Quality Evaluation of Buffalo Meatballs Produced at Different Comminution Process Temperatures


  • Lim Jwee Yie Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia
  • Nurul Izzah Khalid Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia
  • Mohammad Rashedi Ismail-Fitry ᵃDepartment of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia; ᵇHalal Products Research Institute, Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia




Comminution temperature, buffalo meatballs, meat emulsion, physicochemical, sensory


Buffalo meatballs were formulated and the effects of different comminution temperatures on the quality (cooking yield, water holding capacity (WHC), protein, texture, colour, and sensory) were evaluated. During the mixing of ingredients, the comminution temperature was adjusted using different types of water which were ice (0°C), ice water (4°C), cold water (10°C), room temperature water (22°C), and warm water (32°C). Following comminution for 3 minutes, the temperatures of the batters were recorded at 14, 25, 25, 29, and 27°C, respectively. The comminution took a total of 15 minutes had produced batters with similar final temperatures (ranging from 36 to 38°C), except ice temperature mixing (28°C). Cold water meatballs produced the highest cooking yield but significantly the lowest (P<0.05) water-holding capacity. The colour of the cold water meatball shows significantly (P<0.05) the highest L* (lightness), significantly (P>0.05) the lowest a* (redness), and the lowest b* (yellowness) values. While the textures (hardness, cohesiveness, gumminess, chewiness, and springiness) of all meatballs were similar (P>0.05). All meatball samples had nearly similar soluble protein concentrations (0.97 to 1.06 ug/ml) but ice water meatballs had the highest (P<0.05). The panellists gave all the meatballs a score ranging from 6.32 to 6.98, with ice meatballs receiving the highest mean score (6.98) acceptability score (P>0.05). In conclusion, comminuted buffalo meatballs can be produced using either ice, ice water, cold water, room temperature water, or warm water without affecting their quality. However, ice is suggested for safety purposes against microbial growth during processing.


Department of Statistics Malaysia. (20220. Supply and utilization accounts selected agricultural commodities, Malaysia 2017-2021. https://www.dosm.gov.my/v1/index.php?r=column/cthemeByCat&cat=164&bul_id=MlpTUkxISFB1SFNDQ2pTWTlEOXZkZz09&menu_id=Z0VTZGU1UHBUT1VJMFlpaXRRR0xpdz09.

Maheswarappa, N. B., Muthupalani, M., Mohan, K., Banerjee, R., Sen, A. R., & Barbuddhe, S. B. (2022). Asiatic water buffalo: A sustainable and healthy red meat source. Springer.

Anjaneyulu, A. S. R., Thomas, R., & Kondaiah, N. (2007). Technologies for value added buffalo meat products-A review. American Journal of Food Technology, 2(3), 104-114.

Department of Veterinary Services. (2022). Price of Beef 2022. https://www.dvs.gov.my/dvs/resources/user_1/2022/BPSPV/HARGA/LEMBU_2022.pdf.

Department of Veterinary Services. (2022). Price of Buffalo Meat 2022. https://www.dvs.gov.my/dvs/resources/user_1/2022/BPSPV/HARGA/KERBAU_2022.pdf.

Sebranek, J. G. Basic Curing Ingredients. In R. Tarté (Ed.), Ingredients in Meat Products: Properties, Functionality and Applications (1–24). Springer Science & Business Media.

Thomas, R., Anjaneyulu, A. S. R., Gadekar, Y. P., Pragati, H., & Kondaiah, N. (2007). Effect of comminution temperature on the quality and shelf life of buffalo meat nuggets. Food Chemistry, 103(3), 787-794.

Swift СЕ, L. С., & Fryar, A. J. (1961). Comminuted meat emulsions-the capacity of meats for emulsifying fats. Food Technology, 15, 468-473.

Sutton, D. S., Hand, L. W., & Newkirk, K. A. (1995). Reduced fat, high moisture beef frankfurters as affected by chopping temperature. Journal of Food Science, 60(3), 580-582.

Brown, D. D., & Toledo, R. T. (1975). Relationship between chopping temperatures and fat and water binding in comminuted meat batters. Journal of Food Science, 40(5), 1061-1064.

Brown, S., & Ledward, D. A. (1987). Effect of temperature of comminution on the stability and eating quality of ‘English’ sausages. Meat Science, 20(2), 97-105.

Helmer, R. L., & Saffle, R. L. (1963). Effect of chopping temperature on stability of sausage emulsions. Food Technology, 17(9), 1195.

Jones, K. W., & Mandigo, R. W. (1982). Effects of chopping temperature on the microstructure of meat emulsions. Journal of Food Science, 47(6), 1930-1935.

Webb, N. B., Rao, V. N. M., Howell, A. J., Barbour, B. C., & Monroe, R. J. (1975). Effect of lipid and chopping temperatures on sausage emulsion stability in a model system. Journal of Food Science, 40(6), 1210-1213.

Meng, X., Wu, D., Zhang, Z., Wang, H., Wu, P., Xu, Z., Gao, Z., Mintah, B. K., & Dabbour, M. (2022). An overview of factors affecting the quality of beef meatballs: Processing and preservation. Food Science & Nutrition, 10(6), 1961-1974.

Rifqie Mariana, R., Hidayati, L., & Soekopitojo, S. (2019). Implementing the HACCP system to the production of Bakso Malang-Indonesia. Journal of Culinary Science & Technology, 17(4), 291-312.

Huda, N., Shen, Y. H., Huey, Y. L., Ahmad, R., & Mardiah, A. (2010). Evaluation of physico-chemical properties of Malaysian commercial beef meatballs. American Journal of Food Technology, 5(1), 13-21.

Asyrul-Izhar, A. B., Sarbon, N. M., & Ismail-Fitry, M. R. (2021). Effects of mixing duration and raw materials on the physicochemical, microstructural and sensorial properties of sausages prepared from red tilapia (Oreochromis sp.). Asian Fisheries Science, 34, 355-364.

Perchonok, M. H., & Regenstein, J. M. (1986). Stability at comminution chopping temperatures of model chicken breast muscle emulsions. Meat Science, 16(1), 17-29.

Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry, 193, 265-275.

Ramle, N. A., Zulkunain, M., & Ismail-Fitry, M. R. (2021). Replacing animal fat with edible mushrooms: A strategy to produce high-quality and low-fat buffalo meatballs. International Food Research Journal, 28(5), 905-915.

Ismail, M. A., Chong, G. H., & Ismail-Fitry, M. R. (2021). Comparison of the microstructural, physicochemical and sensorial properties of buffalo meat patties produced using bowl cutter, universal mixer and meat mixer. Journal of Food Science and Technology, 58(12), 4703-4710.

Hamm, R. (1961). Biochemistry of meat hydration. Advances in Food Research, 10, 355-463.

Acton, J. C., Ziegler, G. R., Burge Jr, D. L., & Froning, G. W. (1983). Functionality of muscle constituents in the processing of comminuted meat products. CRC Critical Reviews in Food Science and Nutrition, 18(2), 99-121.

Mandigo, R. W. & Sullivan, G. A. (2014). Chemistry and physics of comminuted products - Emulsion and batter. In M. Dikeman, & C. Devine (Eds.), Encyclopedia of Meat Science Vol. 1 (pp. 283-288). Academic Pres.

Sanchez, P. D. C., Arogancia, H. B. T., Boyles, K. M., Pontillo, A. J. B., & Ali, M. M. (2022). Emerging nondestructive techniques for the quality and safety evaluation of pork and beef: Recent advances, challenges, and future perspectives. Applied Food Research, 2(2), 100147.

Van Laack, R. L. J. M.. (1999). The role of proteins in water-holding capacity of meat. In Y. L. Xiong, C.-T. Ho, & F. Shahidi (Eds.), Quality attributes of muscle foods (pp.309-318). Springer.

Egbert, W. R. & Payne, C. T. (2009). Plant Proteins. In R. Tarté (Ed.), Ingredients in Meat Products: Properties, Functionality and Applications (111–130). Springer Science & Business Media.

Hunt, M. C. & King, D. A. (2012). AMSA Meat Color Measurement Guidelines. http://www.meatscience.org.

Aslinah, L. N. F., Mat Yusoff, M., & Ismail-Fitry, M. R. (2018). Simultaneous use of adzuki beans (Vigna angularis) flour as meat extender and fat replacer in reduced-fat beef meatballs (bebola daging). Journal of Food Science and Technology, 55, 3241-3248.

Levine, H. & Finley, J. W. (1999). Texture. In J. M. DeMan, J. W. Finley, W. J. Hurst, & C. Y. Lee (Eds.), Principles of Food Chemistry (pp. 329-364). Aspen Publishers.

Appell, M., Hurst, W. J., Finley, J. W. & DeMan, J. M. (1999). Amino acids and proteins. In J. M. deMan, J. W. Finley, W. J. Hurst, & C. Y. Lee (Eds.), Principles of Food Chemistry (pp. 117-164). Aspen Publishers.

Ishiwatari, N., Fukuoka, M., & Sakai, N. (2013). Effect of protein denaturation degree on texture and water state of cooked meat. Journal of Food Engineering, 117(3), 361-369.