Effects of soybean co-ingestion with carbohydrate on postprandial glycemic, insulinemic and reactive oxygen species in healthy men: A pilot study
DOI:
https://doi.org/10.11113/mjfas.v16n5.1918Keywords:
Soybean, oxidative stress, postprandial, reactive oxygen species, glucoseAbstract
Postprandial hyperglycemia induced by excessive intake of high carbohydrate (CHO) foods and beverages promotes oxidative stress which can cause many health risks such as cardio-metabolic diseases and diabetes. Protein when co-ingested with CHO beverages, has been shown to lower the postprandial glycaemic and insulinemic, which may help to attenuate postprandial reactive oxygen species (ROS) and oxidative stress. Soybean contains isoflavone which may provide potential benefits in regulating postprandial glucose and insulin levels as well as providing protection against ROS production. The aim of this study was to investigate the effects of soybean added CHO beverage on postprandial glycemic, insulinemic and reactive oxygen species responses in healthy men. Eight male [age 20.0 (1.2) years, body weight 59.2 (6.2) kg] consumed 500 ml of CHO added with soybean (SOY + CHO), CHO added with whey protein (WHEY + CHO) and CHO alone (Control) after an overnight fast, in a randomized counterbalanced order crossover design, separated by a one week period. Venous blood samples were collected after overnight fast (baseline) and at 30, 60, 90 and 120 min after consumption of the beverage. The mean area under the insulin curve was lower in SOY + CHO trial compared to CHO + WHEY trial. Similarly, SOY + CHO tended to have a lower postprandial ROS response than CHO + WHEY. However, no significant difference was observed between all beverages in all parameters. Soybean-based beverage may yield lower effect on postprandial ROS suggesting lower oxidative stress due to lower insulinemic responses, compared to whey protein when co-ingested with CHOReferences
Kearney J. Food consumption trends and drivers. Philos Trans R Soc Lond B Biol Sci, 2010, 365(1554), 2793-2807, doi: 10.1098/rstb.2010.0149
Ceriello A, Assaloni R, Da Ros R, Maier A, Piconi L, Quagliaro L, Esposito K, Giugliano D. Effect of atorvastatin and irbesartan, alone and in combination, on postprandial endothelial dysfunction, oxidative stress, and inflammation in type 2 diabetic patients. Circulation, 2005, 111(19), 2518-2524, doi: 10.1161/01.CIR.0000165070.46111.9F
Monnier L, Mas E, Ginet C, Michel F, Villon L, Cristol J-P, & Colette C. Activation of oxidative stress by acute glucose fluctuations compared with sustained chronic hyperglycemia in patients with type 2 diabetes. JAMA, 2006, 295(14), 1681-1687, doi: 10.1001/jama.295.14.1681
Brownlee M, & Hirsc IB. (2006). Glycemic variability: A hemoglobin a1c–independent risk factor for diabetic complications. JAMA, 295(14), 1707-1708, DOI: 10.1001/jama.295.14.1707
Mapanga RF, & Essop MF. Damaging effects of hyperglycemia on cardiovascular function: spotlight on glucose metabolic pathways. Am J Physiol Heart Circ Physiol, 2016, 310(2), H153-173, doi: 10.1152/ajpheart.00206.2015
Busik JV, Mohr S, & Grant MB. Hyperglycemia-induced reactive oxygen species toxicity to endothelial cells is dependent on paracrine mediators. Diabetes, 2008, 57(7), 1952-1965, doi: 10.2337/db07-1520
Shao B, & Bayraktutan U. Hyperglycaemia promotes human brain microvascular endothelial cell apoptosis via induction of protein kinase C-ssI and prooxidant enzyme NADPH oxidase. Redox Biol, 2014, 2, 694-701, doi: 10.1016/j.redox.2014.05.005
Su H, Ji L, Xing W, Zhang W, Zhou H, Qian X, Wang X, Gao F, Sun X, & Zhang H. Acute hyperglycaemia enhances oxidative stress and aggravates myocardial ischaemia/reperfusion injury: role of thioredoxin-interacting protein. J Cell Mol Med, 2013, 17(1), 181-191, doi: 10.1111/j.1582-4934.2012.01661.x
Lin N, Li X-Y, Zhang H-M, Yang Z, & Su Q. MicroRNA-199a-5p mediates high glucose induced ROS production and apoptosis in INS-1 pancreatic B cells by targeting SIRT1. Eur Rev Med Pharmacol Sci, 2017, 21:1091-1098.
Callahan LA, & Supinski GS. Hyperglycemia induced diaphragm weakness is mediated by oxidative stress. Critical Care, 2014, 18(3): R88, doi: 10.1186/cc13855
Ebaid H, Salem A, Sayed A, & Metwalli A. Whey protein enhances normal inflammatory responses during cutaneous wound healing in diabetic rats. Lipids in Health and Disease, 2011, 10, 235. doi:10.1186/1476-511X-10-235
Nilsson M, Holst JJ, & Björck ME. Metabolic effects of amino acid mixtures and whey protein in healthy subjects: studies using glucose-equivalent drinks. American Journal of Clinical Nutrition, 2007, 85, 996-1004, doi: 10.1093/ajcn/85.4.996
Manders RJ, Hansen D, Zorenc AH, Dendale P, Kloek J, Saris WH, & van Loon LJ. Protein co-ingestion strongly increases postprandial insulin secretion in type 2 diabetes patients. J Med Food, 2014, 17(7), 758-763, doi: 10.1089/jmf.2012.0294
Messina M. Soy and Health Update: Evaluation of the Clinical and Epidemiologic Literature. Nutrients, 2016, 8(12), doi: 10.3390/nu8120754
Yu J, Bi X, Yu B, & Chen D. Isoflavones: Anti-Inflammatory Benefit and Possible Caveats. Nutrients, 2016, 8(6), doi: 10.3390/nu8060361
Chang JH, Kim MS, Kim TW, & Lee SS. Effects of soybean supplementation on blood glucose, plasma lipid levels, and erythrocyte antioxidant enzyme activity in type 2 diabetes mellitus patients. Nutrition Research and Practice, 2008, 2(3), 152-157, doi: 10.4162/nrp.2008.2.3.152
Tatsumi Y, Morimoto A, Deura K, Mizuno S, Ohno Y, & Watanabe S. Effects of soybean product intake on fasting and postload hyperglycemia and type 2 diabetes in Japanese men with high body mass index: The Saku Study. J Diabetes Investig, 2013, 4(6), 626-633, doi: 10.1111/jdi.12100
Ceriello A. Evidence for an Independent and Cumulative Effect of Postprandial Hypertriglyceridemia and Hyperglycemia on Endothelial Dysfunction and Oxidative Stress Generation: Effects of Short- and Long-Term Simvastatin Treatment. Circulation, 2002, 106(10), 1211-1218, doi: 10.1161/01.cir.0000027569.76671.a8
Nivoit , Wiernsperger N, Moulin P, Lagarde M, & Renaudin C. Effect of glycated LDL on microvascular tone in mice: a comparative study with LDL modified in vitro or isolated from diabetic patients. Diabetologia, 2003, 46(11), 1550-1558. doi:10.1007/s00125-003-1225-2
Sima AV, Botez GM, Stancu CS, Manea A, Raicu M, & Simionescu M. Effect of irreversibly glycated LDL in human vascular smooth muscle cells: lipid loading, oxidative and inflammatory stress. J Cell Mol Med, 2010, 14(12), 2790-2802, doi: 10.1111/j.1582-4934.2009.00933.x
Spagnoli LG, Bonanno E, Sangiorgi G, & Mauriello A. Role of inflammation in atherosclerosis. J Nucl Med, 2007, 48(11), 1800-1815, doi: 10.2967/jnumed.107.038661
Akhavan T, Luhovyy BL, Brown PH, Cho CE, & Anderson GH. Effect of premeal consumption of whey protein and its hydrolysate on food intake and postmeal glycemia and insulin responses in young adults. Am J Clin Nutr, 2010, 91(4), 966-975, doi: 10.3945/ajcn.2009.28406
Akhavan T, Luhovyy BL, Panahi S, Kubant R, Brown PH, & Anderson GH. Mechanism of action of pre-meal consumption of whey protein on glycemic control in young adults. Journal of Nutritional Biochemistry, 2014, 25(1), 36-43, doi: 10.1016/j.jnutbio.2013.08.012
Allerton DM, Campbell MD, Gonzalez JT, Rumbold PL, West DJ, & Stevenson EJ. Co-Ingestion of Whey Protein with a Carbohydrate-Rich Breakfast Does Not Affect Glycemia, Insulinemia or Subjective Appetite Following a Subsequent Meal in Healthy Males. Nutrients, 2016, 8(3), 116, doi: 10.3390/nu8030116
Parker AM, & Watson RR. Chapter 16 - Lactose Intolerance. In R. R. Watson, R. J. Collier, & V. R. Preedy (Eds.), Nutrients in Dairy and their Implications on Health and Disease, 2017 (pp. 205-211): Academic Press.
Lam H-Y, Van Hoffen E, Michelsen A, Guikers K, Van Der Tas CHW., Bruijnzeel-Koomen CAFM, & Knulst AC. Cow's milk allergy in adults is rare but severe: both casein and whey proteins are involved. Clinical & Experimental Allergy, 2008, 38(6), 995-1002. doi:10.1111/j.1365-2222.2008.02968.x
Fritz KL, Seppanen CM, Kurzer MS, & Csallany AS. The in vivo antioxidant activity of soybean isoflavones in human subjects. Nutrition Research, 2003, 23(4), 479-487, doi:10.1016/S0271-5317(03)00005-8
Rahman Mazumder MA, & Hongsprabhas P. Genistein as antioxidant and antibrowning agents in in vivo and in vitro: A review. Biomedicine & Pharmacotherapy, 2016, 82, 379-392, doi: 10.1016/j.biopha.2016.05.023.
Serrano JC, Martín-Gari M, Cassanye A, Granado-Serrano AB, Portero-Otín M. Characterization of the post-prandial insulinemic response and low glycaemic index of a soy beverage. PLoS ONE, 2017, 12(8): e0182762, doi: 10.1371/journal.pone.0182762
