Extracellular matrix (ECM) and cell adhesion molecules (CAMs) gene expression in brain GD-10, hippocampal cell (mHT-22 Cell Line), human glioblastoma-astrocytoma (hLN-405), and rat glioma cell (rF98) using real time PCR.
DOI:
https://doi.org/10.11113/mjfas.v10n2.260Keywords:
vimentin, brain, Hippocampal Cell, glioblastoma-astrocytoma, glioma cell, brain E-10Abstract
The aim of this study is to compare ECM and CAMs gene expression in hippocampal cell, glioblastoma-astrocytoma and glioma cell using real time PCR at gestation day of 10 (GD-10)
Result of real time PCR showed that expression level of extracelluler matrix of vimentin was higher than the expression of fibronectin, Neural cell Adhesion molecule (Ncam), neurofilament high (Nfh), neurofilament medium (NFm), neurofilament low (Nfl) and tenascin. The expression of vimentin in the brain also shows the highest when compare to the expression in the human glioblastoma-astrocytoma (hLN-405), rat glioma cell (rF98) and mouse hippocampal cell line (mHT-22). The number of vimentin gene copy was 538.554, whereas in the culture of hLN-405 was 2.246.309, in culture of rF98 was 974.368 and mHT-22 was 1.542.529. These findings suggested that vimentin most dominant expressed compare to the another gen. Expression of vimentin was gradually lower from astrocyte cell (hLN-405 or human glioblastoma-astrocytoma), then in hippocampus cell, glioma cell and embryonic brain cell.
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GRAPHICAL ABSTRACT
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