Immunohistochemical detection of Def6 protein in oral cancer


  • Nyi Mas Siti Purwaningsih Universiti Teknologi MARA
  • Khor Goot Heah Universiti Teknologi MARA
  • Nik Mohd Mazuan Nik Mohd Rosdy Universiti Teknologi MARA
  • Effat Omar Universiti Teknologi MARA



Def6, immunohistochemistry, oral cancer, protein expression


Nowadays oral cancer had become global health issue and reach 21st rank in Malaysian population. Def6 or IRF-4-binding proteins (IBP) that are mostly produced in lymph node and thymus are associated with cell survival and cell proliferation. Def6 protein expression is often found in autoimmune diseases, but current studies also found Def6 in some cancers. In this study, we would like to detect Def6 protein expression in oral cancer. 36 formalin-fixed paraffin embedded tissue were selected for both test group (oral cancer) and control group (normal oral mucosa). The cases were retrieved from the archives of faculty of dentistry, University of Malaya, Kuala Lumpur. Immunohistochemistry (IHC) staining was manually performed using Def6 antibody (Abcam 1:300) and assessed quantitatively (positivity and staining intensity). Positive and negative controls were used to validate the IHC run. All data were then analysed using SPSS version 25.0. Control group is negative for Def6 expression. 29 cases (82.86%) of oral cancer were Def6 positive with 2.86% weak staining, 5.71% moderate staining and 74.28% had strong staining intensity. There is significant different between Def6 expression in normal oral mucosa and oral cancer (p<0.05). Thus, we postulate that Def6 expression plays a big role in carcinogenesis of human oral cancer.

Author Biographies

Nyi Mas Siti Purwaningsih, Universiti Teknologi MARA

Department of Preclinical Science, Faculty of Dentistry

Khor Goot Heah, Universiti Teknologi MARA

Oral Cancer and Maxillofacial Research Group, Department of Preclinical Science, Faculty of Dentistry

Nik Mohd Mazuan Nik Mohd Rosdy, Universiti Teknologi MARA

Department of Oral and Maxillofacial Diagnostic and Medicine, Faculty of Dentistry

Effat Omar, Universiti Teknologi MARA

Department of Pathology, Faculty of Medicine


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