Physicochemical composition, microbiological quality and consumers’ acceptability of raw and pasteurized locally produced goat milk

Authors

  • Zarinah binti Zakaria Universiti Sultan Zainal Abidin
  • Wong Shi Yun Universiti Sultan Zainal Abidin
  • Nadiawati Alias Universiti Sultan Zainal Abidin
  • Siti Nuriah Mohd Noor Universiti Sultan Zainal Abidin
  • Abd Jamil Zakaria Universiti Sultan Zainal Abidin
  • Zakiah Mustapha Universiti Sultan Zainal Abidin
  • Napisah Hussin Universiti Sultan Zainal Abidin
  • Wan Rohani Wan Taib Universiti Sultan Zainal Abidin
  • Aryati Ahmad Universiti Sultan Zainal Abidin
  • Noor Aini Mohd Yusoff Universiti Sultan Zainal Abidin

DOI:

https://doi.org/10.11113/mjfas.v16n4.1842

Keywords:

Pasteurized goat milk, physicochemical composition, microbiological qualities, lactic acid bacteria, sensory evaluation

Abstract

The present study aimed to compare the raw and pasteurized goat milks in aspects of physicochemical composition, microbiological quality, and stability of lactic acid bacteria. Consumers’ acceptability to locally produced goat milk was also determined. Raw and pasteurized (LTLT) Saanen goat milks were analyzed while commercial (HTST and homogenized) goat milk was used as control. While pasteurization (LTLT) showed no significant effect on fat, protein, lactose, and ash content of raw goat milk (p>0.05). LTLT pasteurization kept the physicochemical properties of goat milk such as pH and viscosity similar to raw goat milk (p>0.05), there was a significant difference of titratable acidity (p<0.05). After LTLT and HTST pasteurizations, goat milk showed an increase in lightness with a reduction in yellowness, as well as significant reduction in microbial load. Survivability of lactic acid bacteria (anaerobic) was not affected by both pasteurization process (p>0.05). All of the studied goat milks contain lactic acid bacteria, primarily Enterococcus sp. via molecular identification by using 16S rRNA primer. Thus, LTLT pasteurization process at 63°C, 30 minutes might be applied to develop fermented dairy products such as probiotic goat drinks. According to sensory evaluation, consumer showed acceptability to locally produced goat milk with preference to commercial goat milk samples. In conclusion, lactation stage of goats and thermal processing conditions applied need to be considered since it might affect the quality of goat milk produced. To sum, the current information of locally produced goat milk would be useful for the development and manufacture of goat milk products especially in local dairy industry.

References

Al-Hilphy, A. R. S., Ali, H. I. 2013. Milk flash pasteurization by the microwave and study its chemical, microbiological and thermo physical characteristics. Journal of Food Process Technology 4, 250.

Anema, S. G., Li, Y. 2003. Association of denatured whey proteins with casein micelles in heated reconstituted skim milk and its effect on casein micelle size. Journal of Dairy Research 70, 73–83.

Anonymous. 2019. Agriculture Ministry says aim to make Malaysia self- sufficient in milk by 2025. Downloaded from https://www.malaymail.com/news/malaysia/2019/08/15/agriculture-ministry-says-aims-to-make-malaysia-self-sufficient-in-milk-by/1780929. Accessed on 8 April 2020.

AOAC. 2000. Official Methods of Analysis of the Association of Official Analytical Chemists International. 17th Edition. MD, USA: Association of Analytical Communities, Gaithersburg. Armstrong.

Araujo, T. F., Ferreira, C. L. L. F. 2013. The genus Enterococcus as probiotic: safety concerns. Brazilian Archives of Biology and Technology 56 (3), 457-466.

Armstrong, H. C. 1995. Breastfeeding as the foundation of care. Food and Nutrition Bulletin 16(4): 299-312.

Bakshi, A. K., Smith, D. E. 1984. Effect of fat content and temperature on viscosity in relation to pumping requirements of fluid milk products. Journal of Dairy Science 67, 1157- 1160.

Banik, S. K., Kamal, D., Uddin, Md. A. 2014. Microbiological quality analysis of raw, pasteurized, UHT milk samples collected from different locations in Bangladesh. Stamford Journal of Microbiology 4, 5-8.

Bhardwaj, A., Malik, R. K., Chauhan, P. 2008. Functional and safety aspects of enterococci in dairy foods. Indian. Journal of. Microbiology 48, 317–325.

Browning, E., Lewis, M., Macdougall, D. 2001. Predicting safety and quality parameters for UHT-processed milks. International Journal of Dairy Technology 54, 111–120.

Chugh, A., Dipendra, K., Walkling-Ribeiro, M., Corredig, M., Duizer, L., Griffiths, M. W. 2014. Change in color and volatile composition of skim milk processed with pulsed electric field and microfiltration treatments or heat pasteurization. Multidisciplinary Digital Publishing Institute 3(2), 250–268.

Connor, C. 1995. Rural Dairy Technology. Ethiopia: International Livestock Research Institute.

Denis, K., Ivan, P., Miroslav, P., Tamara, V. 2016. The composition of goat milk in different types of farmings. Biotechnology in Animal Husbandry 32 (4), 403-412.

Devendra, C. 2007. Goats: biology, production and development in Asia. Academy of Sciences, Malaysia.

Eglezos, S., Huang, B., Dykes, Gary A., Fegan, N., Bell, K., Stuttard, E. 2008. A survey of the microbiological quality of frozen unpasteurized goats’ milk in Queensland, Australia. The Australian Journal of Dairy Technology 63(3), 79-81.

FAO. 2018. Small ruminants. Food and Agriculture Organization of the United Nations, Rome, Italy.

Fandialan, M.M., Davide, C.L. 2001. Goatmilk: the relationship of its pH with total titratable acidity. Food and Agriculture Organization of the United Nations, Rome, Italy.

Food Regulations. 1985. Food ACT 1983 and Food Regulations 1985 (Act 281), 105- 266. Petaling Jaya, Malaysia:Golden Books Centre Sdn. Bhd.

Forsback, L., Lindmark-Mansson, H., Andren, A., Akerstedt, M., Andree, L., Svennersten- Sjaunja, K. 2010. Day-to-day variation in milk yield and milk composition at the udder- quarter level. Journal of Dairy Science 93(8), 3569–3577.

GEA. 2006. Titratable acidity. GEA Niro Research Laboratory, Germany.

Hanchi, H., Mottawea, W., Sebei, K., Hammami, R. 2018. The genus Enterococcus: between probiotic potential and safety concerns— anupdate. Frontier in Microbiology 9, 1791.

Hanchi, H., Hammami, R., Kourda, R., Hamida, J. B., Fliss, I. 2014. Bacteriocinogenic properties and in vitro probiotic potential of enterococci from Tunisian dairy products. Archives of Microbiology 196, 331–344.

Hassan, M., Talukder, M., Sultana, S. 2010. Evaluation of the production characteristics of the Jamunapari goat and its adaptability to farm conditions in Bangladesh. Bangladesh Veterinarian 27(1).

Hayam, M.A., Fatma, A.M.H., Mona, A.M.A., A.K.E. 2014. Physicochemical Characteristics of Goat’s Milk. Life Science Journal 11(1s), 307-317.

Hidayat, H. 2017. Analysis of 16S rRNA gene lactic acid bacteria (LAB) isolate from Markisa fruit (Passiflora sp.) as a producer of protease enzyme and probiotics.

Huppertz, T., Kelly, A.L. 2009. Properties and constituents of cow milk. In Tamime, A. Y. (Eds.). Milk Processing and Quality Management United Kingdom: Blackwell Publishing Ltd. 23-43.

Islam, M. Z., Monalisa, K. Hoque, M. M. 2012. Effects of pectin on the processing and preservation of Strawberry (Fragari annanassa) jam and jelly. International Journal of Natural Science 2(1), 8-14.

Ismail, Y. S., Yulvizar, C., Mazhitov, B. 2018. Characterization of lactic acid bacteria from local cow´ s milk kefir. Conference Series: Earth and Environmental Science 130.

Janda, J. M., Abbott, S. L. 2007. 16S rRNA gene sequencing for bacterial identification in the diagnostic laboratory: pluses, perils, and pitfalls. Journal of Clinical Microbiology 45(9), 2761–2764.

Kandeel, S. A., Megahed, A. A., Ebeid, M. H., Constable, P. D. 2018. Ability of milk pH to predict subclinical mastitis and intramammary infection in quarters from lactating dairy cattle. Journal of Dairy Science 102, 1417–1427.

Kanwal, R., Ahmed, T., Mirza, B. 2004. Comparative analysis of quality of milk collected from buffalo, cow, goat and sheep of Rawalpindi/Islamabad Region in Pakistan. Asian Journal of Plant Sciences 3(3), 300-305.

Khalid, K. 2011. An overview of lactic acid bacteria. International Journal of Biosciences 1(3), 1-13.

Kompan, D., Komprej, A. 2012. The Effect of Fatty Acids in Goat Milk on Health, InTech, Solevia.

Lai, C. Y., Fatimah, A. B., Mahyudin, N. A., Saari, N., Zaman, M. Z. 2016. Physico-chemical and microbiological qualities of locally produced raw goat milk. International Food Research Journal 05011(1), 1241–1245.

Laukova, A., Kandricakova, A., Bunkova, L., Pleva, P., Scerbova, J. 2017. Sensitivity to enterocins of biogenic amine-producing faecal Enterococci from ostriches and pheasants. Probiotics Antimicrobial Proteins 9, 483–491.

Leite, C. C., Guimaraes, A. G., Assis, P. N., Silva, M. D., Andrade, C. S. 2002. Bacteriological quality of whole milk type “c” commercialized in Salvador. Bahia Revista Brasileira de Saude e Producao Animal 3, 21-25.

Lejeune, J. T., Schultz, P. R. 2008. Unpasteurized milk: a continued public health threat. Clinical Infectious Diseases 48(1), 93-100.

Lye, Y. L., Afsah-Hejri, L., Chang, W. S., Loo, Y. Y., Puspanadan, S., Kuan, C. H., Son, R. 2013. Risk of Escherichia coli O157: H7 transmission linked to the consumption of raw milk. International Food Research Journal 20(2), 1001-1005.

Macdonald, L. E., Bret,T. J., Kelton, D., Majowicz, S. E., Snedeker, K., Sargeant, J. M. 2001. A systematic review and meta- analysis of the effects of pasteurization on milk vitamins, and evidence for raw milk consumption and other health-related outcomes. Journal of Food Protection 74(11), 1814-1832.

Malik, N. S., Lani, M. N., Ahmad, F. T. 2018. Stability of lactic acid bacteria and physico- chemical properties of pasteurized cow’s and goat’s milk. In Jahan, M. D. S., & Mohamed, (Eds.), International Conference on Agriculture, Animal Sciences & Food Technology (p.151-158). Terengganu, Malaysia: UniSZA Press.

Maria, B. T. O, Gabriela, N. V., Vanerli, B., Luís, A. N. 2007. Screening and enumeration of lactic acid bacteria in milk using three different culture media in petrifilm aerobic count plates and conventional pour plate methodology. The Journal of Dairy Research 74(4),387-91.

Michaela, S., Reinhard, W., Gerhard, K., Christine, M. E. 2009. Cultivation of anaerobic and facultatively anaerobic bacteria from spacecraft-associated clean rooms. Applied and Environmental Microbiology 11(75), 3484-3491.

Mirtha, L. G. P. 2005. Enterococci in milk products. Master of Veterinary Studies. Massey University, Palmerston North, New Zealand.

Mourad, G., Bettache, G., Samir, M. 2014. Composition and nutritional value of raw milk: review. Issues in Biological Sciences and Pharmaceutical Research 2(10), 115-122.

Murphy, S. C. 2007. The laboratory pasteurization count-thermoduric bacteria in raw milk. Ithaca: Cornell University Press.

Nero, L. A., Beloti, V., Barros, M. A. F. 2000. Rapid and automated methods for indicators microorganisms enumeration in milk - use in Brazil. Journal of Agricultural Sciences 21(1), 115-126.

Oliveira, L. P., Barros, L. S. S., Silva, V. C., Cirqueira, M. G. 2011. Study of Staphylococcus aureus in raw and pasteurized milk consumed in the Reconcavo area of the State of Bahia, Brazil. Journal of Food Process and Technology 2, 128.

Oliver, S. P., Boor, K. J., Murphy, S. C., Murinda, S. E. 2009. Food safety hazards associated with consumption of raw milk. Foodborne Pathogen Disease 6(7), 793-806.

Ogola, H., Shitandi, A., Nanua, J. 2007. Effect of mastitis on raw milk composition quality. Journal of Veterinary Science 8(3), 237-242.

Oscar F. H. S., Mauricio, V. P., Norma, M. D. L. F., Dennis, K. B., Jose, E. B. 2016. Bacteriocinogenic bacteria isolated from raw goat milk and goat cheese produced in the center of Mexico. Indian Journal of Microbiology 56(3), 301–308.

Park, Y. W. 2007. Hypoallergenic and therapeutic significance of goat milk. Small Ruminant Research 14, 151-159.

Patel, J. 2001. 16S rRNA gene sequencing for bacterial pathogen identification in the clinical laboratory. Molecular Diagnosis 6(4), 313–321.

Perez-Chabela, M. D. L., Alfonso, T., Isabel, G. 2008. Evaluation of thermotolerant capacity of lactic acid bacteria isolated from commercial sausages and the effects of their addition on the quality of cooked sausages. Ciencia e Tecnologia de Alimentos 28(1), 132-138.

Roman, P., Kumbar, V., Nedomova, S., Sustova, K. 2015. Effect of goat milk analytical properties on its viscosity and conductivity. Czech Republic: Mendel University.

Sachin, S.L., Aparnathi, K.D., Mehta, B., Suresh, V. 2017. Goat milk in human nutrition and health – a review. International Journal of Current Microbiolology Applied Science 6(5), 1781-1792.

Satomi, K., Chihiro, O., Hajime, T., Burenqiqige, Misato, K., Fumina, S., Ayaka, N., Shimaa, M.M., Takashi, K., Bon, K. 2018. Evaluation of Petrifilm Lactic Acid Bacteria Plates for Counting Lactic Acid Bacteria in Food. Journal of Food Protection 81(6),1030-1034.

Serio, A., Chaves-López, C., Paparella, A., Suzzi, G. 2010. Evaluation of metabolic activities of enterococci isolated from Pecorino Abruzzese cheese. International Dairy Journal 20, 459–464.

Solah V. A., Staines V., Honda S., Limley H. A. 2007. Measurement of milk colour and composition: effect of dietary intervention on western Australian Holstein-Friesian cow’s milk quality. Journal of Food Science 72, 560–566.

Stevens, J. (2011). 1kb DNA Ladder, Promega Corporation. Downloaded fromhttps://www.promega.media/wdn/coa/G5711-_0000189942.pdf?f =zqpzwamc9o. Accessed on 28 April 2019.

Tilahun, B., Tesfaye, A., Muleta, D., Bahiru, A., Terefework, Z., Wessel, G. 2018. Isolation and molecular identification of lactic acid bacteria using 16s rRNA genes from fermented teff (Eragrostis tef (Zucc.)) dough. International Journal of Food Science 1–7.

USDA. 2018. National nutrient database for standard reference. United States Department of Agriculture, Agricultural Research Service, USA.

Wanjekeche, E., Macosore, Z., Kiptanui, A., Lobeta, T. 2016. Quality and consumer acceptability of goat milk with respect to goat breed and lactation stage. African Crop Science Journal 24, 95–99.

Yang, S. C., Lin, C. H., Sung, C. T., Fang, J. Y. 2014. Antibacterial activities of bacteriocins: application in foods and pharmaceuticals. Frontier in Microbiology 5, 241.

Yangilar, Filiz. 2013. As a potentially functional food: goats’ milk and products. Journal of Food and Nutrition Research 1(4), 68–81.

Zenebe, T., Nejash, A., Tadele, K., Girma, K., Wollega. 2014. Review on medicinal and nutritional values of goat milk. Nature and Science 3(3), 30–39.

Downloads

Published

25-08-2020