Microbiological Quality and Antimicrobial Susceptibility Profile of Isolates from Beverages Sold at Ramadan Bazaar in Kuching, Sarawak

Authors

  • Nurul Azyla Azmi ᵃFaculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia ᶜFood Safety and Quality Laboratory Sarawak, Sarawak State Health Department 93590 Kuching, Sarawak, Malaysia
  • Sharifah Aminah Syed Mohamad ᵃFaculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia ᵇMicrobial Metabolite Laboratory, Atta-Ur-Rahman Institute of Natural Product (AURIns), Level 9, FF3 Building, UiTM Puncak Alam Campus, 42300 Puncak Alam, Selangor, Malaysia
  • Khadijah Mohamad Food Safety and Quality Laboratory Sarawak, Sarawak State Health Department 93590 Kuching, Sarawak, Malaysia
  • Suryani Jemain Food Safety and Quality Laboratory Sarawak, Sarawak State Health Department 93590 Kuching, Sarawak, Malaysia
  • Siti Roha Ab Mutalib Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia
  • Fazdlina Sapee Food Safety and Quality Laboratory Sarawak, Sarawak State Health Department 93590 Kuching, Sarawak, Malaysia

DOI:

https://doi.org/10.11113/mjfas.v21n1.3537

Keywords:

Antimicrobial susceptibility profile, beverages, microbiological quality, Ramadan bazaar, Sarawak.

Abstract

Ramadan marks an entire month of fasting by Muslims and is also famous for its festive food bazaar, where people look forward to indulging in their favourite food and beverages. The microbiological safety of food and drink being sold at Ramadan bazaar is one of the important aspects that should be of concern. Thus, the present study was conducted to investigate the microbial quality specifically to beverages sold at Ramadan Bazaar in Kuching, Sarawak and to determine the antimicrobial susceptibility profile of the isolates. Using standard method, 34 beverage samples were analysed for Aerobic Plate Count, coliform, Escherichia coli, Salmonella spp., and Staphylococcus aureus. All samples were positive for coliform. E. coli, and S. aureus except for Salmonella spp. E. coli isolates were resistant to ampicillin, amoxicillin/clavulanic, cefoxitin and tetracycline, while S. aureus isolates were resistant towards ampicillin and penicillin. Hence, further studies need to assess the main source of bacterial contamination in beverages sold at Ramadan bazaars in terms of hygiene and sanitation of vendors.

References

Abdul-Mutalib, N. A., Syafinaz, A. N., Sakai, K., & Shirai, Y. (2015). An overview of food borne illness and food safety in Malaysia. International Food Research Journal, 22(3), 896–901.

Abd Rahim, N. L., Bakar, N. F. A., & Zulfakar, S. S. (2019). Bacterial contamination in foods sold by street vendors around Chow Kit Area. Asia Pacific Environmental and Occupational Health Journal, 5(2), 23–29.

Afreen, A., Ahmed, Z., Ahmad, H., & Khalid, N. (2019). Estimates and burden of food-borne pathogens in RTE beverages in relation to vending practices. Food Quality and Safety, 3(2), 107–115.

Akusu, O. M., Kiin-Kabari, D. B., & Wemedo, S. A. (2016). Microbiological quality of selected street vended foods in Port Harcourt metropolis, Rivers State, Nigeria. Sky Journal of Food Science, 5(2), 008–011.

Amare, A., Worku, T., Ashagirie, B., Adugna, M., Getanaeh, A., & Dagnew, M. (2019). Bacteriological profile, antimicrobial susceptibility patterns of the isolates among street vended foods and hygienic practice of vendors in Gondar town, Northwest Ethiopia: A cross-sectional study. BMC Microbiology, 19(1), 120.

Association of Analytical Communities (AOAC). (2002a). AOAC® Official Method of Analysis 990.12 Aerobic plate count in foods, dry rehydratable film (PetrifilmTM Aerobic Count Plate) method. AOAC Int. Gaithersburg, MD, USA.

Association of Analytical Communities (AOAC). (2002b). AOAC® Official Method of Analysis 991. Coliform and Escherichia coli counts in foods, dry rehydratable film (PetrifilmTM E. coli/Coliform Count PlateTM and PetrifilmTM Coliform Count PlateTM) methods. AOAC Int. Gaithersburg, MD, USA.

Association of Analytical Communities (AOAC). (2003). AOAC® Official Method of Analysis 2003.07 Enumeration of Staphylococcus aureus in selected types of processed and prepared foods, (PetrifilmTM Staph Express Count Plate) Method. AOAC Int. Gaithersburg, MD, USA.

Berita Harian. (2022, April). 93 kes keracunan makanan sejak 1 Ramadan. Berita Harian. https://www.bharian.com.my/berita/nasional/2022/04/945787/93-kes-keracunan-makanan-sejak-1-ramadan

Birgen, B. J., Njue, L.G., Kaindi, D. M., Ogutu, F. O., & Owade, J. O. (2020). Determinants of microbial contamination of street-vended chicken products sold in Nairobi County, Kenya. International Journal of Food Science, 20, 8.

Codex Alimentarius Commission. (2017). International food standards - CXC76R-2017 regional code of hygiene practice for street vended foods in Asia. Retrieved August 15, 2022, from https://www.fao.org/fao-who-codexalimentarius/sh-proxy/en/?lnk=1&url=https%253A%252F%252Fworkspace.fao.org%252Fsites%252Fcodex%252FStandards%252FCXC%2B76R-2017%252FCXP_076Re.pdf

Clinical and Laboratory Standards Institute. (2020). Performance standards for antimicrobial susceptibility testing, 30th Clinical and Laboratory Standards Institute (CLSI) supplement. M100.

Edeghor, U., John, G. E., & Origbu, C. (2019). Bacteriological profile of cut fruits sold in Calabar Metropolis. World News of Natural Sciences, 43–55.

European Committee on Antimicrobial Susceptibility Testing (EUCAST). (2020). Staphylococcus aureus. Calibration of zone diameter breakpoints to MIC values and/or resistance mechanisms. Version 7.0. January 2020. Retrieved August 15, 2022, from https://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Disk_criteria/Validation_2020/S._aureus_v_7.0_January_2020.pdf

Food Standards Australia New Zealand (FSANZ). (2022). Compendium of microbiological criteria for food. https://www.foodstandards.gov.au/publications/Documents/Compendium_revised%20March%202022.pdf (Downloaded on 10 June 2022)

Guidelines for the sale and preparation of fresh sugarcane juice. (20). Food Safety and Quality Division, Ministry of Health Malaysia.

International Standardization Organization (ISO). (2002). Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Salmonella spp. ISO Norm 6579: (2002/COR 1:2004). International Standardization Organization, Geneva, Switzerland.

Jahan, M., Rahaman Sumon, S. M. M., Md. Selim, A. S., & Md. Rahman, M. (2019). Occurrence of antibiotic resistant Escherichia coli and Staphylococcus aureus in street-vended beverages accessible in an industrial zone of Bangladesh. Journal of Food Safety & Hygiene, 5(4), 237–247.

Malaysian Food Hygiene Regulation. (2009). Malaysian Food Act 1983 & Regulations.

Mat Zin, A. A., Aklilu, E., Goriman Khan, M. A. K., Hamdan, R., Imad Ibrahim, A. S., & Soon, J. M. (2017). Microbiological quality of cooked meat products sold in Kelantan, Malaysia during Ramadhan month. International Food Research Journal, 24(1), 4–421.

Mohd Nawawee, N. S., Abu Bakar, N. F., & Zulfakar, S. S. (2019). Microbiological safety of street-vended beverages in Chow Kit, Kuala Lumpur. International Journal of Environmental Research and Public Health, 16(22), 4463.

Nik Rosmawati, N. H., Wan Manan, W. M., Noor Izani, N. J., & Nik Nurain, N. H. (2015). Validity and reliability of food safety knowledge and practices questionnaire among food handlers. Health and the Environmental Journal, 6(1), 11–30.

Oliveira, J. D. S. C., & Josée, J. F. B. D. S. (2019). Food handling practices and microbial quality in street food. Journal of Food and Nutrition Research, 7(4), 319–324.

Paul, P., Amin, M. R., Ahad, A., Meher, M. M., Rume, F. I., Jahan, S., & Anower, M. (2018). Bacteriological contamination of street food vended in Chittagong, Bangladesh. Annals of Life Sciences, 1, 15–22.

Raza, J., Asmat, T. M., Mustafa, M. Z., Ishtiaq, H., Mumtaz, K., Jalees, M. M., Samad, A., Shah, A. A., Khalid, S., & Rehman, H. (2021). Contamination of ready-to-eat street food in Pakistan with Salmonella spp: Implications for consumers and food safety. International Journal of Infectious Diseases, 106, 123–127.

Rezaeigolestani, M., Hashemi, M., & Nematy, M. (2017). Risk factors of food-borne bacterial diseases in Ramadan. Journal of Fasting & Health, 5(4), 138–3.

Sabuj, A. A. M., Haque, Z. F., Barua, N., Islam, M. A., & Saha, S. (2018). Assessment of bacteriological quality of street vended fast foods and their antimicrobial resistance. International Journal of Current Microbiology and Applied Sciences, 7(11), 3049–3059.

Sharma, N., Singh, K., Toor, D., Pai, S. S., Chakraborty, R., & Khan, M. (2020). Antibiotic resistance in microbes from street fruit drinks and hygiene behavior of the vendors in Delhi, India. International Journal of Environmental Research & Public Health, 17, 4829.

Shrimali, V. V., & Shah, K. K. (2017). A microbial study of water used by street food vendors and microbial flora found on their hands, in a densely populated urban area of Vadodara, Gujarat. Journal of Integrated Health Sciences, 5(2), 81–86.

Suara Sarawak. (2023, April 1). 10 notis dikeluarkan terhadap peniaga, pengendali makanan bazar Ramadan sekitar Kuching. https://suarasarawak.my/10-notis-dikeluarkan-terhadap-peniaga-pengendali-makanan-bazar-ramadan-sekitar-kuching/#google_vignette

The Star. (2022, May 6). Food poisoning: 10 clusters detected in Sabah during fasting month, only two linked to bazaars. https://www.thestar.com.my/news/nation/2022/05/06/food-poisoning-10-clusters-detected-in-sabah-during-fasting-month-only-two-linked-to-bazaars

Thi, A. N. T., Kittirath, P., Abiola, S. D., Duy, L. N. D., & Ha, N. C. (2021). Evaluation of street food safety and hygiene practices of food vendors in Can Tho City of Vietnam. Current Research in Nutrition and Food Science, 9(1), 158–171.

Thongkaow, P., Kummuda, A., Sawee, S., & Makkaew, P. (2021). Bacteriological quality of ice and associated sanitary conditions in food and beverages premises in Thasala, Nakhon Si Thammarat, Thailand. Malaysian Journal of Public Health Medicine, 21(2), 252–256.

Uddin, M. E., Akter, T., Parvez, M. A. K., Nahar, S., Pervin, S., Debnath, B., & Datta, S. (2017). Microbial safety of street vended fruit juices in Dhaka City of Bangladesh. Journal of Advances in Microbiology, 3(2), 1–7.

Upadhyaya, S., Srivastava, P., Chandra, R., & Arora, N. (2017). Microbiological assessment and hazardous effects of ready-to-eat foods presented for sale in Lucknow City, India. African Journal of Food Science, 11(10), 346–352.

Utusan Borneo. (2023a, April 1). 38 kompaun dikeluarkan dalam Ops Bazar Ramadan 2023 di Miri. https://www.utusanborneo.com.my/2023/04/01/38-kompaun-dikeluarkan-dalam-ops-bazar-ramadan-2023-di-miri

Utusan Borneo. (2023b, October 24). Peniaga didenda RM1,000 kerana jual makanan tercemar. https://www.utusanborneo.com.my/2023/10/24/peniaga-didenda-rm1000-kerana-jual-makanan-tercemar?fbclid=IwAR3guX-oahYOZLGFEykh3NNrC6I15fxPYmWut6i2n-1PXynJHtNLlDg-NQo

World Health Organization (WHO). (1996). Essential safety requirements for the street vended foods (No. WHO/FNU/FOS/96.7 Rev1). Food Safety Unit, Division of Food and Nutrition, World Health Organization, Geneva.

Yeleliere, E., Cobbina, S. J., & Abubakari, Z. I. (2017). Review of microbial food contamination and food hygiene in selected capital cities of Ghana. Cogent Food & Agriculture, 3(1), 1395102.

Zulfakar, S. S., Zin, N. M., Zalami, S. N. S. M., & Nawawee, N. S. M. (2021). Identification and antibiotic resistance profile of Salmonella spp. and Citrobacter spp. isolated from street-vended beverages. Pertanika Journal of Science & Technology, 29(1), 593–606.

Downloads

Published

21-02-2025