Preliminary Study on the Effect of Nitrogen and Potassium Fertilization on Phytochemical Content Quality of Gynura procumbens
DOI:
https://doi.org/10.11113/mjfas.v17n4.2053Keywords:
Agriculture, Gynura, Nitrogen, Phytochemical, PotassiumAbstract
Gynura procumbens is an herbaceous plant. Despite the progressive reports on the pharmacological properties, many are overlooking at the importance of agronomic requirements, such as fertilization, to produce high phytochemical content which have not been conclusively concluded. The study was carried out to examine the effects of N and K interaction on physiological and phytochemical quality; to identify compositions of phytochemicals, and to determine marker compounds. Physiological and phytochemical attributes were recorded in three harvests of triplicate samples to exhibit the trend for plant quality, and statistically analyzed. Generally, N and K interaction have affected phytochemical content significantly (p<0.05) with stronger effect on physiological and biochemical attributes (p<0.01). The results have demonstrated that the following combination of fertilizer, 0 kg/ha N and 30 kg/ha K; and 90 kg/ha N and 0 kg/ha K are high and low, respectively affecting metabolite content in the plant. Lowest rate of N, moderate of K had produced significant phytochemical contents. Meanwhile, caffeic acid and kaempferol were demonstrated as marker compounds in this study. Thus, phytochemical content can be further established through the selection of appropriate N and K rates and proper abiotic stress interaction.References
Ali, Z., Ganie, S. H., Narula, A., Abdin, M. Z., & Srivastava, P. S. (2012). Organic and inorganic fertilizers influence biomass production and esculin content in Cichorium intybus L. Journal of Phytology, 4(5), 55–60.
Aminifard, M. H., Aroiee, H., Nemati, H., Azizi, M., & Jaafar, H. Z. E. (2012). Fulvic acid affects pepper antioxidant activity and fruit quality. African Journal of Biotechnology, 11(68), 13179–13185.
Anulika, N. P., Ignatius, E. O., Raymond, E. S., Osasere, O.-I., & Abiola, A. H. (2016). The Chemistry Of Natural Product: Plant Secondary Metabolites. International Journal of Technology Enhancements and Emerging Engineering Research, 4(8), 1.
Armengaud, P., Sulpice, R., Miller, A. J., Stitt, M., Amtmann, A., & Gibon, Y. (2009). Multilevel Analysis of Primary Metabolism Provides New Insights into the Role of Potassium Nutrition for Glycolysis and Nitrogen Assimilation in Arabidopsis Roots. Plant Physiology, 150(2), 772–785.
Armstrong, D. L. (1998). Better Crops with Plant Food. (D.L. Armstrong & K. P. Griffin, Eds.), Better Crops with Plant Food (3rd Ed.). Georgia: Potash & Phosphate Institute.
Ashokkumar, K., Selvaraj, K., & Saradha Devi, K. M. (2013). Reverse phase-high performance liquid chromatography-diode array detector (RP-HPLC-DAD) analysis of flavonoids profile from curry leaf (Murraya koenigii L.). Journal of Medicinal Plants Research, 7(47), 3393–3399.
Awang, Y., Shaharom, A. S., Mohamad, R. B., & Selamat, A. (2009). Chemical and Physical Characteristics of Cocopeat-based Media Mixtures and their Effects on the Growth and Development of Celosia cristata. American Journal of Agricultural and Biological Science, 4(1), 63–71.
Baby, S., Johnson, A. J., Zachariah, E. J., & Hussain, A. A. (2017). Nepenthes pitchers are CO2-enriched cavities, emit CO2 to attract preys. Scientific Reports, 7(1), 1–10.
Bellés, J. M., Garro, R., Pallás, V., Fayos, J., Rodrigo, I., & Conejero, V. (2006). Accumulation of gentisic acid as associated with systemic infections but not with the hypersensitive response in plant-pathogen interactions. Planta, 223, 500–511.
Caretto, S., Linsalata, V., Colella, G., Mita, G., & Lattanzio, V. (2015). Carbon Fluxes between Primary Metabolism and Phenolic Pathway in Plant Tissues Under Stress. International Journal of Molecular Sciences, 16, 26378–26394.
Chen, Y., Yu, M., Zhu, Z., Zhang, L., & Guo, Q. (2013). Optimisation of Potassium Chloride Nutrition for Proper Growth, Physiological Development and Bioactive Component Production in Prunella vulgaris L. PLoS ONE, 8(7), 1–7.
Chrysargyris, A., Drouza, C., & Tzortzakis, N. (2017). Optimization of potassium fertilization/nutrition for growth, physiological development, essential oil composition and antioxidant activity of Lavandula angustifolia Mill. Journal of Soil Science and Plant Nutrition, 17(2), 291–306.
Davies, M. J., Atkinson, C. J., Burns, C., Woolley, J. G., Hipps, N. A., Arroo, R. R. J., … Bentley, S. (2009). Enhancement of artemisinin concentration and yield in response to optimization of nitrogen and potassium supply to Artemisia annua. Annals of Botany, 104, 315–323.
Drew, S. W., & Demain, A. L. (1977). Effect of Primary Metabolites on Secondary Metabolism. Annual Review of Microbiology, 31, 343–356.
Farrell, R. E. (2007). The Regulation of Gene Expression in Plants and Animals. In C. L. Bassett (Ed.), Regulation of Gene Expression in Plants (pp. 1–38). New York: Springer.
Figueiredo, A. C., Barroso, J. G., Pedro, L. G., & Scheffer, J. J. C. (2008). Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour and Fragrance Journal, 23, 213–226.
Fonseca, J. M., Rushing, J. W., Rajapakse, N. C., Thomas, R. L., & Riley, M. B. (2006). Potential Implications of Medicinal Plant Production in Controlled Environments: The case of Feverfew (Tanacetum parthenium). HortScience, 41(3), 531–535.
Galieni, A., Di Mattia, C., De Gregorio, M., Speca, S., Mastrocola, D., Pisante, M., & Stagnari, F. (2015). Effects of nutrient deficiency and abiotic environmental stresses on yield, phenolic compounds and antiradical activity in lettuce (Lactuca sativa L.). Scientia Horticulturae, 187, 93–101.
Giedyk, M. (2011). HPLC as an analytical and preparative method. Warsaw: Polish Academy of Science.
Hansen, J., & Moller, I. B. (1975). Analysis of starch and soluble sugars with Anthrone reagent. Anal Biochemistry, 68, 87–94.
Hanudin, E., Wismarini, H., Hertiani, T., & Sunarminto, B. H. (2012). Effect of shading, nitrogen and magnesium fertilizer on phyllanthin and total flavonoid yield of Phyllanthus niruri in Indonesia soil. Journal of Medicinal Plants Research, 6(30), 4586–4592.
Hashiguchi, A., Tian, J., & Komatsu, S. (2017). Proteomic Contributions to Medicinal Plant Research: From Plant Metabolism to Pharmacological Action. Proteomes, 5(35), 1–12.
Hew, C. S., & Gam, L. H. (2010). The Identification of High Abundant Proteins in the Leaves of Gynura procumbens. Biotechnology and Biotechnological Equipment, 24(4), 2132–2136.
Heydarizadeh, P. (2016). Photosynthetic Organisms Under Stress. Universite’ du Maine.
Hunt, R. (2003). Growth Analysis, Individual Plants. In B. Thomas, D. J. Murphy, & D. Murray (Eds.), Encyclopaedia of Applied Plant Sciences (Ed., pp. 575–579). London: Academic Press.
Ibrahim, M. H., Ismail, A., Omar, H., Mohd Nadzir, M. N. H., & Mohd Zain, N. A. (2017). Primary, Secondary Metabolites, Biochemical and Antioxidant Activity of Orthosiphon staminues Benth (Misai Kucing) under Cadmium Exposure. Annual Research & Review in Biology, 19(1), 1–14.
Ibrahim, M. H., Ismail, A., Omar, H., & Zain, N. A. M. (2017). Application Effects of Cadmium and Humic Acid on the Growth, Chlorophyll Fluorescence, Leaf Gas Exchange and Secondary Metabolites in Misai Kucing (Orthosiphon stamineus) Benth. Annual Research & Review in Biology, 18(3), 1–14.
Ibrahim, M. H., & Jaafar, H. Z. E. (2012). Impact of Elevated Carbon Dioxide on Primary, Secondary Metabolites and Antioxidant Responses of Eleais guineensis Jacq. (Oil Palm) Seedlings. Molecules, 17, 5195–5211.
Ibrahim, M. H., Jaafar, H. Z. E., Karimi, E., & Ghasemzadeh, A. (2012). Primary, Secondary Metabolites, Photosynthetic Capacity and Antioxidant Activity of the Malaysian Herb Kacip Fatimah (Labisia Pumila Benth) Exposed to Potassium Fertilization under Greenhouse Conditions. International Journal of Molecular Sciences, 13, 15321–15342.
Ibrahim, M. H., Jaafar, H. Z. E., Karimi, E., & Ghasemzadeh, A. (2013). Impact of Organic and Inorganic Fertilizers Application on the Phytochemical and Antioxidant Activity of Kacip Fatimah (Labisia pumila Benth). Molecules, 18, 10973–10988.
Ibrahim, M. H., Jaafar, H. Z. E., Karimi, E., & Ghasemzadeh, A. (2014). Allocation of Secondary Metabolites, Photosynthetic Capacity, and Antioxidant Activity of Kacip Fatimah (Labisia pumila Benth) in Response to CO2 and Light Intensity. The Scientific World Journal, 2014, 1–13.
Ibrahim, M. H., Jaafar, H. Z. E., Rahmat, A., & Rahman, Z. A. (2011). Effects of Nitrogen Fertilization on Synthesis of Primary and Secondary Metabolites in Three Varieties of Kacip Fatimah (Labisia pumila blume). International Journal of Molecular Sciences, 12, 5238–5254.
Iskander, M. N., Song, Y., Coupar, I. M., & Jiratchariyakul, W. (2002). Antiinflammatory screening of the medicinal plant Gynura procumbens. Plant Foods for Human Nutrition, 57, 233–244.
Ismail, A. H., Idris, A. N., Amina, M. M., Ibrahim, A. S., & Audu, S. A. (2015). Phytochemical studies and thin layer chromatography of leaves and flower extracts of Senna siamea lam for possible biomedical applications. Journal of Pharmacognosy and Phytotherapy, 7(3), 18–26.
Jaafar, H. Z. E., Ibrahim, M. H., & Fakri, N. F. M. (2012). Impact of Soil Field Water Capacity on Secondary Metabolites, Phenylalanine Ammonia-lyase (PAL), Maliondialdehyde (MDA) and Photosynthetic Responses of Malaysian Kacip Fatimah (Labisia pumila Benth). Molecules, 17(6), 7305–7322.
John, E. C. (1995). Assays for Total Protein. In E. C. John, M. D. Ben, L. P. Hidde, W. S. David, & T. W. Paul (Eds.), Current Protocols in Protein Science (Ed., pp. 1–24). London: John Wiley & Sons.
Kaewseejan, N., & Siriamornpun, S. (2015). Bioactive components and properties of ethanolic extract and its fractions from Gynura procumbens leaves. Industrial Crops and Products, 74, 271–278.
Kaewseejan, N., Sutthikhum, V., & Siriamornpun, S. (2015). Potential of Gynura procumbens leaves as source of flavonoid-enriched fractions with enhanced antioxidant capacity. Journal of Functional Foods, 12, 120–128.
Kaiser, D. E., Rosen, C. J., & Lamb, J. A. (2016). Potassium for Crop Production.
Khalid, K. A. (2006). Influence of water stress on growth, essential oil, and chemical composition of herbs (Ocimum sp.). International Agrophysics, 20, 289–296.
Kleczewski, N. M., Herms, D. A., & Bonello, P. (2010). Effects of soil type, fertilization and drought on carbon allocation to root growth and partitioning between secondary metabolism and ectomycorrhizae of Betula papyrifera. Tree Physiology, 30, 807–817.
Krishnan, V., Ahmad, S., & Mahmood, M. (2015). Antioxidant Potential in Different Parts and Callus of Gynura procumbens and Different Parts of Gynura bicolor. BioMed Research International, 1–7.
Larbat, R., Olsen, K. M., Slimestad, R., Løvdal, T., Bénard, C., Verheul, M., … Lillo, C. (2012). Influence of repeated short-term nitrogen limitations on leaf phenolics metabolism in tomato. Phytochemistry, 77, 119–128.
Latifa, I. C., & Anggarwulan, E. (2009). Nitrogen content, nitrate reductase activity, and biomass of kimpul (Xanthosoma sagittifolium) on shade and nitrogen fertilizer variation. Nusantara Bioscience, 1(2), 65–71.
Le Bot, J., Bénard, C., Robin, C., Bourgaud, F., & Adamowicz, S. (2009). The “trade-off” between synthesis of primary and secondary compounds in young tomato leaves is altered by nitrate nutrition: Experimental evidence and model consistency. Journal of Experimental Botany, 60(15), 4301–4314.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein Measurement with the Folin Phenol Reagent. Journal of Biological Chemistry, 193(1), 265–275.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (2000). Total protein estimation by Lowry’s method. Experiment BL 301. Maine: Maine College of Art.
Macadam, J. W., Volenec, J. J., & Nelson, C. J. (1989). Effects of Nitrogen on Mesophyll Cell Division and Epidermal Cell Elongation in Tall Fescue Leaf Blades’. Plant Physiology, 89, 549–556.
Marchese, J. A., Ferreira, J. F. S., Rehder, V. L. G., & Rodrigues, O. (2010). Water deficit effect on the accumulation of biomass and artemisinin in annual wormwood (Artemisia annua L., Asteraceae). Brazilian Journal of Plant Physiology, 22(1), 1–9.
Mishra, S., Tyagi, A., Singh, I. ., & Sangwan, R. S. (2006). Changes in lipid profile during growth and senescence of Catharanthus roseus leaf. Brazilian Journal of Plant Physiology, 18(4), 447–454.
Mohamad Bukhori, M. F., Jaafar, H. Z. E., & Ghasemzadeh, A. (2015). Watering and nitrogen and potassium fertilization: The significance of abiotic control on Gynura procumbens (Lour.) Merr herbs in Malaysia for better growth and secondary metabolite enrichment. Asia Pacific Journal of Molecular Biology and Biotechnology, 23(232), 303–313.
Mohd Zain, N. A., & Ismail, M. R. (2016). Effects of potassium rates and types on growth, leaf gas exchange and biochemical changes in rice (Oryza sativa) planted under cyclic water stress. Agricultural Water Management, 164, 83–90.
Mohd Zain, N. A., Ismail, M. R., Puteh, A., Mahmood, M., & Islam, M. R. (2014). Drought Tolerance and Ion Accumulation of Rice Following Application of Additional Potassium Fertilizer. Communications in Soil Science and Plant Analysis, 45, 2502–2514.
Mongkhonsin, B., Nakbanpote, W., Hokura, A., Nuengchamnong, N., & Maneechai, S. (2016). Phenolic compounds responding to zinc and/or cadmium treatments in Gynura pseudochina (L.) DC. extracts and biomass. Plant Physiology and Biochemistry, 109, 549–560.
Morat, N. M. (2013). Total Phenolic and Flavonoid Content of Three Species of Gynura. Universiti Teknologi MARA.
Mori, K., Sugaya, S., & Gemma, H. (2005). Decreased anthocyanin biosynthesis in grape berries grown under elevated night temperature condition. Scientia Horticulturae, 105, 319–330.
Mráz, P., Tarbush, E., & Müller-Schärer, H. (2014). Drought tolerance and plasticity in the invasive knapweed Centaurea stoebe s.l. (Asteraceae): Effect of populations stronger than those of cytotype and range. Annals of Botany, 114(2), 1–11.
Ng, H. K., Poh, T. F., Lam, S. K., & Hoe, S. Z. (2013). Potassium channel openers and prostacyclin play a crucial role in mediating the vasorelaxant activity of Gynura procumbens. BMC Complementary and Alternative Medicine, 13(188), 1–11.
Olkowski, A. A., Amarowicz, R., Peiquiang, Y., Mckinnon, J. J., & Maenz, D. D. (2003). A Rapid HPLC Method for Determination of Major Phenolic Acids in Plant Material. Polish Journal Of Food And Nutrition Sciences, 12(1), 53–57.
Olszewska, M. (2007). Quantitative HPLC Analysis of Flavonoids and Chlorogenic Acid in the Leaves and Inflorescences of Prunus serotina EHRH. Acta Chromatographica, 19, 253–269.
Ora, O. P., & Anekwe, G. E. (2013). Lipid composition of medicinal plants: Portulaca oleracea and Ficus thonningi. African Journal of Food Science, 7(5), 76–79.
Ossipov, V., Salminen, J. P., Ossipova, S., Haukioja, E., & Pihlaja, K. (2003). Gallic acid and hydrolysable tannins are formed in birch leaves from an intermediate compound of the shikimate pathway. Biochemical Systematics and Ecology, 31(1), 3–16.
Ozdal, T., Capanoglu, E., & Altay, F. (2013). A review on protein-phenolic interactions and associated changes. Food Research International, 51, 954–970.
Pal, P. K., Kumar, R., Guleria, V., Mahajan, M., Prasad, R., Pathania, V., … Ahuja, P. S. (2015). Crop-ecology and nutritional variability influence growth and secondary metabolites of Stevia rebaudiana Bertoni. BMC Plant Biology, 15(67), 1–16.
Pallez, L. C., & Dole, J. M. (2001). Maintaining Vegetative Potted Purple Velvet Plants. HortTechnology (Vol. 11). Oklahoma.
Pant, B. D., Pant, P., Erban, A., Huhman, D., Kopka, J., & Scheible, W. R. (2015). Identification of primary and secondary metabolites with phosphorus status-dependent abundance in Arabidopsis, and of the transcription factor PHR1 as a major regulator of metabolic changes during phosphorus limitation. Plant, Cell & Environment, 38, 172–187.
Pavloviĉ, A., Singerová, L., Demko, V., & Hudák, J. (2009). Feeding enhances photosynthetic efficiency in the carnivorous pitcher plant Nepenthes talangensis. Annals of Botany, 104(2), 307–314.
Rahimi, A., Sayadi, F., Dashti, H., & Tajabadi, A. (2013). Effects of water and nitrogen supply on growth , water-use efficiency and mucilage yield of isabgol (Plantago ovata Forsk). Journal of Soil Science and Plant Nutrition, 13(2), 341–354.
Ramakrishna, A., & Ravishankar, G. A. (2011). Influence of abiotic stress signals on secondary metabolites in plants. Plant Signaling and Behavior, 6(11), 1720–1731.
Rivai, H., Nurdin, H., Suyani, H., & Bakhtiar, A. (2011). Karakterisasi Ekstrak Daun Dewa [Gynura pseudochina (L.) DC] Dengan Kromatografi Cair Kinerja Tinggi. Jurnal Farmasi Indonesia, 5(3), 134–141.
Rosidah, Yam, M. F., Sadikun, A., & Asmawi, M. Z. (2008). Antioxidant potential of Gynura procumbens. Pharmaceutical Biology, 46(9), 616–625.
Sasidharan, S., Chen, Y., Saravanan, D., Sundram, K. M., & Latha, L. Y. (2011). Extraction, Isolation and Characterization of Bioactive Compounds from Plants Extracts. African Journal of Traditional Complementary & Alternative Medicine, 8(1), 1–10.
Sasikala, A., Linga Rao, M., & Savithramma, N. (2013). Quantification of Primary and Secondary Metabolites From Leaves and Stem Bark of Cochlospermum Religiosum (L) Alston. International Research Journal of Pharmacy, 4(8), 228–231.
Selmar, D., & Kleinwächter, M. (2013). Stress Enhances the Synthesis of Secondary Plant Products: The Impact of Stress-Related Over-Reduction on the Accumulation of Natural Products. Plant and Cell Physiology, 54(6), 817–826.
Seow, L. J., Beh, H. K., Umar, M. I., Sadikun, A., & Asmawi, M. Z. (2014). Anti-inflammatory and antioxidant activities of the methanol extract of Gynura segetum leaf. International Immunopharmacology, 23, 186–191.
Shams, K. A., Abdel-Azim, N. S., Tawfik, W. A., Hassanein, H. D., Saleh, M. A., & Hammouda, F. M. (2015). Green extraction techniques: Effect of extraction method on lipid contents of three medicinal plants of Apiaceae. Journal of Chemical and Pharmaceutical Research, 7(4), 1080–1088.
Shirazi, O. S., Khan Khattak, M. M. A., Mohd Shukri, N. A., & Mohd Nur Nasyriq, A. (2014). Determination of total phenolic, flavonoid content and free radical scavenging activities of common herbs and spices. Journal of Pharmacognosy and Phytochemistry, 3(3), 104–108.
Shitan, N. (2016). Secondary metabolites in plants: Transport and self-tolerance mechanisms. Bioscience, Biotechnology and Biochemistry, 80(7), 1283–1293.
Shoeva, O. ., Mock, H. P., Kukoeva, T. V., Börner, A., & Khlestkina, E. K. (2016). Regulation of the Flavonoid Biosynthesis Pathway Genes in Purple and Black Grains of Hordeum vulgare. PLoS ONE, 11(10), 1–16.
Sim, C. C., Zaharah, A. R., Tan, M. S., & Goh, K. J. (2015). Rapid Determination of Leaf Chlorophyll Concentration, Photosynthetic Activity and NK Concentration of Elaies guineensis via Correlated SPAD-502 Chlorophyll Index. Asian Journal of Agricultural Research, 9(3), 132–138.
Tamayo, P. R., Weiss, O., & Sanchez-moreiras, A. M. (2001). Gas Exchange Techniques in Photosynthesis and Respiration Infrared Gas Analyser. In M. J. R. Roger (Ed.), Handbook of Plant Ecophysiology Techniques (Ed., pp. 113–139). Netherlands: Kluwer Academic Publishers.
Teoh, W. Y., Wahab, N. A., Richardson, J. S. ., & Sim, K. S. (2016). Evaluation of antioxidant properties, cytotoxicity and acute oral toxicity of Gynura procumbens (Compositae). Sains Malaysiana, 45(2), 229–235.
Thomas, H. (2013). Senescence, ageing and death of the whole plant. New Phytologist, 197(3), 696–711.
Torres, A. P., & Lopez, R. G. (2010). Commercial Greenhouse Production - Measuring Daily Light Integral in a Greenhouse. Purdue Extension. Purdue.
Treder, J. (2008). The Effects of Cocopeat and Fertilization on the Growth and Flowering of Oriental Lily “Star Gazer.” Journal of Fruit and Ornamental Plant Research, 16, 361–370.
Wang, M., Liu, H., Dong, C., Fu, Y., & Liu, H. (2016). Elevated CO2 enhances photosynthetic efficiency, ion uptake and antioxidant activity of Gynura bicolor DC. grown in a porous-tube nutrient delivery system under simulated microgravity. Plant Biology, 18, 391–399.
Wu, T., Zhou, X., Deng, Y., Jing, Q., Li, M., & Yuan, L. (2011). In vitro studies of Gynura divaricata (L.) DC extracts as inhibitors of key enzymes relevant for type 2 diabetes and hypertension. Journal of Ethnopharmacology, 136, 305–308.
Wuyts, N., Dhondt, S., & Inzé, D. (2015). Measurement of plant growth in view of an integrative analysis of regulatory networks. Current Opinion in Plant Biology, 25, 90–97.
Wuyts, N., Palauqui, J. C., Conejero, G., Verdeil, J. L., Granier, C., & Massonnet, C. (2010). High-contrast three-dimensional imaging of the Arabidopsis leaf enables the analysis of cell dimensions in the epidermis and mesophyll. Plant Methods, 6(17), 1–14.
Yi, X., Zuo, J., Tan, C., Xian, S., Luo, C., Chen, S., … Luo, Y. (2016). Kaempferol, a flavonoid compound from Gynura medica induced apoptosis and growth inhibition in MCF-7 breast cancer cell. African Journal of Traditional, Complementary and Alternative Medicines, 13(4), 210–215.
Yu, Y., Yang, R., Wan, C., Xu, B., Cao, S., & Liu, W. (2010). Optimization of Total Flavonoid Compound Extraction from Gynura medica Leaf Using Response Surface Methodology and Chemical Composition Analysis. International Journal of Molecular Sciences, 11, 4750–4763.
Zheng, Z. L. (2009). Carbon and nitrogen nutrient balance signaling in plants. Plant Signaling & Behavior, 4(7), 584–591.
Zulkifle, N. (2006). The study of Gynura procumbens (Lour.) Merr. Crude extract using high performance liquid chromatography (HPLC). Universiti Sains Malaysia.