Biohydrogen from Sugarcane Bagasse Pretreated with Combined Alkaline and Ionic Liquid [DMIM]DMP
Keywords:biohydrogen, sugarcane bagasse, ionic liquid, [DMIM]DMP pretreatment, Enterobacter aerogenes
Biohydrogen attracts many attentions since it has many advantages as source of energy. Biohydrogen from sugarcane bagasse offers many advantages from economic and environmental point of view. This work aimed to study the production of hydrogen from sugarcane bagasse through enzymatic hydrolysis and fermentation using Enterobacter aerogenes. Pretreatment with ionic liquid [DMIM]DMP was carried out prior to hydrolysis. It was found that process with ionic liquid was able to shift the cellulose structure from crystalline cellulose to more amorphous cellulose. Alkaline pretreatment followed by ionic liquid conducted for 20 min at 120oC gave the lowest crystallinity index. This condition also gave the highest total recovery of cellulose and hemicellulose, a condition that is very important for enzymatic hydrolysis to produce as much sugar as possible. Pretreatment condition was also found to give significant effect to the yield and type of monosaccharides produced from the hydrolysis process. Optimization of the pretreatment condition of the combined alkaline and ionic liquid [DMIM]DMP pretreatment was found to give significant effect to the ease of lignocellulosic substrate for its conversion to reducing sugar. The yield of hydrogen from the fermentation of the obtained sugar was 0.46 mole H2 per mole of glucose consumed.
Arantes, V.& Saddler, J. N. (2011). Cellulose accessibility limits the effectiveness of minimum cellulase loading on the efficient hydrolysis of pretreated lignocellulosic substrates. Biotechnology for Biofuels, 4, 3.
Argun, H., Kargi, F., Kapdan, I. K., Oztekin, R. (2008). Batch dark fermentation of powdered wheat starch to hydrogen gas: Effects of the initial substrate and biomass concentrations. International Journal of Hydrogen Energy, 33(21), 6009–6115.
Mäki-Arvela, P., Anugwom, I., Virtanen, P., Sjöholm, R., Mikkola, J. P.. (2010). Dissolution of lignocellulosic materials and its constituents using ionic liquids-A review. Industrial Crops and Products, 32(3), 175–201.
Azubuike, C. P., Rodríguez, H., Okhamafe, A. O., Rogers, R. D. (2011). Physicochemical properties of maize cob cellulose powders reconstituted from ionic liquid solution. Cellulose, 19(2), 425-433.
Bian, J., Peng, F., Peng, X. P., Xiao, X., Peng, P., Xu, F., Sun, R. C. (2013). Effect of [Emim]Ac pretreatment on the structure and enzymatic hydrolysis of sugarcane bagasse cellulose. Carbohydrate Polymer, 100, 211-217.
Chaumont, A. & Wipff, G. (2007). Solvation of big spherical solutes in room temperature ionic liquids and at their aqueous interface: A molecular dynamics simulation study. Journal of Molecular Liquids, 131–132, 36–47.
Datta, R. (1981). Acidogenic fermentation of lignocellulose-acid yield and conversion of components. Biotechnology and Bioengineering, 23, 2167-2170.
Fu, D., Mazza, G. (2011). Optimization of processing conditions for the pretreatment of wheat straw using aqueous ionic liquid. Bioresource Technology, 102(17), 8003-8010.
Kuo, C. H., Lee, C. K. (2009). Enhancement of enzymatic saccharification of cellulose by cellulose dissolution pretreatments. Carbohydrate Polymers, 77(1), 41–46.
Liebert, T. (2010). Cellulose solvents- remarkable history, bright future. In T. F. Liebert, T. J. Heinze, & K. J. Edgar (Eds.), Cellulose solvents:For analysis, shaping and chemical modification (pp. 3–54). New York: Oxford University Press.
Liu, W., Hou, Y., Wu, W., Ren, S., Wang, W. (2012). Complete conversion of cellulose to water soluble substance by pretreatment with ionic liquids. Korean Journal of Chemical Engineering, 29(10), 1403-1408.
Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31(3), 426-428.
Muharja, M, Junianti, F, Ranggina, D., Nurtono, T., Widjaja, A. (2018). An integrated green process: Subcritical water, enzymatic hydrolysis, and fermentation, for biohydrogen production from coconut husk. Bioresource Technology, 249, 268–275.
Park, S., Baker, J. O., Himmel, M. E., Parilla, P. A., Johnson, D. K. (2010). Cellulose crystallinity index: Measurement techniques and their impact on interpreting cellulase performance. Biotechnology for Biofuels, 3(10), 1-10.
Poletto, M., Ademir J. Z., Maria, M. C. F., Ruth M C. S. (2012). Thermal decomposition of wood components and cellulose crystallite size. Bioresource Technology, 109, 148-153.
George, J. M. R., Martin, C., da Silva, V. F. N., Gómez, E. O., Goncalves, A. R. (2011). Mass balance of pilot-scale pretreatment of sugarcane bagasse by steam explosion followed by alkaline delignification. Bioresource Technology, 111, 447-452.
Sangian, H. F., Kristian, J., Rahma, S., Agnesty, S. Y., Gunawan, S., Widjaja, A.. (2015a). Comparative study of the preparation of reducing sugars hydrolyzed from high-lignin lignocellulose pretreated with ionic liquid, alkaline solution and their combination. Journal of Engineering and Technological Sciences, 47(2), 137-148.
Sangian, H. F., Kristian, J., Rahma, S., Dewi, H. K., Puspasari, D. A., Agnesty, S. Y., Gunawan, S., Widjaja, A. (2015b). Preparation of reducing sugar hydrolyzed from high-lignin coconut coir dust pretreated by the recycled ionic liquid [mmim][dmp] and combination with alkaline. Bulletin of Chemical Reaction Engineering & Catalysis, 10(1), 8-22.
Saratale, G. D., Chen, S. D., Lo, Y. C., Saratale, R. G., Chang, J. S. (2008). Outlook of biohydrogen production from lignocellulosic feedstock using dark fermentation. A review. Journal of Scientific and Industrial Research, 67(11), 962-978.
Sen, S. M., Binder, J. B., Raines, R. T., Maravelias, C. T. (2012). Conversion of biomass to sugars via ionic liquid hydrolysis: process synthesis and economic evaluation. Biofuels, Bioproducts,& Biorefining, 6(4), 444–452.
Sghaier, A .E. O. B., Chaabouni, Y., Msahli, S., Sakli, F. (2012). Morphological and crystalline characterization of NaOH and NaOCl treated Agave americana L. fiber. Industrial Crops and Products, 36(1), 257–266.
Shanmugam, S., Hari, A., Ulaganathan, P., Yang, F., Krishnaswamy, S., Wu, Y.R. (2018). Potential of biohydrogen generation using the delignified lignocellulosic biomass by a newly identified thermostable laccase from Trichoderma asperellum strain BPLMBT1. Int. J. Hydrogen Energy, 43, 3618–3628.
Sun, Y., Zhuang, J., Lin, L., Ouyang, P. (2009). Clean conversion of cellulose into fermentable glucose. Biotechnology Advances, 27(5), 625-632.
Widjaja, A., Agnesty, S. Y., Sangian, H. F., Gunawan, S. (2015). Application of ionic liquid [DMIM]DMP pretreatment in the hydrolysis of sugarcane bagasse for biofuel production. Bulletin of Chemical Reaction Engineering & Catalysis, 10(1), 70-77.
Yang, F., Li, L., Li, Q., Tan, W., Liu, W. & Xian, M. (2010). Enhancement of enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media by ultrasonic intensification. Carbohydrate Polymers, 81(2), 311-316.
Zhao, D., Li, H., Zhang, J., Fua, L., Liu, M., Fua, J., Ren, P. (2012). Dissolution of cellulose in phosphate-based ionic liquids. Carbohydrate Polymer, 87(2), 1490-1494.
Zhi, S., Liu, Y., Yu, X., Wang, X., Lu, X. (2012). Enzymatic hydrolysis of cellulose after pretreated by ionic liquids: focus on one-spot process. Energy Procedia, 14, 1741-1747.
Zhu, Z., Zhu, M., Wu, Z. (2012). Pretreatment of sugarcane bagasse with NH4OH–H2O2 and ionic liquid for efficient hydrolysis and bioethanol production. Bioresource Technology, 119, 199–207.