Effects of pressure variation and dynamic extraction time in supercritical fluid extraction (SFE) with cosolvent on bioactive compounds from Orthosiphon stamineus


  • Masniza Mohamed Mahmood Universiti Kebangsaan Malaysia
  • Wan Ramli Wan Daud Universiti Kebangsaan Malaysia
  • Masturah Markom Universiti Kebangsaan Malaysia
  • Che Nurul Ain Nadirah Che Mansor Universiti Kebangsaan Malaysia
  • Jalifah Latif Universiti Kebangsaan Malaysia




Orthosiphon stamineus, supercritical fluid extraction, cosolvent, herbal extraction


Supercritical Fluid Extraction (SFE) is an advance method used in extracting bioactive compounds from plants. However, carbon dioxide used as an extraction solvent in SFE has limitation in extracting polar compounds. By adding a small amount of co-solvent (polar solvent) to extract wide range (polar to non-polar) of bioactive compounds, thereby affecting the extract yields. Pressure and dynamic time play important roles in this technique. In this study, the effects of pressure variation and continuous dynamic extraction time on the bioactive compounds from Orthosiphon stamineus, which were rosmarinic acid, 3’-hydroxy-5,6,7,4’-tetramethoxyflavone, sinensetin, and eupatorin using ethanol (co-solvent) modified CO2 SFE were discussed. The pressure variations studied were included 150, 225, and 300 bar with continuous dynamic extraction. The co-solvent used was 10% (v/v) of absolute ethanol with a flow rate at 4 mL/min. The quantification of bioactive compounds was done using HPLC. The variation of pressure and dynamic time significantly affected the total component yields of targeted bioactive compounds obtained after the extraction process, as it was increased along with increasing of extraction pressure and time. The total yields of bioactive compounds at different pressures were 4.08% (150 bar), 4.18% (225 bar) and 4.51% (300 bar). The application of SFE with co-solvent and the relationship with the yields of targeted bioactive compounds obtained from of O.stamineus were given.

Author Biographies

Masniza Mohamed Mahmood, Universiti Kebangsaan Malaysia

Jabatan Kejuruteraan Kimia dan Proses, Fakulti Kejuruteraan dan Alam Bina

Wan Ramli Wan Daud, Universiti Kebangsaan Malaysia

Jabatan Kejuruteraan Kimia dan Proses, Fakulti Kejuruteraan dan Alam Bina

Masturah Markom, Universiti Kebangsaan Malaysia

Jabatan Kejuruteraan Kimia dan Proses, Fakulti Kejuruteraan dan Alam Bina

Che Nurul Ain Nadirah Che Mansor, Universiti Kebangsaan Malaysia

Pusat Pengajian Sains Kimia, Fakulti Sains dan Teknologi

Jalifah Latif, Universiti Kebangsaan Malaysia

Pusat Pengajian Sains Kimia, Fakulti Sains dan Teknologi


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