Effectiveness of visible and ultraviolet light emitting diodes for inactivation of Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli: A comparative study


  • Sameen Ahmed Malik Universiti Teknologi Malaysia
  • Tan Tian Swee Universiti Teknologi Malaysia
  • Nik Ahmad Nizam Nik Malek
  • Azli Yahya
  • Takahiro Emoto
  • Masatake Akutagawa
  • Leong Kah Meng
  • Tan Jia Hou
  • Tengku Ahmad Iskandar Tengku Alang
  • Kelvin Ling Chia Hiik




Visible LED, UVA-LED, pathogens, inactivation effectiveness


The rapid use of ultraviolet light emitting diodes (UV-LEDs) in various disinfection applications is growing tremendously due to their advantages unachievable using UV lamps. In this study, a comparison of standard LED at 460 nm wavelength and UVA LED at 385 nm was conducted to determine their effectiveness in disinfection of frequently isolated pathogens in hospitals (Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli). Determination of disinfection efficiency was carried out by measuring inhibition zone. Effects of varied exposure time on the inactivation of pathogenic microorganisms was studied. The results demonstrated that LED does not have germicidal activities. The highest inactivation for UVA LED was achieved for Pseudomonas aeruginosa. Linear relationship was found between exposure time and log reduction. This study showed that UVA LEDs can effectively inactivate significantly higher number of microorganisms hence can be used in disinfection of various applications.

Author Biography

Tan Tian Swee, Universiti Teknologi Malaysia

PM Ir Dr 

Department of Biotechnology and Medical Engineering, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, Skudai 81310, Johor, Malaysia. 


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