Antibacterial activity of copper exchanged zeolite Y synthesized from rice husk ash
DOI:
https://doi.org/10.11113/mjfas.v14n0.1280Keywords:
Zeolite Y, copper ion, rice husk ash, hydrothermal, antibacterial agentAbstract
Increasing problems with antibacterial agent primarily bacterial resistance and environmental pollution due to the high release of antibacterial agents in water necessitates the development of new and effective antibacterial agents. One of the techniques that can be used to overcome these problems is by immobilizing antibacterial compounds or any related compounds on the carrier system such as by using zeolite. In this study, zeolite Y was synthesized from rice husk ash as silica source by using hydrothermal technique and it was used as a carrier system for antibacterial copper (Cu) ions. A series of Cu-exchanged zeolite Y was then prepared by loading with different concentrations of Cu ions (100 ppm, 600 ppm and 900 ppm of the Cu(NO3)2) on the synthesized zeolite Y. The Cu-exchanged zeolite Y was characterized by X-ray diffraction (XRD) and Fourier Transform Infrared spectroscopy (FTIR). These characterization techniques showed that the zeolite Y was synthesized in pure phase and had a good degree of crystallinity. Whereas, from the characterization results, zeolite Y was successfully loaded with different concentrations of Cu ions and no structural changes happen after modification. The antibacterial activity of the samples was determined through disc diffusion technique (DDT) against Gram positive bacteria (Staphylococcus aureus ATCC 6538 and Enterococcus faecalis ATCC 29212) and Gram negative bacteria (Escherichia coli ATCC 11229 and Pseudomonas aeruginosa ATCC 15442). Based on the antibacterial results, the synthesized zeolite Y loaded with 900 ppm of Cu2+ showed the highest antibacterial activity compared to that of loaded with 100 ppm and 600 ppm of Cu2+. The higher the Cu concentration on the zeolite Y resulted in the higher antibacterial activity against wide spectrum of bacteria. As a conclusion, synthesized zeolite Y from rice husk ash could be a carrier system for antibacterial Cu ions and it has the potential for the application as antibacterial agents.
References
Alswat, A. A., Ahmad, M. B., Saleh, T. A., Hussein, M. Z. B., Ibrahim, N. A. 2016. Effect of zinc oxide amounts on the properties and antibacterial activities of zeolite/zinc oxide nanocomposite. Mater. Sci. Eng. C 68, 505-511.
Alswat, A. A., Ahmad, M. B., Hussein, M. Z., Ibrahim, N. A., Saleh, T. A. 2017. Copper oxide nanoparticles-loaded zeolite and its characteristics and antibacterial activities. J. Mater. Sci. Technol. 33, 889-896.
Cai, X., Zhang, B., Liang, Y., Zhang, J., Yan, Y., Chen, X., Wu, Z., Liu, H., Wen, S., Tan, S., Wu, T. 2015. Study on the antibacterial mechanism of copper ion-and neodymium ion-modified α-zirconium phosphate with better antibacterial activity and lower cytotoxicity. Colloids Surf. B. 132, 281-289.
Charkhi, A., Kazemeini, M., Ahmadi, S. J., Kazemian, H. 2012. Fabrication of granulated NaY zeolite nanoparticles using a new method and study the adsorption properties. Powder Technol. 231, 1-6.
Erdem, E., Karapinar, N., Donat, R. 2004. The removal of heavy metal cations by natural zeolites. J. Colloid Interface Sci. 280, 309-314.
Ferreira, L., Guedes, J. F., Almeida-Aguiar, C., Fonseca, A. M., Neves, I. C. 2016. Microbial growth inhibition caused by Zn/Ag-Y zeolite materials with different amounts of silver. Colloid Surface B.142, 141-147.
Ghasemi, Z., Younesi, H., Kazemian, H. 2011. Synthesis of nanozeolite sodalite from rice husk ash without organic additives. T. Can. J. Chem. Eng. 89, 601-608.
Hanim, S. A. M., Malek, N. A. N. N., Ibrahim, Z. 2017. Analyses of surface area, porosity, silver release and antibacterial activity of amine-functionalized, silver-exchanged zeolite NaY. Vacuum, 143, 344-347.
Malachová, K., Praus, P., Rybková, Z., Kozák, O. 2011. Antibacterial and antifungal activities of silver, copper and zinc montmorillonites. Appl. Clay Sci. 53, 642-645.
Malek, N. A. N. N., Williams, C. D., Dhanabal, S., Bhall, H. S., Ibrahim, N. 2014. Natural clinoptilolite and chabazite as carrier for antibacterial agents of cetylpyridinium chloride (CPC) and silver. Appl. Mech. Mater. 606, 29-33.
Milenkovic, J., Hrenovic, J., Matijasevic, D., Niksic, M., Rajic, N. 2017. Bactericidal activity of Cu-, Zn-, and Ag-containing zeolites toward Escherichia coli isolates. Environ. Sci. Pollut. Res. 24, 20273-20281.
Mölstad, S., Löfmark, S., Carlin, K., Erntell, M., Aspevall, O., Blad, L., Hanberger, H., Hedin, K., Hellman, J., Norman, C., Skoog, G., Stalsby-Lundborg, C., Wisell, K.T., Ahren, C., Cars, O. 2017. Lessons learnt during 20 years of the Swedish strategic programme against antibiotic resistance. B. World Health Organ. 95, 764.
Pode, R. 2016. Potential applications of rice husk ash waste from rice husk biomass power plant. Renew. Sust. Energ. Rev. 53, 1468-1485.
Saad, N. S. S. M., Malek, N. A. N. N., Chong, C. S. 2016. Antimicrobial activity of copper kaolinite and surfactant modified copper kaolinite against gram positive and gram negative bacteria. Jurnal Teknologi, 78(3-2), 127-132.
Saengmee-Anupharb, S., Srikhirin, T., Thaweboon, B., Thaweboon, S., Amornsakchai, T., Dechkunakorn, S., Suddhasthira, T. 2013. Antimicrobial effects of silver zeolite, silver zirconium phosphate silicate and silver zirconium phosphate against oral microorganisms. Asian Pac. J. Trop. Med. 3, 47-52.
Salim, M. M., Malek, N. A. N. N. 2016. Characterization and antibacterial activity of silver exchanged regenerated NaY zeolite from surfactant-modified NaY zeolite. Mater. Sci. Eng. C, 59, 70-77.
Salim, M.M. Malek, N.A.N.N. 2017. Review of modified zeolites by surfactant and silver as antibacterial agents. J. Adv. Res. Mater. Sci. 36, 1-20.
Shen, X., Qiu, G., Yue, C., Guo, M., Zhang, M. 2017. Multiple copper adsorption and regeneration by zeolite 4A synthesized from bauxite tailings. Environ. Sci. Pollut. Res. 24, 21829-21835.
Treacy, M. M., Higgins, J. B. 2007. Collection of simulated XRD powder patterns for zeolites fifth (5th) revised edition. Amsterdam, the Netherlands: Elsevier.
Yao, Z. T., Ji, X. S., Sarker, P. K., Tang, J. H., Ge, L. Q., Xia, M. S., Xi, Y. Q. (2015). A comprehensive review on the applications of coal fly ash. Earth-Sci. Rev. 141, 105-121.
Yoon, K. Y., Byeon, J. H., Park, J. H., Hwang, J. 2007. Susceptibility constants of Escherichia coli and Bacillus subtilis to silver and copper nanoparticles. Sci. Total Environ. 373, 572-575.
Yusof, A. M., Nizam, N. A., Rashid, N. A. A. 2010. Hydrothermal conversion of rice husk ash to faujasite-types and NaA-type of zeolites. J. Porous Mater. 17, 39-47.
