Effects of magnesium ions in microbial cells adhesion of attached growth system for the enhancement of biogas production
DOI:
https://doi.org/10.11113/mjfas.v16n1.1643Keywords:
Biogas, biofilm, thermophilic, magnesium, immobilisationAbstract
This research aims to improve the biogas production by employing cell immobilisation technique under thermophilic conditions. The thermophilic fermentative biogas production was carried out by immobilising the anaerobic sludge obtained from palm oil mill treatment plant on granular activated carbon (GAC) in repeated batch mode. Different concentration of magnesium ions (Mg2+) (0.25, 0.5, 0.75, 1.0 and 1.5 g/l) on biogas production was investigated at 60°C with an initial sucrose concentration of 5 g/l as feedstock. The effect of Mg2+ supplementation at the initial stage of immobilisation process is important to increase the formation of biofilm in the attached growth system. This study had found that Mg2+ could enhance the biogas production capacity with optimum Mg2+ concentration of 0.75 g/l.
References
O-Thong, S., Prasertsan, P., Karakashev, D., Angelidaki, I. 2008. High-rate continuous hydrogen production by Thermoanaerobacterium thermosaccharolyticum PSU-2 immobilized on heat-pretreated methanogenic granules. International Journal of Hydrogen Energy, 33(22): 6498–6508.
Pasternak, K., Kocot, J., Horecka, A. 2010. Biochemistry of Magnesium. Journal of Elementology, 15(3): 601-616.
Lutpi, N. A., Jahim, J. M., Mumtaz, T., Abdul, P. M., Nor, M. T. M. 2015. Physicochemical characteristics of attached biofilm on granular activated carbon for thermophilic biohydrogen production, RSC Advances, 5: 19382–19392.
Lutpi, N. A., Jahim, J. A., Mumtaz, T., Harun, S., Abdul, P. M. 2016. Batch and continuous thermophilic hydrogen fermentation of sucrose using anaerobic sludge from palm oil mill effluent via immobilisation technique. Process Biochemistry, 51(2): 297-307.
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., Smith, F. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28(3): 350-356.
Feng, Y., Li, G. H., Mei, H. Y., Zhang, L. S. 2013. The Effect of magnesium ions on the hydrogen-producing bacteria in the water during the ecological restoration process. Advanced Materials Research, 610-613: 264-267.
Webb, M. 1949. The effect of magnesium on the growth of bacteria in simple. The Influence of Magnesium on Cell Division, 3: 418-424.
Moreira, J., Gomes, L., Araújo, J., Miranda, J. 2013. The effect of glucose concentration and shaking conditions on Escherichia coli biofilm formation in microtiter plates. Chemical Engineering Science, 94:192-199.
Zhang, G., Cao, J., Mao, Z., 2009. Influence of Mg2+ on the growth and activity of sulfate reducing bacteria. Hydrometallurgy, 95: 127-134.
Ng, T. S., Desa, M. N. H., Sandai, D., Chong, P. P., Than, L. T. L. 2015. Phylogenetic and transcripts profiling of glucose sensing related genes in Candida Glabrata. Jundishapur. Journal of Microbiology, 8(11): e25177.
Stewart P. S., Costerton J. W. 2001. Antibiotic resistance of bacteria in biofilms. Lancet, 358: 135-138.
Hall, S., Costerton, J., Stoodley, P. 2004. Bacterial biofilms: From the natural environment to infectious diseases. Nature Reviews Microbiology, 22: 95-108.
