Antibacterial activity of CTAB-modified zeolite NaY with different CTAB loading


  • Mashitah Mad Salim
  • Nik Ahmad Nizam Nik Malek
  • Nur Isti’anah Ramli
  • Siti Aishah Mohd Hanim
  • Salehhuddin Hamdan



Cetyltrimethyl ammonium bromide (CTAB), Zeolite NaY, Cation Exchange Capacity, Escherichia coli, Staphylococcus aureus


The antibacterial activity of cetyltrimethyl ammonium bromide (CTAB) –Modified Zeolite NaY against Escherichia coli and Staphylococcus aureus was examined in agar disk diffusion (Kirby-Bauer) method. The diameter of the inhibition zones for E. coli and S. aureus, increased from 1.5 to 1.7 cm and 1.9 to 2.0 cm, respectively, with the increasing concentrations of CTAB adsorbed on Zeolite NaY from percent coverage of the External Cation Exchange Capacity of Zeolite NaY: 0.5 to 5.0. CTAB-Modified Zeolite NaY showed effective antimicrobial activity against S. aureus compared to E. coli.




J.-B.D. Green, T. Fulghum, M.A. Nordhous, Science against microbial pathogens: communicating current research and technological advances, In: Mendez-Vilas, A. (Ed.), Microbiology Book Series-Number 3, vol. 1, Formatex Research Center, 2011, p. 84-98

J. Hrenovic, J. Milenkovic, T. Ivankovic, N. Rajic, J. Hazard. Mater., 201-202 (2012) 260.

D. W. Breck, Zeolite Molecular Sieves: structure, chemistry, and use, John Wiley & Sons, Canada, 1974, p. 536

K. Hegstad, S. Langsrud, B. T. Lunestad, A. A. Scheie, M. Sunde, S. P. Yazdankhah, Microb. Drug Resist.16 (2010) 91.

D. L. Dyer, K. B. Gerenraich, US Patent 5994383 (1999)

A.J. Isquith, E.A. Abbott, P.A. Waters, Appl. Microbiol., 24 (1972) 859.

W.B. Hugo, J. Appl. Bacteriol., 30 (1967) 27.

A.N. Petrocci, Surface-active reagents: quaternary ammonium compounds. in: S.S. Block (Ed.) Disinfection sterilization and preservation, Lea and Febiger, Philadelphia, PA, 1983, p. 309

I.-F. Tsao, H.Y. Wang, C.Jr. Shipman, Biotechnol. Bioeng. 34 (1989) 639.

D. Schulze-Makuch, R.S. Bowman, S.D. Pillai, H. Guan, Ground Water Monit. R., 23(4) (2003) 68.

M. Abbaszadegan, B. Mayer, N. Ryu, N.H. Nwachuku, J. Environ. Sci. Heal., 41 (2006) 1201.

G. Ozdemir, S. Yapar, M.H. Limoncu, Appl. Clay Sci., 72 (2013) 201.

T. Moretro, G.S. Hoiby-Pettersen, C.K. Halvorsen, S. Langsrud, Food Control, 28 (2012) 118.

H.-H. Cheng, C.-C. Hsieh, C.-H. Tsai, Aerosol Air Qual. Res., 12 (2012) 409.

Z. Hagiwara, S. Hoshino, H. Ishino, S. Nohara, K. Tagawa, K. Yamanaka, US Patent 4911899 (1990)

A.M. Yusof, N.A.N.N. Malek, J. Hazard. Mater. 162 (2009) 1019.

G.V. Scott, Anal. Chem., 40 (1968) 768.

National Committee for Clinical Laboratory Standards, Performance standards of antimicrobial disk susceptibility test, Approved Standard M02-A8, Wayne, PA: NCCLS, 2000

H.-P. Chao, S.-H. Chen, Chem. Eng. J., 193-194 (2012) 283.

M. Ghiaci, R. Kia, A. Abbaspur, F. Seyedeyn-Azad, Sep. Purif. Technol., 40 (2004) 285.

M. Ghiaci, A. Abbaspur, R. Kia, F. Seyedeyn-Azad, Sep. Purif. Technol., 40 (2004) 217.

N.A.N.N. Malek, N.S. Malek, in 2nd International Conference on Chemistry and Chemical Process (ICCCP), APCBEE Procedia 3, 5-6 May 2012, Kuala Lumpur, Malaysia, 2012, p. 134-139

Z. Li, L. Gallus, Appl. Clay Sci., 35 (2007) 250.

O. Yousheng, X. Yushan, T. Shaozao, S. Qingshan, C. Yiben, J. Rare Earth, 27 (2009) 858.

P. Herrera, R.C. Burghardt, T.D. Phillips, Vet. Microbiol. 74(1999) 259.