Spectrophotometric Determination and Biological Activity Study of Zn(II) using a Newly Synthesized Azo Reagent Derivative from 4,5-bis(4-methoxyphenyl) Imidazole

Esraa Rasool Radhi

doi:10.11113/mjfas.v20n1.3251

Authors

Esraa Rasool Radhi Ministry of Education, Kufa, Iraq

DOI:

https://doi.org/10.11113/mjfas.v20n1.3251

Keywords:

Spectrophotometric determination, Imidazole derivative, Synthesized azo reagent, Zn(II)-azo complex, Biological activity

Abstract

A rapid and simple spectrophotometric method was submitted for Zn(II) determination using (E)-2-(4,5-bis(4-methoxyphenyl)-1H-imidazole-4-yl) diazenyl) benzoic acid as an analytical reagent. The suggested method is based on the chelation reaction between Zn(II) and the bidentate chelation ligand with a mole ratio of 1:2 (metal:ligand) with octahedral geometry. The maximum absorption of the formed violet complex was 560 nm, the linearity was in the concentration range of 0.5-15.0 µg mL^-1. The relative standard deviation (n =10) was 0.149%. The interfering effect of diverse cations and anions was tested. To clarify the biological efficacy of the synthesized Zn(II) complex, an in vitro biological activity study was carried out against four kinds of bacterial strains, and the results showed promising inhibition activity.

References

Mohammed, H.S. (2020). Synthesis, characterization, structure determination from powder X-ray diffraction data, and biological activity of azo dye of 3-aminopyridine and its complexes of Ni (II) and Cu (II). Bull. Chem. Soc. Ethiop., 34(3), 523-532.

Hussain, K. & Naser, A.N. (2023). A green product using selective compound for susceptible assessment of copper in blood serum. Current Applied Science and Technology, 23(4), 10-55003.

Jumana, W. A., Zainab, A. K., Marwa N. G., & Naser A. N. (2021).

Synthesis of a new organic probe 4-(4 acetamidophenylazo) pyrogallol for spectrophotometric determination of Bi(III) and Al(III) in pharmaceutical samples.

Rev. Anal. Chem., 40(1), 108-126.

Naser A. N., Kasim M. A., Zainab A. K. (2018). New approach for determination of sulfadiazine in pharmaceutical preparations using 4(4-sulphophenylazo)pyrogallol: Kinetic spectrophotometric method. Spectrochim. Acta, Part A, 201, 267-280.

MOHAMMED, A., & BAQIR, S.J. (2015). Spectrophotometric determination of zinc in pharmaceutical preparations by using a new synthesized reagent [4, 5-diphenyl-2-((1E)-(4-(1-(2-phenylhydrazono) ethyl) phenyl) diazenyl)-4H-imidazole]. Iraqi Natl. J. Chem., 15(3), 271-283.

Tunçel, M., & Serin, S. (2006). Synthesis and characterization of new azo-linked Schiff bases and their cobalt (II), copper (II) and nickel (II) complexes. Transition metal chemistry, 31(6), 805-812.

Rollas, S., & Güniz Küçükgüzel, Ş. (2007). Biological activities of hydrazone derivatives. Molecules, 12(8), 1910-1939.

Karipcin, F., & Kabalcilar, E. (2007). Spectroscopic and thermal studies on solid complexes of 4-(2-pyridylazo) resorcinol with some transition metals. Acta Chim. Slov., 54(2), 242-247.

Kirem, H., & Naser, N. A. (2023). A green product using selective compound for susceptible assessment of copper in blood serum. Current Applied Science and Technology, 10-55003.

Aljamali, N. M., & Hassen, H. S. (2021). Review on azo-compounds and their applications. Journal of Catalyst & Catalysis, 8(2), 8-16.

Adnan, S. (2020). Synthesis, spectral characterization and anticancer studies of novel azo schiff base and its complexes with Ag (I), Au (lll) And Pt (lV) ions. Egypt. J. Chem., 63(12), 4749-4756.

Karam, F. (2022). Synthesis, identification, biological activity and anti-cancer activity Studies of Hetrocyclic Ligand Azo-schiff Base with Au (III) Complex. Egypt. J. Chem., 65(1), 327-334.

Shihad, A. A. A., Hessoon, H. M., Karam, F. F., & Al-Adilee, K. J. (2021). Novel method for determination of Zinc in some pharmaceutics using new prepared reagent of methyl phenol. In IOP Conference Series: Earth and Environmental Science, 790(1), 012010.

Jassim, T. J., Hessoon, H. M., & Karam, F. F. (2023). Preparation and characterization of new hetrocyclic azo thiozal dye ligand and its use as a reagent for determination of Zn+2 ion in drug by new analytical method. J. Med. Chem. Sci., 6(4), 857-867.

Moustafa, A. H., El-Sayed, H. A., Amin, A. S., El Haggar, A. M., & Gouda, A. A. (2023). Efficiency enhancement of the spectrophotometric estimation of zinc in water, food, tobacco and pharmaceutical preparations samples utilizing cloud point extraction. Egyptian Journal of Chemistry, 66(7), 553-562.

Jomova, K., & Valko, M. (2011). Advances in metal-induced oxidative stress and human disease. Toxicology, 283(2-3), 65-87.

Haase, H., & Rink, L. (2014). Zinc signals and immune function. Biofactors, 40(1), 27-40.

Chasapis, C. T, Ntoupa, P-SA, Spiliopoulou, C. A., & Stefanidou, M. E. (2020). Recent aspects of the effects of zinc on human health. Arch. Toxicol., 94(5), 1443-60.

Jaffer, N. D. (2019). Cloud-point technique for extraction and spectral estimation of ZINC (II) in different samples. Biochem. Cell. Arch., 19(2), 4683-4690.

Alabidi, H. M., Farhan, A. M., & Al-Rufaie, M. M. (2021). Spectrophotometric determination of Zn (II) in pharmaceutical formulation using a new azo reagent as derivative of 2-Naphthol. Current Applied Science and Technology, 21(1), 176-187.

Tufekci, M., Bulut, V.N., Elvan, H., Ozdes, D., Soylak, M., & Duran, C. (2013). Determination of Pb (II), Zn (II), Cd (II), and Co (II) ions by flame atomic absorption spectrometry in food and water samples after preconcentration by coprecipitation with Mo (VI)-diethyldithiocarbamate. Environ. Monit. Assess., 185, 1107-1115.

Thanh, N. M., Van Hop, N., Luyen, N. D., Phong, N. H., & Tam Toan, T. T. (2019). Simultaneous determination of Zn (II), Cd (II), Pb (II), and Cu (II) using differential pulse anodic stripping voltammetry at a bismuth film-modified electrode. Adv. Mater. Sci. Eng., 2019.

Xing, G., Sardar, M. R., Lin, B., & Lin, J. M. (2019). Analysis of trace metals in water samples using NOBIAS chelate resins by HPLC and ICP-MS. Talanta, 204, 50-56.

Ding, H., Wang, R., Liu, W., Cheng, S., Ji, W., Zheng, Z., & Wang, X. (2019). A spherical metal-organic coordination polymer for the microextraction of neonicotinoid insecticides prior to their determination by HPLC. Microchim. Acta, 186, 1-8.

Shamsipur, M., Zahedi, M.M., De Filippo, G., & Lippolis, V. (2011). Development of a novel flow injection liquid–liquid microextraction method for the on-line separation and preconcentration for determination of zinc (II) using 5-(8-hydroxy-2-quinolinylmethyl)-2, 8-dithia-5-aza-2, 6-pyridinophane as a sensitive and selective fluorescent chemosensor. Talanta, 85(1), 687-693.

Gaubeur, I., Ávila-Terra, L. H. S., Masini, J. C., & Suárez-Iha, M. E. V. (2007). Spectrophotometric flow injection methods for zinc determination in pharmaceutical and biological samples. Anal. Sci., 23(10), 1227-1231.

Abd, Z.G., & Ali, A.M. (2020). Preparation and identification of some new mixed ligand complexes containing imidazole and 1, 10-phenanthroline compounds. Journal of Kufa for Chemical Science, 2(6).

Ali, F.J., Radhi, E. R., & Ali, K. J. (2022). Synthesis and characterization of a new azo ligand derivative from 4, 5-BIS (4-Methoxyphenyl) imidazol and its metal complexes and biological activity study of its PD (II) Complex. Pakistan Journal of Medical & Health Sciences, 16(07), 431-431.

Radhi, E. R. (2022). Spectrophotometric determination of Hg (II) using (E)-2-(4, 5-bis (4-methoxyphenyl)-1H-imidazol-4-yl) diazenyl) benzoic acid as analytical reagent. J. Pharm. Negat. Results, 13(SI 1), 530-536.