Development of Synthetic Lumbar Vertebrae using Custom-made Mould for Orthopaedics Training: Micro-Structural Analysis

Nurul Najihah Ahmad Zamri; Siti Adawiyah Zulkefli; Nik Nur Ain Azrin Abdullah; Nur Syafiqah Faidzul Hassan; Ahmad Kafrawi Nasution; Muhammad Hanif Ramlee

doi:10.11113/mjfas.v20n3.3157

Authors

Nurul Najihah Ahmad Zamri Bone Biomechanics Laboratory (BBL), Department of Biomedical Engineering and Health Sciences, Faculty of Electrical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
Siti Adawiyah Zulkefli Bone Biomechanics Laboratory (BBL), Department of Biomedical Engineering and Health Sciences, Faculty of Electrical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
Nik Nur Ain Azrin Abdullah Bone Biomechanics Laboratory (BBL), Department of Biomedical Engineering and Health Sciences, Faculty of Electrical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
Nur Syafiqah Faidzul Hassan Bone Biomechanics Laboratory (BBL), Department of Biomedical Engineering and Health Sciences, Faculty of Electrical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
Ahmad Kafrawi Nasution Department of Mechanical Engineering, Faculty of Engineering, Muhammadiyah University of Riau, Pekanbaru, 28291 Riau, Indonesia
Muhammad Hanif Ramlee ᵃBone Biomechanics Laboratory (BBL), Department of Biomedical Engineering and Health Sciences, Faculty of Electrical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia; ᶜBioinspired Device and Tissue Engineering (BIOINSPIRA) Research Group, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia

DOI:

https://doi.org/10.11113/mjfas.v20n3.3157

Keywords:

Mould, Lumbar, Cortical, Cancellous, Pore, Polyurethane

Abstract

Human vertebrae composed of cortical and cancellous bone are crucial to provide the structural support of human framework and protect the organs underneath. Vertebrae from the Lumbar 3 (L3) was selected for fabrication because it is the most commonly used for surgical training. The use of human vertebrae in the orthopaedic studies is necessary to investigate various complications such as vertebral compression fracture and to propose the most suitable medical intervention for the treatment. However, investigations of cortical and cancellous material properties and composition have been limited. Therefore, the main objective of this study was to design a custom-made mould for synthetic lumbar vertebrae using moulding techniques that could exhibit the same anatomical structure as real vertebrae. Then, this study was carried out to establish a process of cortical and cancellous synthetic bone development. Lastly, this study was done to compare the morphological properties of synthetic bone produced with human bone. Utilizing Polyurethane (PU) as the main material was used for the fabrication, where it could inhibit the morphological properties that could mimic the human bone. The methods to fabricate the vertebrae also varied from static mould and rotational mould. The fabricated lumbar produced was tested for the morphological properties, targeting the pore diameter, was performed using Scanning Electron Microscopy (SEM). From this study, it was identified that the analysis of pore diameter from specimen of Ratio 3 from rotational mould depicts the smallest pore diameter and standard deviation, 1.5 ± 0.3 mm. While this specimen has the smallest value, the results were still higher when compared to the pore diameter of human trabecular bone (which ranges from 0.3 mm to 0.6 mm). In conclusion, the use of silicone custom-made moulds could provide synthetic vertebrae with an anatomical structure mimicking real human vertebrae.

References

Abd Aziz, A. U., Ammarullah, M. I., Bing Wui, N., Hong-Seng, G., Abdul Kadir, M. R., Ramlee, M. H. (2024). Unilateral external fixator and its biomechanical effects in treating different types of femoral fracture: A finite element study with experimental validated model. Heliyon, 10, e26660.

Barba, D., Alabort, E., & Reed, R. C. (2019). Synthetic bone: Design by additive manufacturing. Acta biomaterialia, 97, 637-656.

Farhana, S. M. A., Mohd Haziq, D., Nurfatmah Pz, N., & Majid, R. A. (2014). Effects of silicone surfactant on the water absorption and surface morphology of rigid palm oil-based polyurethane foam. Applied Mechanics and Materials, 554, 185-188.

Frost, B. A., Camarero-Espinosa, S., & Foster, E. J. (2019). Materials for the spine: anatomy, problems, and solutions. Materials, 12(2), 253.

Han, M. S., Choi, S. J., Kim, J. M., Kim, Y. H., Kim, W. N., Lee, H. S., & Sung, J. Y. (2009). Effects of silicone surfactant on the cell size and thermal conductivity of rigid polyurethane foams by environmentally friendly blowing agents. Macromolecular Research, 17, 44-50.

Hollensteiner, M., Botzenmayer, M., Fürst, D., Winkler, M., Augat, P., Sandriesser, S., ... & Schrempf, A. (2018). Characterization of polyurethane-based synthetic vertebrae for spinal cement augmentation training. Journal of Materials Science: Materials in Medicine, 29, 1-11.

Hopkins, W. G. (2017). Spreadsheets for analysis of validity and reliability. Sportscience, 21.

Modi, Y. K, & Sanadhya, S. (2018). Design and additive manufacturing of patient-specific cranial and pelvic bone implants from computed tomography data. Journal of the Brazilian Society of Mechanical Sciences and Engineering, 40(10), 1-11.

Muhayudin, N. A., Basaruddin, K. S., McEvoy, F., & Tansey, A. (2020). Evaluating compressive properties and morphology of expandable polyurethane foam for use in a synthetic paediatric spine. Journal of Materials Research and Technology, 9(2), 2590-2597.

Shim, V., Boheme, J., Josten, C., & Anderson, I. (2018). Use of polyurethane foam in orthopaedic biomechanical experimentation and simulation. Polyurethane, 171-200.

Ramlee, M. H., Gan, H. S., Daud, S. A., Abdul Wahab, A., Abdul Kadir, M. R. (2020). Stress distributions and micromovement of fragment bone of pilon fracture treated with external fixator: A finite element analysis. The Journal of Foot and Ankle Surgery, 59(4), 664-472.

Zulkefli, S. A., Shaikhani, M. H., Nasution, A. K., Abdul Wahab, A., Abdul Kadir, M. R., Ramlee, M. H. (2019). Fabrication and biomechanical evaluation of polyurethane material for synthetic bone. Journal of Physics: Conference Series, 1372(1), 012013.

Zulkefli, S.A., Shaikhani, M. H., Nasution, A. K., Hong-Seng, G., Ramlee, M. H. (2019). Polyurethane material for synthetic tibia bone application. Materials: Technology and Application, 20-36.

Zainal Abidin, N. A., Abdul Kadir, M. R., Ramlee, M. H. (2019). Three dimensional finite element modelling and analysis of human knee joint- model verification. Journal of Physics: Conference Series, 1372(1), 012068.