Cd2+ Sequestration by Ureolytic Bacteria Isolated from Goat Faeces and Nursery Wastewater
DOI:
https://doi.org/10.11113/mjfas.v20n5.3425Keywords:
Heavy metal, bioremediation, ureolytic bacteria, MICP, wastewater.Abstract
As modern industrial production makes extensive use of heavy metals, a significant amount of sewage and solid wastes containing heavy metals are released into the environment. There is significant potential for bioremediation of multiple heavy metal contamination using biomineralization to immobilise the toxic metal. In this study, microbially induced carbonate precipitation (MICP) technology was used to treat Cd2+ contamination on plants, humans, and the environment. Ureolytic bacteria that collected from goat faeces and nursery wastewater were stimulated using enrichment technique. Next, physicochemical properties of the bacteria including urea agar base test, biomineralization test, conductivity & pH measurement test and optical density test were examined through enrichment subculturing of the bacteria. The morphological characterization of precipitate formed from both samples were analayzed by using scanning electron microscopy- energy dispersive x-ray diffraction method. Ureolytic bacteria of the goat faeces sample demonstrated greater tolerance to Cd2+ concentration compared to the nursery wastewater sample by obtaining a higher optical density values and larger amounts of precipitate produced by the goat faeces sample across all tested Cd2+ concentrations. As a result, from the atomic absorption spectrophotometry (AAS) analysis, the goat faeces sample showed higher efficiency in removing Cd2+ compared to the nursery wastewater sample, with a removal efficiency of 89.06% for the goat faeces sample and 77.75% for the nursery wastewater sample. Hence, this study confirmed that the interaction between ureolytic bacteria isolated from waste sources and heavy metal ions is vital for the overall effectiveness of heavy metal removal.
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Copyright (c) 2024 Muhammad Daniel Hamdan, Hazlami Fikri Basri, Armstrong Ighodalo Omoregie, Mohd Akmali Mokhter, Koji Iwamoto, Tariq Ouahbi
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