Biosynthesis and Characterization of Silver Nanoparticles using Single Garlic Callus Extract (Allium sativum L.)

Yanti Puspita Sari; Amanda Qory Suchi; Rudy Agung Nugroho

doi:10.11113/mjfas.v19n4.2944

Authors

Yanti Puspita Sari Department of Biology, Faculty of Mathematics and Natural Sciences, Mulawarman University, Jl. Barong Tongkok No. 4 Gn. Kelua, Samarinda, East Kalimantan 75123, Indonesia https://orcid.org/0000-0003-4882-8440
Amanda Qory Suchi Department of Biology, Faculty of Mathematics and Natural Sciences, Mulawarman University, Jl. Barong Tongkok No. 4 Gn. Kelua, Samarinda, East Kalimantan 75123, Indonesia
Rudy Agung Nugroho Animal Physiology, Development, and Molecular Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Mulawarman University, Jl. Barong Tongkok, Gunung Kelua Campus, Samarinda, East Kalimantan 75123, Indonesia https://orcid.org/0000-0001-9006-7329

DOI:

https://doi.org/10.11113/mjfas.v19n4.2944

Keywords:

Silver nanoparticle, biosynthesis, stability, garlic, callus

Abstract

Nanotechnology is a relatively new and innovative field with huge potential for application in the food and drug industries. Due to their excellent physicochemical and biological properties, silver nanoparticles (AgNPs) are often utilized in various applications and have been the subject of substantial research. AgNP synthesis using plant extracts has recently gained popularity due to their environmental friendliness, affordability, and potent functionality in several applications. The present study evaluated the biosynthesis, stability, and characteristics of AgNPs using a single garlic callus extract (Allium sativum L.) AgNPs were synthesized using single garlic callus extract (AgNPs-As) by adding 1 mM AgNO₃ to ethanolic extracts of single garlic callus at a 1:9 ratio, incubating at 35 °C for 48 h, and observing the colloidal color change. Spectrophotometry (absorption at 200–800 nm), SEM, EDX, PSA, FTIR, and XRD were used for their characterization. The present study showed a colloidal color change to brown, indicating the formation of AgNPs-As. The characterization of AgNPs-As using SEM, EDX, PSA, and XRD revealed a spherical morphology with an average size of 201.9 nm. Several active compounds were identified from different peaks, indicating the presence of several types of biological functional groups, such as alkaloids, terpenoids, carbonyl groups, esters, halides, and alcohol were also confirmed using FTIR spectroscopy.

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