Identification and Antimicrobial Activity of Lactic Acid Bacteria Isolated from Tualang Bee Honey
DOI:
https://doi.org/10.11113/mjfas.v19n4.2956Keywords:
Tualang Honey, LAB, pathogen, 16s rRNA sequencing, antimicrobial activityAbstract
Malaysia tualang honey usually collected from the combs of Asia rock bees (Apis dorsata), which build their hives high up in the tualang tree (Koompassia excelsa). Tualang honey is used commonly as a medicinal product and as food in Malaysia. LAB strains have been discovered to exhibit antimicrobial properties even though especially in Malaysia, the research on LAB found from honey are still scarce. The study was conducted to isolate and identify lactic acid bacteria (LAB) from Tualang honey bee from East Coast Peninsular Malaysia. The Tualang honey identified LAB strains were further tested against several pathogenic bacteria by using well-diffusion method. The LAB were primarily identified by colony morphology, microscopy of Gram`s stain, biochemical tests and 16s rRNA sequencing method. 10 isolates LAB that has been identified as Leuconostoc mesenteroides (5 isolates), Lactobacillus kunkeei (4 isolates), and Lactobacillus farraginis (1 isolates) were selected for the characterization of antimicrobial agents produced by LAB. It was found that from 10 isolates, 5 isolates exhibited strong antimicrobial activity against Staphylococcus epidermidis and Pseudomonas aeruginosa results in excellent inhibition zones diameters larger than 15 mm. This study indicated that lactic acid bacteria isolated from Tualang honey has potential antimicrobial activity against pathogenic bacteria and can be further characterized for health benefits and potential use in food industry at Malaysia.
Keywords: Tualang Honey, LAB, pathogen, 16s rRNA sequencing, antimicrobial activity.
References
Abdelbasset, M., Manel, D., Taha, M. and Djamila, K. (2014). Enzymatic and functional properties of lactic acid bacteria isolated from Algeria fermented milk products. Advances in Natural and Applied Sciences, 8(8), 141-151.
Adeniyi, B. A., Adetoye, A. and Ayeni, F. A. (2015). Antibacterial activities of lactic acid bacteria isolated from cow faeces against potential enteric pathogens. African Health Sciences, 15(3), 888-895.
Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. and Lipman, D. J. (1997). Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Research, 25(17), 3389-3402.
Ammor, S., Tauveron, G., Dufour, E. and Chevallier, I. (2006). Antibacterial activity of lactic acid bacteria against spoilage and pathogenic bacteria isolated from the same meat small-scale facility: 1—Screening and characterization of the antibacterial compounds. Food Control, 17(6), 454-461.
Aween, M. M., Hassan, Z., Muhialdin, B. J., Noor, H. M. and Eljamel, Y. A. (2012). Evaluation on antibacterial activity of Lactobacillus acidophilus strains isolated from honey. American Journal of Applied Sciences, 9(6), 807.
Aween, M. M., Zaiton, H., and Belal, J. (2010). Antimicrobial activity of lactic acid bacteria isolated from honey. Proceedings of the International Symposium on Lactic Acid Bacteria (ISLAB’10) (pp. 25-27). 28 August - 1 September 2011, University Putra Malaysia.
Babendreier, D., Joller, D., Romeis, J., Bigler, F. and Widmer, F. (2007). Bacterial community structures in honeybee intestines and their response to two insecticidal proteins. FEMS Microbiology Ecology, 59(3), 600-610.
Batista, A. L. D., Silva, R., Cappato, L. P., Ferreira, M. V. S., Nascimento, K. O., Schmiele, M., Esmerino, E. A., Balthazar, C. F., Silva, H. L. A., Moraes, J. and Pimentel, T. C. (2017). Developing a synbiotic fermented milk using probiotic bacteria and organic green banana flour. Journal of Functional Foods, 38, 242-250.
Corsetti, A., Lavermicocca, P., Morea, M., Baruzzi, F., Tosti, N. and Gobbetti, M. (2001). Phenotypic and molecular identification and clustering of lactic acid bacteria and yeasts from wheat (species Triticum durum and Triticum aestivum) sourdoughs of Southern Italy. International Journal of Food Microbiology, 64(1-2), 95-104.
Forsgren, E., Olofsson, T. C., Váasquez, A. and Fries, I. (2010). Novel lactic acid bacteria inhibiting Paenibacillus larvae in honey bee larvae. Apidologie, 41(1), 99-108.
Ibarguren, C., Raya, R. R., Apella, M. C., and Audisio, M. C. (2010). Enterococcus faecium isolated from honey synthesized bacteriocin-like substances active against different Listeria monocytogenes strains. The Journal of Microbiology, 48(1), 44-52.
Lee, H., Churey, J. J. and Worobo, R. W. (2008). Antimicrobial activity of bacterial strains from different floral sources of honey. International Journal of Food Microbiology, 126(1-2), 240-244.
Maragkoudakis, P. A., Mountzouris, K. C., Psyrras, D., Cremonese, S., Fischer, J., Cantor, M. D. and Tsakalidou, E. (2009). Functional properties of novel protective lactic acid bacteria and application in raw chicken meat against Listeria monocytogenes and Salmonella enteritidis. International Journal of Food Microbiology, 130(3), 219-226.
Mojgani, N. A. C. A. and Amirnia, C. (2007). Kinetics of Growth and bacteriocin production in L. casei RN 78 isolated from a dairy sample in IR Iran. International Journal of Dairy Science, 2(1), 1-12.
Nigam, A., Kumar, A., Madhusuda, H. V. and Bhola, N. (2012). In-vitro Screening of antibacterial activity of lactic acid bacteria against common enteric pathogens. Journal of Biomedical Sciences, 1(4).
Olofsson, T. C. and Vásquez, A. (2008). Detection and identification of a novel lactic acid bacterial flora within the honey stomach of the honeybee Apis mellifera. Current Microbiology, 57(4), 356-363.
Parmjit, S. (2011). Fermented dairy products: starter cultures and potential nutritional benefits. Food and Nutrition Sciences, 2011.
Ponce, A. G., Moreira, M. R., Del Valle, C. E. and Roura, S. I. (2008). Preliminary characterization of bacteriocin-like substances from lactic acid bacteria isolated from organic leafy vegetables. LWT-Food Science and Technology, 41(3), 432-441.
Roslan, N. A., Rashed, H. M., Harun, N. H., Jamaludin, M. H., Kari, Z. A. and Alimon, A. R. (2015). The antibacterial activity of diluted Tualang honey. Malaysia Journal of Animal Science, 18(1), 99-104.
Tamang, B., Tamang, J. P., Schillinger, U., Franz, C. M., Gores, M. and Holzapfel, W. H. (2008). Phenotypic and genotypic identification of lactic acid bacteria isolated from ethnic fermented bamboo tender shoots of North East India. International Journal of Food Microbiology, 121(1), 35-40.
Vasquez, A. and Olofsson, T. C. (2009). The lactic acid bacteria involved in the production of bee pollen and bee bread. Journal of Apicultural Research, 48(3), 189-195.
Downloads
Published
Issue
Section
License
Copyright (c) 2023 Amir Izzwan Zamri, Nur Hazwani Mohd Hasali, Fisal Ahmad, Tuan Zainazor Tuan Chilek, Muhammad Nur Shazwan Jamzuri, Zuha Rosufila Abu Hasan, D. N. Shariff
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
