Stability of lactic acid bacteria and physico-chemical properties of pasteurized cow’s and goat’s milk


  • Nurfarhana Syed Malik Universiti Malaysia Terengganu
  • Mohd Nizam Lani Universiti Malaysia Terengganu
  • Fauziah Tufail Ahmad Universiti Malaysia Terengganu



Milk, cow, goat, pasteurization, physico-chemical, lactic acid bacteria


This study was done to determine the effect of pasteurization on the stability of lactic acid bacteria and its enzyme, and also its relation with physico-chemical properties in raw and pasteurized cow’s and goat’s milk. Most of the physico-chemical properties (pH, protein, ash and fat) were highest in pasteurized goat’s milk. The total viable count for plate count of the bacterial concentration was higher in both pasteurized cow’s and goat’s milk which were 2.48 log CFU/ml. This was followed by raw cow’s milk (1.59 log CFU/ml) and raw goat’s milk (0.65 log CFU/ml). There was no yeast and mould detected in both raw and pasteurized cow’s and goat’s milk, respectively. Lactic acid bacteria (LAB) was found significantly higher in raw milk compared to pasteurized milk and higher macronutrients (proximate composition) could be considered as one of the factors for the survival of LAB. Interestingly, based on API ZYM assay kit result, there were nine different enzymes were detected in all samples which were leucine arylamidase, valine arylamidase, cystine arylamidase, trypsin, α-chymotrypsin, naphthol-AS-BI-phosphohydrolase, α-glucosidase, β-glucosidase and acid phosphatise. This result revealed that different types of lactic acid bacteria were detected in treated and non-treated milk samples produced by different animals.


Author Biographies

Nurfarhana Syed Malik, Universiti Malaysia Terengganu

School of Food Science and Technology

Mohd Nizam Lani, Universiti Malaysia Terengganu

School of Food Science and Technology

Fauziah Tufail Ahmad, Universiti Malaysia Terengganu

School of Food Science and Technology


Abdul Elrahman, S. M. A., Ahmed, A. M. E. M. S., El Zubeir, I. E. M., El Owni, O. A. O., Ahmed, M. K. A. 2013. Effect of storage temperature on the microbiological and physicochemical properties of pasteurized milk. Annals. Food Sci. Technol. 14, 1-7.

Abebe, T., & Markos, T. 2009. Milk quality control. International Center for Agricultural Research in the Dry Areas (ICARDA). Technical Bulletin No. 2.

Anderson, M., Hinds, P., Hurditt, S., Miller, P., McGrowder, D., Alexander-Lindo, R. 2011. The microbial content of unexpired pasteurized milk from selected supermarkets in a developing country. Asian Pac. J. Trop. Biomed. 1, 205-211.

Arora, R., Bhojak, N., & Joshi, R. 2013. Comparative aspects of goat and cow milk. Int. J. Eng. Sci. Invent. 2(1), 7-10.

AOAC. 2000. Official Methods of Analysis of AOAC International, 17th edition. The Association of Official Analytical Chemists. Gaithersburg, MD, USA.

Azhari Ali, A. 2011. Isolation and identification of lactic acid bacteria from raw cow milk in Khartoum State, Sudan. Int. J. Dairy Sci. 6(1), 66-71.

Azizkhani M., & Tooryan F. 2017. Chemical and microbial quality of Iranian commercial pasteurized milk samples at their expiration date. J Food Qual Hazards Control. 4(2), 53-57.

Balthazar, C. F., Pimentel, T. C., Ferrão, L. L., Almada, C. N., Santillo, A., Albenzio, M., Mollakhalili, N., Mortazavian, A. M., Nascimento, J. S., Silva, M. C., Freitas, M. Q., Sant’Ana, A. S., Granato, D., Cruz, A. G. 2017. Sheep milk: Physicochemical characteristics and relevance for functional food development. Compr Rev Food Sci Food Saf. 16(2), 247-262.

Blagojev, N., Skrinjar, M., Veskovic-Moracanin, S., & Soso, V. 2012. Control of mould growth and mycotoxin production by lactic acid bacteria metabolites. Rom Biotechnol Lett. 17(3). 7219-7226.

Bluma, A., & Ciprovica, I. 2016. Non starter lactic acid bacteria in raw milk thermally treated milk and swiss type cheese. Res. Rural Dev. 1, 98-101.

Brock, T. D., & Madigan, M. T. (1988) Biology of microorganisms (5th Edition), Prentice Hall, Englewood Cliffs, N.J.

Wiegel, J. 1990. Temperature soans for growth: Hypothesis and discussion. FEMS Microbiol. Rev. 75(2-3), 155-169

Caprita, A., Caprita, R. and Cretescu, I. 2014. The effects of storage conditions on some physicochemical properties of raw and pasteurized milk. J. Agroaliment. Processes Technol. 20(2), 198-202.

Carminati, D., Tidona, F., Fornasari, M. E., Rossetti, L., Meucci, A., & Giraffa, G. 2014. Biotyping of cultivable lactic acid bacteria isolated from donkey milk. Lett Appl Microbiol. 59, 299-305.

Cerbulis, J., Park, O. W., & Farrell, J. R. 1982. Composition and distribution of lipids of goats milk. J. Dairy Sci. 65, 2301-2307.

Chipilev, N., Daskalov, H., Stoyanchev, T. 2016. Study on the prevalence of lipolytic yeasts and moulds in raw cow milk and white brined cheese. Bulg. J. Vet. Med. 19(2), 117-126.

Claeys, W. L., Cardoena, S., Daubeb, G., Blockc, J., Dewettinckd, K., Dierick, K., Zutter, L., Huyghebaert, A., Imberechts, H., Thiange, P. 2013. Raw or heated cow milk consumption: Risks and benefits. Food Control. 31(1), 251-262.

Dalie, D. K. D., Deschamps, A. M., Forget, F. R. 2010. Lactic acid bacteria - Potential for control of mould growth and mycotoxins. Food Control. 21(4), 370-380.

de Vrese, M., Stegelmann, A., Richter, B., Fenselau, S., Laue, C., Schrezenmeir, J. 2001. Probiotics--compensation for lactase insufficiency. Am. J. Clin. Nutr. 73(2), 421- 429.

Delavenne, E., Mounier, J., Déniel, F., Barbier, G., Le Blay, G. 2012. Biodiversity of antifungal lactic acid bacteria isolated from raw milk samples from cow, ewe and goat over one-year period. Int. J. Food Microbiol. 155(3), 185–190.

Dumalisile, P., R. Witthuhn, T. Jbritz. 2005. Impact of different pasteurization temperatures on the survival of microbial contaminants isolated from pasteurized milk. Int. J. Dairy Technol. 58(2): 74-82.

FAO. 2013. Milk and dairy products in human nutrition. In: Muehlhoff, E., Bennette, A. McMahon, D. (Eds). Food and Agriculture Organization of the United Nations, Rome.

Garnier, L., Valence, F. and Mourier, J. 2017. Diversity and control of spoilage fungi in dairy products: An update. Microorganisms. 5(3), 42.

Getaneh G, Mebrat A, Wubie A, Kendie H. 2016. Review on goat milk composition and its nutritive value. J. Nutr. Health Sci. 3(4), 1-10.

Hassan, R. A., El Zubeir, I. E. M., Babiker, S. A. 2007. Effect of pasteurization of raw camel milk and storage temperature on the chemical composition of fermented camel milk. Int. J. Dairy Sci. 2(2), 166-171.

Humble, M. W., King, A., Phillips, I. 1977. API ZYM: A simple rapid system for the detection of bacterial enzymes. J. Clin. Pathol. 30(3), 275–277.

Hutkins, R.W., Nannen, N. L. 1993. pH homeostasis in lactic acid bacteria. J. Dairy Sci. 76(8), 2354-2365.

Imran, M., Khan, H., Hassan, S. S., Khan, R. 2008. Physicochemical characteristics of various milk samples available in Pakistan. J Zhejiang Univ Sci B., volume 9 (7), 546-551.

Jenness, P. E. 1978. The nutritive value of dairy products. Dairy Industries Int. 43, 7-16.

John, C. B. 1996. A summary of titratable acidity [Internet]. The Dairy Research and Information Center (DRINC). The University of California. Retrieved from DRINC website:

Khedid, K., Faid, M., Mokhtari, A., Soulaymani, A., Zinedine, A. 2009. Characterization of lactic acid bacteria isolated from the one humped camel milk produced in Morocco. Microbiol Res. 164(1), 81–91.

Korhonen, H., & Pihlanto, A. 2007. Bioactive peptides from food proteins. In: Hui, Y. H. (Ed) Handbook of Food Products Manufacturing. Publisher, Place Published, pp. 5-38.

Lai, C. Y., Fatimah, A. B., Mahyudin, N. A., Saari, N., Zaman, M. Z. 2016. Physico-chemical and microbiological qualities of locally produced raw goat milk. Int. Food Res. J. 23(2), 739–750.

Law, B. A., and Kolstad, J. 1983. Proteolytic systems in lactic acid bacteria, Antonie Van Leeuwenhoek. 49(3), 225-245.

Ledenbacch, L. H., Marshall, R. T. 2010. Microbiological spoilage of dairy products Compendium of the microbiological spoilage of foods and beverages. Springer. 41-67 In: Sperber W., & Doyle, M. (Eds) Compendium of the Microbiological Spoilage of Foods and Beverages. Food Microbiology and Food Safety. Springer, New York, NY, pp. 41-67.

Lewis, M. J., & Deeth, H. C. 2009. Heat treatment of milk. In: Tamime, A. Y. (Ed.) Blackwell Publishing Ltd. UK. p.168 Milk processing and quality management. Society of Dairy Technology book series. Wiley-Blackwell, Oxford, pp. 168-204.

Lipińska, L. Klewick, R., Klewicka, E. Kołodziejczyk, K., Sójka, M., Nowak, A. 2016. Antifungal activity of Lactobacillus sp. bacteria in the presence of xylitol and galactosyl-xylitol. BioMed Res. Int. Article ID 5897486, 8 pp.

MacDonald, L. E., Brett, J., Kelton, D. 2001. A systematic review and meta-analysis of the effects of pasteurization on milk vitamins, and evidence for raw milk consumption and other health-related outcomes. J. Food Prot. 74(11), 1814-1832.

Macleod, B. Y. R. A., Snell, E. E. 1947. Some mineral requirement of the lactic acid bacteria. J. Biol. Chem. 170, 351-365.

Malacarne, M., Martuzzi, F., Summer, A., Mariani, P. 2002. Protein and fat composition of mare’s milk: Some nutritional remarks with reference to human and cow’s milk. Int. Dairy J. 12(11), 869–877.

Meinardi, C. A., Zalazar, C. A., Hynes, E. R., Candiotti, M. C., 2003. Incremento del rendimeinto del queso cremoso argentino por tratamiento de la leche a temperaturas y tiempos superiores a los de pasteurización. Argentine Journal of Lactology, Revista Argentina de Lactología. 22, 45-54.

Burgess, K. 2013. Milk and dairy products in human nutrition. Int. J. Dairy Technol. 67, 303-304.

Murphy, S. C. 2007. The laboratory pasteurization counts [Notes]. Dairy Food Science Notes. Cornell University. Retrieved from https://foodsafety.

O’Donnell, R., Holland, J., Deeth, H., Alewood, P. 2004. Milk proteomics. Int. Dairy J. 14(12), 1013−1023.

Ogola, H., Shitandi, A., and Nanua, J., 2007. Effect of mastitis on raw milk composition quality. J. Vet. Sci. 8(3), 237-242.

Quigley, L., O'Sullivan, O., Stanton, C., Beresford, T. P., Ross, R. P., Fitzgerald, G. F., Cotter, P. D. 2013. The complex microbiota of raw milk, FEMS Microbiol. 37(5), 664–698.

Patel, A., Shah, N., Prajapati, J.B. 2013. Biosynthesis of vitamins and enzymes in fermented foods by lactic acid bacteria and related genera. Crop J. Sci. Technol. 5(2), 85-91.

Raccach, M. 1985. Manganese and lactic acid bacteria. J. Food Prot. 48(10), 895–898.

Samarzija, D., Zamberlin, S., Pogacic, T. 2012. Psychotropic bacteria and milk and dairy products quality, Mljekarstvo/Dairy. 62(2), 77-95. Retrieved from

Soliman G. Z. A. 2005. Comparison of chemical and mineral content of milk from human, cow, buffalo, camel and goat in Egypt. Egypt J Hosp Med. 21, 116-130.

Stoyanovski, S., Gacoviski, Z., Antonova-Nikolova, A., Kirilow, N., Ivanova, I., Tenev, T., Hadjinesheva, V. 2013. API ZYM enzymatic profile of lactic acid bacteria isolated from traditional Bulgarian meat products “Lukanka”. Bulg. J. Agric. Sci. 19(2), 86–89.

Tamine, A. Y., Robinson, R. K. 1999. Yoghurt: Science and Technology. Woodhead Publication, Cambridge, England.

Taufiq, T.T., Hadisaputro, W. 2013. Fermented goat milk and cow milk produced by different starters of lactic acid bacteria: Quality studies. J. Agric. Sci. Technol. 3, 904-911.

Tesfay, T., Kebede, A., and Seifu, E. 2015. Physico chemical properties of cow milk produced and marketed in Dire Dawa Town, Eastern Ethiopia. Food Sci. Qual. Manag. 42, 56-61.

Teshome, G. 2015. Review on lactic acid bacteria function in milk fermentation and preservation. Afr. J. Food Sci. 9(4), 170–175.

Thomas, A., & Prasad, V. 2014. Thermoduric bacteria in milk - A review. Int. J. Sci. Res. 3(6), 2438-2442.

Troller, J. A., & Stinson, J. V. 1981. Moisture requirements for growth and metabolite production by lactic acid bacteria. Appl. Environ. Microbiol. 42(4), 682–687.

USDA. 2012. Introduction to the Microbiology of Food Processing. Food Safety and Inspection Service, United States Department of Agriculture. Small Plant News Guidebook Series, Washington, D.C.

Wanjala, G. W., Mathooko, F. M, Kutima, P. M., and Mathara, J. M. 2017. Microbiological quality and safety of raw and pasteurized milk marketed in and around Nairobi region. Afr. J. Food Agric. Nutr. Dev. 17(1), 11518-

Wassie, M., & Wassie, T. 2016. Isolation and identification of lactic acic bacteria from raw cow milk. Int. J. Adv. Res. Biol. Sci. 3(8), 44-49.

Wedajo B. 2015. Lactic acid bacteria: Benefits, selection criteria and probiotic potential in fermented food. J. Prob. Health. 3, 129.

Widyastuti, Y., Febrisiantosa, R, A. 2014. The role of lactic acid bacteria in milk fermentation, Food Nutr. Sci. 5(4), 435-442.

Xu, M., Wang, Y., Dai, D., Zhang, Y., Li, Y., and Wang, J. 2015. Comparison of growth and nutritional status in infants receiving goat milk based formula and cow milk based formula: A randomized, double-blind study. Food Nutr. Res. 59, 28613.

Zamberlin, S., Antunac, N., Havranek, J., Samar Zija, D.2012. Mineral elements in milk and dairy products. Mljekarstvo. 62(2), 111-125.






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