Effect of soil extracts concentration on specific growth rate and lipid content of chlorella vulgaris in bolds basal medium
DOI:
https://doi.org/10.11113/mjfas.v10n2.259Keywords:
Specific growth rate, Chlorella vulgaris, Soil extract, Bolds basal mediumAbstract
Effective nutrient medium is the key factor that significantly influences the specific growth rate and the final concentration of microalgae. The main objective of this study was to optimize the microalgal growth in mass culture system under the effect of soil extract in modified Bolds Basal medium. Chlorella vulgaris was grown aseptically for 10 days at five different concentrations of soil extract (10, 20, 30, 40, 50ml/L) at 25°C cultivation temperature. After 10 days of cultivation, Chlorella vulgaris showed maximum cell concentration of 5x107/ml at 30ml/L of soil extract concentration which corresponds to the maximum specific growth rate 1.56μd-1 with the doubling rate of 2.25d-1 andlipid yield of 22.74 % of dry weight.
________________________________________
GRAPHICAL ABSTRACT
References
G. Araujo, L.J. Matos, L.R Gonçalves, F.R. Fernandez, W.R. Farias. Bioresour. Technol. 102 (2011) 5248.
B. Wang, Q. Li, Y.N. Wu, C.Q. Lan Appl. Microbiol. Biotechnol. 79 (2008) 707.
J. Gressel. Plant Sci. 174 (2008) 246.
Y. Chisti. Biotechnol. Adv. 25 (2007) 294.
J.C. Ogbonna, H.Tanaka . J. Appl. Phycol. 12 (2000) 207.
J.U. Grobbelaar. Biotechnol and applied Phycology. Blackwell (2004) 97.
C. U. Ugwu, C.H. Aoyagi, H. Uchiyama. Photosynthetica 45 (2007) 309.
K.K Christensen. Water Air Soil Pollut. 99 (1997) 265.
EW. Becker. Cambridge, UK Cambridge University Press. (1994) 56.
N. Aagot, O. Nybroe, P. Nielson, K. Johsen, Appl. Environ. Microbiol. 67(2001) 5233.
G.S. William, H.A. Susan A. Waco, USA 43 (1998) 1467.
N. Subhasha, P.R. Monika, S, Rupali, Ame. J. Biochem Biotechnol. 7 (2011) 124.
M. Chen, H. Tang, H. Ma, T.C. Holland, K.Y. Simon, S.O. Sally. Bioresour. Technol. 102 (2011) 1649
E. Molina, F. Grima, G. A. Fernández, F. G. Camacho, Y.ChistI. J. Biotechnol. 70 (1999) 231.
S. Wahidin, A.Idris, S.R.M. Shaleh. Bioresour. Technol. 129 (2013) 7.
A. Norici, A. Dalsass, M Giordano. Physiologia Plantarum 116, (2002.) 186.
W.B. Kong, H. Yang, Y Cao. T,Song.H, Hua.S.F,Xia.C.G Food.Technol.Biotechnol.51 (2013) 62.
A.Ilavarasi, D. Mubarakali, R. Praveen kumar, E.Baldev, N Thajuddin. Biotechnology. 10 (2011) 540.
