Enantioseparation of enilconazole using cyclodextrin-modified MEKC


  • Dadan Hermawan
  • Ahmad Nor Shafiq Ahmad Kamali
  • Wan Aini Wan Ibrahim
  • Mohd Marsin Sanagi




Enantioseparationn, Enilconazole, CCD-MEKC, HP-γγ- CD,


Enantiomeric separation of enilconazole has been successfully achieved using cyclodextrin modified micellar electrokinetic chromatography (CD-MEKC) method with HP-γ- CD as chiral selector. Two parameters which were HP-γ-CD concentration and SDS concentration have been optimized by investigating their effects on resolution and analysis time. Enantioresolution of enilconazole was achieved by the optimized CD-MEKC system containing 40 mM HP-γ- CD, 40 mM SDS and 20 mM phosphate buffer (pH 8) with resolution (Rs = 1.3) and analysis time within 9 min. Good repeatabilities were obtained in term of RSD% (n = 3) for migration time, peak area and peak height ranging from 0.07 to 6.1 %. The optimized CD-MEKC method was a simple, less solvent used, and able to perform enantiomeric separation of enilconazole with a good resolution and relatively short analysis time.


J. Mc Conathy,M. J. Owens, J. Clin Psychiatry. 5, 2003, 70-73.

D. Ortelli, P. Edder, P., C. Corvi, Food Addit. Contam. 2005, 423–428.

B.L. Chu, B.Y. Guo, Z. Peng, Z. Wang, G. Guo, J.M. Lin, J. Sep. Sci. 30, 2007, 923 – 929.

N. Yoshioka, Y. Akiyama, K. Teranishi, J. Chromatogr. A.1022, 2004, 145–150.

N. Yoshioka, Y. Akiyama, T. Matsuoka, T. Mitsuhashi, Food Control. 21, 2010, 212–216.

B. Chankvatadze, G. Endresz, G. Blaschke, J. Chromatogr. A. 700, 1995, 43-49.

E. Watanabe, Y. Yoshimura, Y. Yuasa, H. Nakazawa, Anal.Chim. Acta. 433, 2001, 199–206.

FDA policy, Statement for the Development of New Stereoisomers Drugs, (1992), FDA, Rockville, MD.

S. Fanali, M. Cristalli, R. Vespalec, P. Bocek, Chiral separation in capillary electrophoresis, (1994), New York: VCH.