Physio-biochemical Responses of In Vitro Cooking Banana Musa paradisiaca cv Lang towards Pseudo Induced Drought Stress by Polyethylene Glycol (PEG)
DOI:
https://doi.org/10.11113/mjfas.v17n6.2341Keywords:
Antioxidant enzyme activity, chlorophyll content, drought stress, Musa paradisiaca cv Lang, polyethylene glycol,Abstract
Musa paradisiaca cv Lang belongs to cooking banana group, and it has high potential to be used in banana chips production. Like other cultivars, M. paradisiaca cv Lang is susceptible towards water shortage, therefore affecting banana growth and productivity. In this study, to mimic the drought condition, pseudo-drought stress was given to in vitro Lang banana seedlings by adding polyethylene glycol (PEG). Overall, decrement of roots length and chlorophyll (Chl) content was displayed by the seedlings exposed to 1%, 2%, 3%, 4%, and 5% (w/v) of PEG after three weeks of exposure. The proline content, total soluble protein content, and antioxidant capacity in leaf and roots, however, countered differently towards different levels of drought. Proline content showed the highest in leaf of 2% (w/v) PEG-treated seedling (12.66±0.38 µmoles/g) while the total soluble protein content showed the highest in roots of 5% (w/v) of PEG-treated seedling (30.65±1.07 mg/g FW). Antioxidant capacity of stressed seedlings revealed the catalase (CAT), guaiacol peroxidase (POD), and ascorbate peroxidase (APX) activities were the highest in the leaf of 1% (w/v) (10.69±5.06 µmol/min/mg), 4% (w/v), (0.079±0.03 µmol/min/mg), and 5% (w/v) (9.11±8.47 µmol/min/mg) of PEG- treated seedlings, respectively. Meanwhile, the highest CAT, POD, and APX activities in the roots were determined in 3% (w/v) (0.49±0.04 µmol/min/mg), 2% (w/v) (0.03±0.02 µmol/min/mg), and 3% (w/v) (16.69±0.5 µmol/min/mg) of PEG-treated seedlings, respectively. These data show that PEG can be a priming agent to induce defense system at seedling stage of banana, which could enhance their survivability during ex vitro acclimatization.
References
Huang YF, Ang JT, Tiong YJ, Mirzaei M, Amin MZM (2016). Drought forecasting using SPI and EDI under RCP-8.5 climate change scenarios for Langat River Basin, Malaysia. Procedia Engineering 154:710-717.
Zandalinas SI, Mittler, R, Balfagón D, Arbona V, Gómez-Cadenas A (2018). Plant adaptations to the combination of drought and high temperatures. Physiologia Plantarum 162(1):2-12.
Ravi I, Uma S, Vaganan MM, Mustaffa MM (2013). Phenotyping bananas for drought resistance. Frontiers in Physiology 4:1-15.
Chutia J, Borah SP (2012). Water stress effects on leaf growth and chlorophyll content but not the grain yield in traditional rice (Oryza sativa Linn.) Genotypes of Assam, India II. protein and proline status in seedlings under peg induced water stress. American Journal of Plant Sciences 3(7):971-980.
Nansamba M, Sibiya J, Tumuhimbise R, Karamura D, Kubiriba J, Karamura E (2020). Breeding banana (Musa spp.) for drought tolerance: A review. Plant Breeding 139:685-696.
National Research Centre for Banana (ICAR) (2008). 2008 annual report. Trichy, India: Anon.
Abdullah FC, Vun YL, Wali HSM, Sundaraj Y, Khamis S (2011). Peeling the scientific facts of banana. Shah Alam, Selangor: International Islamic Academy for Life Science and Biotechnology.
Ramlan NN, Azzeme AM, Padzil KN, Mahmood M (2017). Influence of different extraction solvents on phytochemical content and antioxidant capacity extracted from pulp and flower of dessert and cooking bananas. Malaysian Journal of Biochemistry & Molecular Biology 2&3:10-16.
Said EM, Mahmoud RA, Al-Akshar R, Shafwat G (2015). Drought stress tolerance and enhancement of banana plantlets in vitro. Austin Journal of Biotechnology and Bioengineering 2(2):1040-1047.
Hamayun M, Khan SA, Shinwari ZK, Khan AL, Ahmad N, Lee IJ (2010). Effect of polyethylene glycol induced drought stress on physio-hormonal attributes of soybean. Pakistan Journal of Botany 42(2):977-986.
Saglam A, Terzi R, Demiralay M (2014). Effect of polyethylene glycol induced drought stress on photosynthesis in two chickpea genotypes with different drought tolerance. Acta Biologica Hungarica 65(2):178-188.
Azzeme AM, Abdullah SNA, Aziz MA, Wahab PEM (2017). Oil palm drought inducible DREB1 induced expression of DRE/CRT- and non-DRE/CRT-containing genes in lowland transgenic tomato under cold and PEG treatments. Plant Physiology and Biochemistry 112:129-151
Meher, Shivakrishn P, Reddy KA, Rao DM (2018). Effect of PEG-6000 imposed drought stress on RNA content, relative water content (RWC), and chlorophyll content in peanut leaves and roots. Saudi Journal of Biological Sciences 25(2):285-289.
Cai K, Chen X, Han Z, Wu X, Zhang S, Li Q, Nazir MM, Zhang G, Zeng F (2020). Screening of worldwide barley collection for drought tolerance: The assessment of various physiological measures as the selection criteria. Frontiers in Plant Science 11:1159.
Lodish H, Berk A, Zipursky SL, Matsudaira P, Baltimore D, Darnell J (2000). Molecular cell biology, 4th edition. United State, New York: W. H. Freeman.
Azzeme AM, Abdullah SNA, Aziz MA, Wahab PEM (2016). Oil palm leaves and roots differ in physiological response, antioxidant enzyme activities and expression of stress-responsive genes upon exposure to drought stress. Acta Physiologiae Plantarum 38(2):52-64.
Hasanuzzaman M, Bhuyan MHM, Zulfiqar F, Raza A, Mohsin SM, Mahmud JA, Fujita M, Fotopoulos V (2020). Reactive oxygen species and antioxidant defense in plants under abiotic stress: revisiting the crucial role of a universal defense regulator. Antioxidants 9(8):681-733.
Sharma P, Jha AB, Dubey RS, Pessarakli M (2012). Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany 2012:1-26.
Król A, Amarowicz R, Weidner S (2014). Changes in the composition of phenolic compounds and antioxidant properties of grapevine roots and leaves (Vitis vinifera L.) under continuous of long-term drought stress. Acta Physiologiae Plantarum 36(6):1491-1499.
Man D, Bao YX, Han LB, Zhang X (2011). Drought tolerance associated with proline and hormone metabolism in two tall fescue cultivars. Horticultural Science 46(7):1027-1032.
Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco cultures. Physiologia Plantarum 15(5):473-497.
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research 50:151–158.
Harborne JB (1973). Phytochemical methods. Chapman and Hall, London.
Arora A, Choudhary D, Agarwal G, Singh VP (2008). Compositional variation in β-carotene content, carbohydrate and antioxidant enzymes in selected banana cultivars. International Journal of Food Science and Technology 43(11):1913-1921.
Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72(1-2):248-254.
Bates LS, Walden RP, Teare ID (1973). Rapid determination of free proline for water-stress studies. Plant and Soil 39(1):205-207. https://doi.org/10.1007/BF00018060
Osmolovskaya N, Shumilina J, Kim A, Didio A, Grishina T, Bilova T, Keltsieva OA, Zhukov V, Tikhonovich I, Tarakhovskaya E, Frolov A (2018). Methodology of drought stress research: Experimental setup and physiological characterization. International Journal of Molecular Sciences 19(12):4089.
Shekhawat UKS, Srinivas L, Ganapathi TR (2011). MusaDHN-1, a novel multiple stress-inducible SK3-Type dehydrin gene, contributes affirmatively to drought- and salt-stress tolerance in banana. Planta 234(5):915-932.
Muthusamy M, Uma S, Backiyarani S, Saraswathi MS, Chandrasekar A (2016). Transcriptomic changes of drought-tolerant and sensitive banana cultivars exposed to drought stress. Frontiers in Plant Science 7:1609.
Zorrilla-Fontanesi Y, Rouard M, Cenci A, Kissel E, Do H, Dubois E, Carpentier SC (2016). Differential root transcriptomics in a polyploid non-model crop: The importance of respiration during osmotic stress. Scientific Reports 6(1):25683.
van Wesemael J, Hueber Y, Kissel E, Campos N, Swennen R, Carpentier S (2018). Homeolog expression analysis in an allotriploid non-model crop via integration of transcriptomics and proteomics. Scientific Reports 8(1):1353.
Ismail MR, Yusoff MK, Mahmood M (2004). Growth, water relations, stomatal conductance and proline concentration in water stressed banana (Musa spp.) plants. Asian Journal of Plant Sciences 3:709-713.
Avramova V, Abd Elgawad H, Vasileva I, Petrova AS, Holek A, Mariën J, Asard H, Beemster GT (2017). High antioxidant activity facilitates maintenance of cell division in leaves of drought tolerant maize hybrids. Frontiers in Plant Science. 8:84.
Brunner I, Herzog C, Dawes MA, Arend M, Sperisen C (2015). How tree roots respond to drought. Frontiers in Plant Science 6:547.
Ali Q, Ali S, Iqbal N, Javed MT, Rizwan M, Khaliq R, Shahid S, Perveen R, Alamri SA, Alyemeni MN, Wijaya L (2019). Alpha-tocopherol fertigation confers growth physio-biochemical and qualitative yield enhancement in field grown water deficit wheat (Triticum aestivum L.). Scientific Reports 9(1):12924.
Sadak MS, Abdall AM, Abd Elhamid EM, Ezzo MI (2020). Role of melatonin in improving growth, yield quantity and quality of Moringa oleifera L. plant under drought stress. Bulletin of the National Research Centre 44(1):1-13.
Mihailović N, Lazarević M, Dželetović Z, Vučković M, Đurđević M (1997). Chlorophyllase activity in wheat, Triticum aestivum L. leaves during drought and its dependence on the nitrogen ion form applied. Plant Science 129(2):141-146.
Gu S, Dai X, Xu Z, Niu Q, Jiang J, Liu, Y (2021). Molecular, structural and biochemical characterization of a novel recombinant chlorophyllase from cyanobacterium Oscillatoria acuminata PCC 6304. Microbial Cell Factories 20(1):1-12.
Haider MS, Kurjogi MM, Khalil-urRehman M, Pervez T, Songtao T, Fiaz M, Jogaiah S, Wang C, Fang J (2018). Drought stress revealed physiological, biochemical and gene-expressional variations in ‘Yoshihime’ peach (Prunus Persica L) cultivar. Journal of Plant Interactions 13(1):83-90.
Bidabadi SS, Mahmood M, Baninasab B, Ghobadi C (2012). Influence of salicylic acid on morphological and physiological responses of banana (Musa acuminata cv. ‘Berangan’, AAA) shoot tips to in vitro water stress induced by polyethylene glycol. Plant Omics Journal 5(1):33-39.
Surendar KK, Devi DD, Ravi I, Jeyakumar P, Velayudham K (2013). Water stress affects plant relative water content, soluble protein, total chlorophyll content and yield of Ratoon Banana. International Journal of Horticulture 3(17):96-103.
Guo YY, Yu HY, Kong DS, Yan F, Zhang YJ (2016). Effects of drought stress on growth and chlorophyll fluorescence of Lycium ruthenicum Murr. seedlings. Photosynthetica 54(4):524–531.
Mafakheri A, Siosemardeh A, Bahramnejad B, Struik PC, Sohrabi Y (2010). Effect of drought stress on yield, proline and chlorophyll content in three chickpea cultivars. Australian Journal of Crop Science 4(8):580-585.
Meena M, Divyanshu K, Kumar S, Swapnil P, Zehra A, Shukla V, Yadav M, Upadhyay RS (2019). Regulation of L-proline biosynthesis, signal transduction, transport, accumulation and its vital role in plants during variable environmental conditions. Heliyon 5(12):e02952.
Hayat S, Hayat Q, Alyemeni MN, Wani AS, Pichtel J, Ahmad A (2012). Role of proline under changing environments: a review. Plant Signaling and Behavior 7(11):1456-1466.
Rejeb BK, Abdelly C, Savouré A (2014). How reactive oxygen species and proline face stress together. Plant Physiology and Biochemistry 80:278-284.
Surendar KK, Devi DD, Ravi I, Jeyakumar P, Velayudham K (2013). Physiological and biochemical behavior in banana cultivars and hybrids under water deficit. African Journal of Agricultural Research 8(31): 4198-4208.
Agrawal L, Gupta S, Mishra SK, Pandey G, Kumar S, Chauhan PS, Chakrabarty D, Nautiyal CS (2016). Elucidation of complex nature of PEG induced drought-stress response in rice root using comparative proteomics approach. Frontiers in Plant Science 7:1466.
Amnan MA, Pua TL, Lau SE, Tan BC, Yamaguchi H, Hitachi K, Tsuchida K, Komatsu S (2021). Osmotic stress in banana is relieved by exogenous nitric oxide. PeerJ. 9:e10879.
Kapoor D, Bhardwaj S, Landi M, Sharma A, Ramakrishnan M, Sharma A (2020). The Impact of drought in plant metabolism: How to exploit tolerance mechanisms to increase crop production. Applied Sciences 10(16):5692.
Ti-da GE, Fang-gong S, Li-ping L, Yin-yan L, Guang-sheng Z (2006). Effects of water stress on the protective enzyme activities and lipid peroxidation in roots and leaves of summer maize. Agricultural Sciences in China 5(4):291-298.
Kumar RR, Karajol K, Naik GR (2011). Plant physiology effect of polyethylene glycol induced water stress on effect of polyethylene glycol induced water stress on physiological and biochemical responses in Pigeonpea (Cajanus cajan L. Millsp). Recent research in Science and Technology 3(1):148-152.
Guo YY, Yu HY, Yang MM, Kong DS, Zhang YJ (2018). Effect of drought stress on lipid peroxidation, osmotic adjustment and antioxidant enzyme activity of leaves and roots of Lycium ruthenicum Murr. seedling. Russian Journal of Plant Physiology 65:244–250.
Gou W, Zheng P, Tian L, Gao M, Zhang L, Akram NA, Ashraf M (2017). Exogenous application of urea and a urease inhibitor improves drought stress tolerance in maize (Zea mays L.). Journal of Plant Research 130(3):599-609.
Iqbal N, Hussain S, Raza MA, Yang CQ, Safdar ME, Brestic M, Aziz A, Hayyat MS, Asghar MA, Wang XC, Zhang J, Yang W, Liu J (2019). Drought tolerance of soybean (Glycine max L. Merr.) by improved photosynthetic characteristics and an efficient antioxidant enzyme activity under a split-root system. Frontiers in Physiology, 10:786.
Anjum NA, Sharma P, Gill SS, Hasanuzzaman M, Khan EA, Kachhap K, Mohamed AA, Thangavel P, Devi GD, Vasudhevan P, Sofo A, Khan NA, Misra AN, Lukatkin AS, Singh HP, Pereira E, Tuteja N (2016). Catalase and ascorbate peroxidase-representative H2O2 detoxifying heme enzymes in plants. Environmental Science and Pollution Research 23(19):19002-19029.
de Campos, MKF, de Carvalho K, de Souza FS, Marur CJ, Pereira LFP, Filho JCB, Vieira LGE (2011). Drought tolerance and antioxidant enzymatic activity in transgenic “Swingle” citrumelo plants over-accumulating proline. Environmental and Experimental Botany 72(2):242-250.
Caverzan A., Passaia G, Rosa SB, Ribeiro CW, Lazzarotto F, Margis-Pinheiro M (2012). Plant responses to stresses: role of ascorbate peroxidase in the antioxidant protection. Genetics and Molecular Biology 35:1011–1019.
Kitajima SK, Nii H, Kitamura M (2014). Recombinant Stromal APX defective in the unique loop region showed improved tolerance to hydrogen peroxide. Bioscience, Biotechnology, and Biochemistry 74(7):1501-1503.
