Potential mutant of Lentinula edodes with high yield of (1-3), (1-6-), β-D-glucan


  • Ibrahim Mahmood Universiti Kebangsaan Malaysia
  • Azhar Mohamad Malaysian Nuclear Agency
  • Fauzi Daud Universiti Kebangsaan Malaysia
  • Babul Airianah Othman Universiti Kebangsaan Malaysia
  • Douglas Law Universiti Malaysia Kelantan
  • Cheah Yew Hoong ZACH Biotech Depot Sdn. Bhd
  • Mushrifah Idris Universiti Kebangsaan Malaysia
  • Azwan Mat Lazim Universiti Kebangsaan Malaysia
  • Seng Joe Lim Universiti Kebangsaan Malaysia
  • Nur Hidayah Jamar Universiti Kebangsaan Malaysia
  • Herryawan Ryadi Eziwar Dyari Universiti Kebangsaan Malaysia
  • Nik Marzuki Sidik Universiti Malaysia Kelantan
  • Shazrul Fazry Universiti Kebangsaan Malaysia




DM, MM, mutation, ISSR-PCR, Lentinula edodes


Lentinula edodes or better known as Shiitake mushroom contains β-1,3;1,6-glucan as part of the cell wall components and studies have shown that it has the ability to enhance the immune system and work as anticancer. The aim of this study is to create potential strain of L. edodes with the high content of β-1,3;1,6-glucan, which has the potential for biotechnological purposes. L. edodes spores were irradiated with gamma ray (Ɣ-rays) and incubated, which formed monokaryon mycelium (MM). Compatible mating of mutated monokaryon formed mutant line dikaryon mycelium (MLDM) that were selected for genotypic and phenotypic comparison with the wild-type line dikaryon mycelium (WLDM). The concentration of β-1,3;1,6-glucan were measured using a commercial β-Glucan Megazyme Assay Kit (Yeast & Mushroom). Three MLDM, (A37, A26, and C07) 38.8, 36.0 and 34.5 %w/w of 100 mg respectively, significantly produced higher amount of β-1,3;1,6-glucan in comparison with WLDM (20.2 %w/w of 100 mg).

Author Biographies

Ibrahim Mahmood, Universiti Kebangsaan Malaysia

Fakulti Sains dan Teknologi

Fauzi Daud, Universiti Kebangsaan Malaysia

Fakulti Sains dan Teknologi

Babul Airianah Othman, Universiti Kebangsaan Malaysia

Fakulti Sains dan Teknologi

Douglas Law, Universiti Malaysia Kelantan

Faculty of Agro Based Industry

Mushrifah Idris, Universiti Kebangsaan Malaysia

Fakulti Sains dan TeknologI

Azwan Mat Lazim, Universiti Kebangsaan Malaysia

Fakulti Sains dan Teknologi

Seng Joe Lim, Universiti Kebangsaan Malaysia

Fakulti Sains dan Teknologi

Nur Hidayah Jamar, Universiti Kebangsaan Malaysia

Fakulti Sains dan Teknologi

Herryawan Ryadi Eziwar Dyari, Universiti Kebangsaan Malaysia

Faculty of Agro Based Industry

Nik Marzuki Sidik, Universiti Malaysia Kelantan

Fakulti Sains dan Teknologi

Shazrul Fazry, Universiti Kebangsaan Malaysia

Fakulti Sains dan Teknologi


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