Anticancer activity of novel soursop leaves active compound (sf-1603) through apoptotic induction in liver cancer
DOI:
https://doi.org/10.11113/mjfas.v14n2.1064Keywords:
Apoptosis, liver cancer, soursop (Annona muricata) leaves, targetted therapyAbstract
There are no effective standard treatments for unresectable stage of liver cancer have been established. These insights lead the pursuit of targeted therapeutic strategies for reactivating apoptosis to eradicate cancer cells. It was hypothesized that active compound in the soursop leaves will be able to induce apoptosis. Objective of the study is to explore the role of novel soursop leaves active compound (SF-1603) in apoptosis induction to find a new agent for liver cancer therapy. This study was use HepG2 cell line culture seeded in DMEM / F12. There were control group and 3 intervention group given SF-1603 with different concentration. Apoptosis detection was done using Terminal deoxynucleotidyl Transferase-mediated dUTP Nick End Labeling (TUNEL) method. Statistical analyses were conducted using linear regression. The result showed the cells undergoing apoptosis in the control group less than treatment groups. There was a strong positive correlation (r=0.847) between the rate of apoptosis and a concentration of SF-1603, reflected the apoptotic effect with a dose-dependent manner. The study conclude, the novel soursop leaves active compound SF-1603 had the strong ability to induce apoptosis on liver cancer cell line culture, so it can be used as a candidate for new agent for liver cancer therapy.References
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