Accumulation of polyphenols and flavonoids in Atriplex canescens (Pursh) Nutt stressed by heavy metals (zinc, lead and cadmium)

Authors

  • Khedim Ikram University of Abdelhamid Ibn Badis
  • Reguieg Yssaad Houcine Abdelhakim University of Abdelhamid Ibn Badis
  • Bülent Topcuoglu Akdeniz University Vocational School of Technical Sciences
  • Osmane Badiaa University of Abdelhamid Ibn Badis
  • Tadjouri Houria University of Abdelhamid Ibn Badis

DOI:

https://doi.org/10.11113/mjfas.v16n3.1329

Keywords:

Heavy metals, Atriplex canescens (Pursh) Nutt, Total polyphenols, Flavonoids.

Abstract

Pollution of the environment and soils by heavy metals is one of the major problems of our time. Our study was to determine the effect of five doses for : zinc, lead and cadmium (0, 2500, 5000, 7500, and 10000 ppm) applied to the Atriplex canescens after 60 days for two weeks. The results showed an increase in the total polyphenols and flavonoids, depending on the increasing concentration of heavy metals at the leaf and root levels. The leaves have total polyphenols and flavonoids higher than those of the roots. The highest levels of total polyphenols and flavonoids in the leaves (3.47 mg EAG/ gdry weight of total polyphenols and 3.70 mg EQ/g dry weight of flavonoids) were obtained at a dose of 10000 ppm (cadmium, lead) respectively. On the other hand, the highest levels of total polyphenols and flavonoids in the roots were obtained at the metallic dose of 10000 ppm for cadmium (3.38 mg EAG/ gdry weight of total polyphenols and 0.61 mg EQ/g dry weight of flavonoids). These results also underlined the important role of secondary metabolites (total polyphenols and flavonoids) in the defense against oxidative stress caused by metallic stress.

Author Biographies

Khedim Ikram, University of Abdelhamid Ibn Badis

Laboratory of biodiversity and conservation of water and soils, Faculty of Nature and Life Sciences

Reguieg Yssaad Houcine Abdelhakim, University of Abdelhamid Ibn Badis

Laboratory of biodiversity and conservation of water and soils, Faculty of Nature and Life Sciences

Bülent Topcuoglu, Akdeniz University Vocational School of Technical Sciences

Department of plant and animal

Osmane Badiaa, University of Abdelhamid Ibn Badis

Laboratory of biodiversity and conservation of water and soils, Faculty of Nature and Life Sciences

Tadjouri Houria, University of Abdelhamid Ibn Badis

Laboratory of biodiversity and conservation of water and soils, Faculty of Nature and Life Sciences

References

Sutherzann, S. 2001. Natural and Enhanced Remediation Systems. CRC Press, p440.

Remon, E. 2006. Tolérance et Accumulation Des Métaux Lourds Par La Végétation Spontanée Des Friches Métallurgiques : Vers de nouvelles Méthodes De Bio-Dépollution. Thèse de doctorat de l’Université de Jean Monnet., pp 157.

Nijveldt, R. J., E. Nood, D. E. Hoorn, P.G. Boelens, K. Norren, P. Leeuwen. 2001. Flavonoids: A review of probable mechanisms of action and potential applications. The American Journal of Clinical Nutrition, 74(4) : 418-425.

Le Houérou, H. N. 1992. The role of salt bushes (Atriplex spp) in arid lands rehabilitation in the Mediterranean basin. A review. Agroforestry systems, 18 : 107-148.

Wang, L. W., A. M. Showalter. 2004. Cloning and salt-induced, ABA independent expression of choline mono-oxygenage in Atriplex prostrata. Physiologia Plantarum, 120 : 405–412.

Mulas, M., G. Mulas. 2004. The potential for strategic use of plants of the genera Atriplex and Opuntia in the fight against desertification. Short and Medium - Term Priority Environmental Action Program. University of Studies of Sassari Research Group on Desertification., pp 112.

Hoagland, D. R., D. I. Arnon. 1938. The water-culture method for growing plants without soil. Circular. California Agricultural Experiment Station, 347: 1-39.

Singleton, V. L., J. A. Rossi. 1965. Colorimetry of total phenolics with phosphomolybdic- phosphotungstic acid reagents. American Journal of Enology and Viticulture., 16 : 144-158.

Bahorun, T., B. Gressier, F. Trotin, C. Brunete, T. Dine, J. Vasseur, J. C. Gazin, M. Pinkas, M. Luycky, M. Gazin. 1996. Oxygen species scavenging activity of phenolic extracts from hawthorn fresh plant organs and pharmaceutical preparations. Arzneimittel-Forschung., 46 :1086-1089.

Sgherri, C., M.F. Quartacci and F. Navari-Izzo. 2007. Early production of activated oxygen species in root apoplast of wheat following copper excess. Journal of Plant Physiology., 164(9) :1152-1160.

Macheix, J. J., A. Fleuriet, C. Jay-Allemand. 2005. Les composés phénoliques des végétaux: Un exemple de métabolites secondaires d'importance économique. Ed. Presses polytechniques et universitaires romandes, Lausanne., pp 4-5.

Anderson, C. M., A. Hallberg, T. Hogberg. 1996. Advances in the développement of pharmaceutical antioxidant drug. Food Chemistry, 28 : 65-180.

Hu, Z. Z., M. Narayanaswamy, K. E. Ravikumar, K. Vijay-Shanker, C. H. Wu. 2005. Literature mining and database annotation of protein phosphorylation using a rule-based System., pp 2759-2765.

Boudiaf, K. 2006. Etude des effets anti-xanthine oxydoreductase et anti -radicalaires des extraits des graines de Nigellasativa. Mémoire de magister -Université de Sétif.

Bartosz, G. 2003. Génération of reactive oxygen species in biological Systems. Comments on Toxicology, 9: 5-21.

Abdel-Aal, E. S. M., J. C. Young, I. Rabalski. 2006. Anthocyanin composition in black, blue, pink, and red cereal grains. Journal of Agricultural and Food Chemistry, 544-696.

Jonnala, R. S. 2010. Phenolics in the bran of waxy wheat and triticale.

Chaib, G. H., A. Bouchelaleg, R. Talbi. 2015. Etude phytochimique de quelques variétés de blé tendre (Triticumaestivum) et d’orge (Hordeumvulgare) et leurs activités biologiques. European Scientific Journal., 11(30) :1857 - 7881.

War, A. R, M. G. Paulraj, M. Y. War, S. Ignacimuthu. 2011. Differential defensive response of groundnut to Helicoverpaarmigera (Hubner) (Lepidoptera: Noctuidae). Acta Physiologiae Plantarum, 34 : 343–352.

Korkmaz, A., M. Zuzunlu, A. R. Demirkiran. 2007. Treatment with acetyl salicylic acid protects muskmelon seedlings against drought stress. Franciszedgorski institute of plant physiologie. Polish academy of Science. Krakaow, tyrkey

Smirnoff, N. 2005. Antioxidants and reactive oxygen species in plants. Oxford Blackwell Publishing., pp 53–86.

Walton, N. J., D. E. Brown 1999. Chemivals from plants, Perspectives on secondary products,World Scientific., pp 56-66.

Morris, C. J., J. R. Earl, C. W. Trenam, D. R. Blake. 1995. Reactive oxygen species and iron a dangerous partnership in inflammation. The International Journal of Biochemistry & Cell Biology, 27: 109-122.

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Published

15-06-2020