The First Next-Generation Sequencing Metabarcoding Dataset on Faecal Bacterial Diversity from the Southern River Terrapin, Batagur affinis ssp.

Mohd Hairul Mohd Salleh; Yuzine Esa

doi:10.11113/mjfas.v19n1.2802

Authors

Mohd Hairul Mohd Salleh ᵃDepartment of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; ᵇRoyal Malaysian Customs Department, Persiaran Perdana, Presint 2, 62596 Putrajaya, Malaysia
Yuzine Esa ᵃDepartment of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; ᶜInternational Institute of Aquaculture and Aquatic Sciences, Universiti Putra Malaysia, Lot 960 Jalan Kemang 6, 71050 Port Dickson, Negeri Sembilan, Malaysia

DOI:

https://doi.org/10.11113/mjfas.v19n1.2802

Keywords:

NGS metabarcoding dataset, Tuntung, bacteria, 16S rRNA, V3-V4 gene region

Abstract

The Southern River terrapin, Batagur affinis ssp., has the first data on faecal bacterial diversity from next-generation sequencing (NGS). This dataset describes the bacterial diversity of the Southern River terrapin, locally known as Tuntung. Batagur affinis spp. are freshwater turtles listed as critically endangered on the International Union for Conservation of Nature (IUCN) Red List since 2000. This is the first dataset on the faecal bacterial diversity of Batagur affinis ssp., and the data provided here can be used to comprehensively understand the microbiome's community composition. Seven faeces samples were collected aseptically from captive (N = 5) and wild (N = 2) adult B. affinis ssp. while crossing Peninsular Malaysia's east and west coasts. The data was acquired by metabarcoding using 16S rRNA. The amplicons were further analysed using the SILVA and DADA2 pipelines. The V3-V4 of the 16S rRNA gene region was amplified, and the amplicons were sequenced on the Illumina MiSeq system. In total, 297 bacterial communities' taxonomic profiles (phylum to genus) have been determined. The data for this metagenome can be found in the BioSample Submission Portal as Bio-Project PRJNA767629 and Sequence Read Archive (SRA) accession numbers from SAMN21919713 to SAMN21919722.

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