluorocarboxylic acids, perfluoroheptanoic acid and perfluorononanoic acid, respectively, as the sole sources of carbon and energy. The degradation products of perfluorinated carboxylic acids were identified using LCMS-IT-TOF. It was found that the degradation process occurs in multiple stages leading to the formation of perfluorohexanoic acid and accompanied by the release of fluoride ions. Strain Ensifer adhaerens M1 is resistant to F- up to a concentration of 150 mg/L in the environment. Within 14 days of cultivation, bacteria completely utilized perfluoroheptanoic acid and perfluorononanoic acid. Based on chromatography-mass spectrometry and ion chromatography data, a scheme for the stepwise degradation of the investigated perfluorinated acids involving the enzymes coded by novR and dhlA genes is proposed.
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